B4DG68 (B4DG68_HUMAN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Recommended name: Nitric oxide synthase PIRNR PIRNR000333 EC=1.14.13.39 PIRNR PIRNR000333
Organism	Homo sapiens (Human) EMBL BAG57679.1
Taxonomic identifier	9606 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1206 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Produces nitric oxide (NO) By similarity. PIRNR PIRNR000333
Catalytic activity	2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. PIRNR PIRNR000333
Cofactor	Binds 1 FAD By similarity. PIRNR PIRNR000333 Binds 1 FMN By similarity. PIRNR PIRNR000333 Heme group By similarity. PIRNR PIRNR000333
Sequence similarities	Belongs to the NOS family. PIRNR PIRNR000333 Contains 1 PDZ (DHR) domain. RuleBase RU003354

Ontologies

Keywords
Ligand	Calmodulin-binding PIRNR PIRNR000333 FAD PIRNR PIRNR000333 FMN PIRNR PIRNR000333 Heme PIRNR PIRNR000333 Iron Metal-binding NADP PIRNR PIRNR000333
Molecular function	Oxidoreductase PIRNR PIRNR000333
Gene Ontology (GO)
Biological_process	nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	FMN binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro nitric-oxide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B4DG68 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: E4DBC7937045D909	FASTA	1,206	134,816
	10 20 30 40 50 60 MEDHMFGVQQ IQPNVISVRL FKRKVGGLGF LVKERVSKPP VIISDLIRGG AAEQSGLIQA 70 80 90 100 110 120 GDIILAVNGR PLVDLSYDSA LEVLRGIASE THVVLILRGP EGFTTHLETT FTGDGTPKTI 130 140 150 160 170 180 RVTQPLGPPT KAVDLSHQPP AGKEQPLAVD GASGPGNGPQ HAYDDGQEAG SLPHANGLAP 190 200 210 220 230 240 RPPGQDPAKK ATRVSLQGRG ENNELLKEIE PVLSLLTSGS RGVKGGAPAK AEMKDMGIQV 250 260 270 280 290 300 DRDLDGKSHK PLPLGVENDR VFNDLWGKGN VPVVLNNPYS EKEQPPTSGK QSPTKNGSPS 310 320 330 340 350 360 KCPRFLKVKN WETEVVLTDT LHLKSTLETG CTEYICMGSI MHPSQHARRP EDVRTKGQLF 370 380 390 400 410 420 PLAKEFIDQY YSSIKRFGSK AHMERLEEVN KEIDTTSTYQ LKDTELIYGA KHAWRNASRC 430 440 450 460 470 480 VGRIQWSKLQ VFDARDCTTA HGMFNYICNH VKYATNKGNL RSAITIFPQR TDGKHDFRVW 490 500 510 520 530 540 NSQLIRYAGY KQPDGSTLGD PANVQFTEIC IQQGWKPPRG RFDVLPLLLQ ANVNDPELFQ 550 560 570 580 590 600 IPPELVLEVP IRHPKFEWFK DLGLKWYGLP AVSNMLLEIG GLEFSACPFS GWYMGTEIGV 610 620 630 640 650 660 RDYCDNSRYN ILEEVAKKLN LDMRKTSSLW KDQALVEINI AVLYSFQSDK VTIVDHHSAT 670 680 690 700 710 720 ESFIKHMENE YRCRGGCPAD WVWIVPPMSG SITPVFHQEM LNYRLTPSFE YQPDPWNTHV 730 740 750 760 770 780 WKGTNGTPTK RRAIGFKKLA EAVKFSAKLM GQAMAKRVKA TILYATETGK SQAYAKTLCE 790 800 810 820 830 840 IFKHAFDAKV MSMEEYDIVH LEHETLVLVV TSTFGNGDPP ENGEKFGCAL MEMRHPNSVQ 850 860 870 880 890 900 EERKSYKVRF NSVSSYSDSQ KSSGDGPDLR DNFESAGPLA NVRFSVFGLG SRAYPHFCAF 910 920 930 940 950 960 GHAVDTLLEE LGGERILKMR EGDELCGQEE AFRTWAKKVF KAACDVFCVG DDVNIEKANN 970 980 990 1000 1010 1020 SLISNDRSWK RNKFRLTFVA EAPELTQGLS NVHKKRVSAA RLLSRQNLQS PKSSRSTIFV 1030 1040 1050 1060 1070 1080 RLHTNGSQEL QYQPGDHLGV FPGNHEDLVN ALIERLEDAP PVNQMVKVEL LEERNTALGV 1090 1100 1110 1120 1130 1140 ISNWTDELRL PPCTIFQAFK YYLDITTPPT PLQLQQFASL ATSEKEKQRL LVLSKGLQEY 1150 1160 1170 1180 1190 1200 EEWKWGKNPT IVEVLEEFPS IQMPATLLLT QLSLLQPRYY SISSSPHTHT LSETRPSPLP LSSQCC

References

[1]

"NEDO human cDNA sequencing project focused on splicing variants." Wakamatsu A., Yamamoto J., Kimura K., Ishii S., Watanabe K., Sugiyama A., Murakawa K., Kaida T., Tsuchiya K., Fukuzumi Y., Kumagai A., Oishi Y., Yamamoto S., Ono Y., Komori Y., Yamazaki M., Kisu Y., Nishikawa T. , Sugano S., Nomura N., Isogai T. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Tissue: Amygdala EMBL BAG57679.1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK294435 mRNA. Translation: BAG57679.1.
IPI	IPI00217225.
RefSeq	NP_000611.1. NM_000620.4. NP_001191142.1. NM_001204213.1. NP_001191143.1. NM_001204214.1. NP_001191147.1. NM_001204218.1.
UniGene	Hs.654410. Hs.684465. Hs.684466. Hs.684467. Hs.735734.
3D structure databases
ProteinModelPortal	B4DG68.
ModBase	Search...
Protocols and materials databases
DNASU	4842.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4842.
KEGG	hsa:4842.
Organism-specific databases
CTD	4842.
Phylogenomic databases
HOGENOM	HOG000220884.
HOVERGEN	HBG000159.
KO	K13240.
Family and domain databases
Gene3D	1.20.990.10. 1 hit. 3.90.340.10. 1 hit.
InterPro	IPR003097. FAD-binding_1. IPR001094. Flavdoxin. IPR008254. Flavodoxin/NO_synth. IPR023173. NADPH_Cyt_P450_Rdtase_dom3. IPR004030. NO_synthase_oxygenase_dom. IPR026009. NOS. IPR012144. NOS_met. IPR001478. PDZ. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
PANTHER	PTHR19384:SF5. PTHR19384:SF5. 1 hit.
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF02898. NO_synthase. 1 hit. PF00595. PDZ. 1 hit. [Graphical view]
PIRSF	PIRSF000333. NOS. 1 hit.
PRINTS	PR00369. FLAVODOXIN.
SMART	SM00228. PDZ. 1 hit. [Graphical view]
SUPFAM	SSF56512. NO_synthase_oxygenase_reg. 1 hit. SSF50156. PDZ. 1 hit. SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS50902. FLAVODOXIN_LIKE. 1 hit. PS60001. NOS. 1 hit. PS50106. PDZ. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	4842.
NextBio	18658.

Entry information

Entry name

B4DG68_HUMAN

Accession

Primary (citable) accession number: B4DG68

Entry history

Integrated into UniProtKB/TrEMBL:	September 23, 2008
Last sequence update:	September 23, 2008
Last modified:	April 3, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.