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B3WER5 (IF2_LACCB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Translation initiation factor IF-2
Gene names
Name:infB
Ordered Locus Names:LCABL_17860
OrganismLactobacillus casei (strain BL23) [Complete proteome] [HAMAP]
Taxonomic identifier543734 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length943 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00100.

Sequence similarities

Belongs to the IF-2 family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionInitiation factor
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 943943Translation initiation factor IF-2 HAMAP-Rule MF_00100
PRO_1000093796

Regions

Nucleotide binding453 – 4608GTP By similarity
Nucleotide binding499 – 5035GTP By similarity
Nucleotide binding553 – 5564GTP By similarity
Region447 – 595149G-domain HAMAP-Rule MF_00100

Sequences

Sequence LengthMass (Da)Tools
B3WER5 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: 01B5958EAC6C5C54

FASTA943103,151
        10         20         30         40         50         60 
MGKKRVYEFA KEMHVDNKDV IDIAKNLGIE VKNHMSSIDQ DQEAKIKGML SKQSAGKAPS 

        70         80         90        100        110        120 
SQAAKTPAKA AKTSSAAHKE AAKKPVAASA KSNDHADVAE HSQKNAKPAA KQENKPARSN 

       130        140        150        160        170        180 
KTSDGKIILS KSTILRPRST QTAHTNTNHN RGGNTASANN TANGRNSNRS NNNNNNRSAN 

       190        200        210        220        230        240 
NANRSGNNNR SNERNRNDRN RRFDNQRVTG PMPRAGRPTA ANGNPRPVAG NGGKFVKPAS 

       250        260        270        280        290        300 
ERQQPKRQEA VKPANAASKR SEQPRTERPR TEQPAATTNQ RFTKPAPVPA AAAPKPASAG 

       310        320        330        340        350        360 
SQDNRNSRRG GGNSNFGRSN SYGNRNGFNR NNRRNKKNKR RQQSAPKKEM PQRKERPLPE 

       370        380        390        400        410        420 
TLIYEVGMNA QDLGKILHRE PAELIKKLFM LGVMVNQNQS LDKDTIELLA TDYGIDAQEK 

       430        440        450        460        470        480 
VHEDISDLDK VFEEENKNQD NLQPRPPVVT IMGHVDHGKT TLLDKLRHTH VTEGEAGGIT 

       490        500        510        520        530        540 
QHIGAYQVKL RDRLITFLDT PGHAAFTNMR ARGADITDIV VLVVAADDGV MPQTIEAIHH 

       550        560        570        580        590        600 
AQAAKAPIIV AVNKIDKPGA NPDHVMEQLT EYGLIPEDWG GDTIFVKISA KFGKNIDELL 

       610        620        630        640        650        660 
EMILLEADVL ELKANPDQKA VGTVIEARLD KGKGPVATVL VQQGTLHTGD PIVVGNTFGR 

       670        680        690        700        710        720 
VRAMTNDHGR RVKDALPSMP VEITGINDVP QSADKFVVFA DERTARAAGE ERAKRAQEEE 

       730        740        750        760        770        780 
RKNTNHVTLD NLFETMKEGQ LKEVDVIIKA DVQGSVEALA GSLEKIEVKG VRVNIIHQAV 

       790        800        810        820        830        840 
GAINESDVTL AAASNAIIIG FNVRPTALAK AQAEQDDVDI RLHSVIYKAI EEVEAAMKGM 

       850        860        870        880        890        900 
LEPTYEEKVI GTVTVRETIP VSKVGTVVGG YVDSGYITRD AGVRLVRDGI VKYEGKLGSL 

       910        920        930        940 
RRFKDDVKEV RQGFELGLTI ENYNDIKVDD QIEAFTMEQV PVK 

« Hide

References

[1]"Lactobacillus casei BL23 complete genome sequence."
Maze A., Boel G., Bourand A., Loux V., Gibrat J.F., Zuniga M., Hartke A., Deutscher J.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BL23.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FM177140 Genomic DNA. Translation: CAQ66866.1.
RefSeqYP_001987724.1. NC_010999.1.

3D structure databases

ProteinModelPortalB3WER5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING543734.LCABL_17860.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAQ66866; CAQ66866; LCABL_17860.
GeneID6406655.
KEGGlcb:LCABL_17860.
PATRIC22213015. VBILacCas89204_1693.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0532.
HOGENOMHOG000076906.
KOK02519.
OMARSANNAN.
OrthoDBEOG67HJSV.

Enzyme and pathway databases

BioCycLCAS543734:GCHL-1763-MONOMER.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_B. IF_2_B.
InterProIPR000795. EF_GTP-bd_dom.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
ProtoNetSearch...

Entry information

Entry nameIF2_LACCB
AccessionPrimary (citable) accession number: B3WER5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 2, 2008
Last modified: May 14, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries