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Protein

Translation initiation factor IF-2

Gene

infB

Organism
Lactobacillus casei (strain BL23)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi453 – 4608GTPUniRule annotation
Nucleotide bindingi499 – 5035GTPUniRule annotation
Nucleotide bindingi553 – 5564GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: UniProtKB-HAMAP
  2. GTP binding Source: UniProtKB-KW
  3. translation initiation factor activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLCAS543734:GCHL-1763-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor IF-2UniRule annotation
Gene namesi
Name:infBUniRule annotation
Ordered Locus Names:LCABL_17860
OrganismiLactobacillus casei (strain BL23)
Taxonomic identifieri543734 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000001206 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 943943Translation initiation factor IF-2PRO_1000093796Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi543734.LCABL_17860.

Structurei

3D structure databases

ProteinModelPortaliB3WER5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini444 – 613170tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni453 – 4608G1By similarity
Regioni478 – 4825G2By similarity
Regioni499 – 5024G3By similarity
Regioni553 – 5564G4By similarity
Regioni589 – 5913G5By similarity

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0532.
HOGENOMiHOG000076906.
KOiK02519.
OMAiKSNDHAD.
OrthoDBiEOG67HJSV.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_B. IF_2_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERiPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3WER5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKRVYEFA KEMHVDNKDV IDIAKNLGIE VKNHMSSIDQ DQEAKIKGML
60 70 80 90 100
SKQSAGKAPS SQAAKTPAKA AKTSSAAHKE AAKKPVAASA KSNDHADVAE
110 120 130 140 150
HSQKNAKPAA KQENKPARSN KTSDGKIILS KSTILRPRST QTAHTNTNHN
160 170 180 190 200
RGGNTASANN TANGRNSNRS NNNNNNRSAN NANRSGNNNR SNERNRNDRN
210 220 230 240 250
RRFDNQRVTG PMPRAGRPTA ANGNPRPVAG NGGKFVKPAS ERQQPKRQEA
260 270 280 290 300
VKPANAASKR SEQPRTERPR TEQPAATTNQ RFTKPAPVPA AAAPKPASAG
310 320 330 340 350
SQDNRNSRRG GGNSNFGRSN SYGNRNGFNR NNRRNKKNKR RQQSAPKKEM
360 370 380 390 400
PQRKERPLPE TLIYEVGMNA QDLGKILHRE PAELIKKLFM LGVMVNQNQS
410 420 430 440 450
LDKDTIELLA TDYGIDAQEK VHEDISDLDK VFEEENKNQD NLQPRPPVVT
460 470 480 490 500
IMGHVDHGKT TLLDKLRHTH VTEGEAGGIT QHIGAYQVKL RDRLITFLDT
510 520 530 540 550
PGHAAFTNMR ARGADITDIV VLVVAADDGV MPQTIEAIHH AQAAKAPIIV
560 570 580 590 600
AVNKIDKPGA NPDHVMEQLT EYGLIPEDWG GDTIFVKISA KFGKNIDELL
610 620 630 640 650
EMILLEADVL ELKANPDQKA VGTVIEARLD KGKGPVATVL VQQGTLHTGD
660 670 680 690 700
PIVVGNTFGR VRAMTNDHGR RVKDALPSMP VEITGINDVP QSADKFVVFA
710 720 730 740 750
DERTARAAGE ERAKRAQEEE RKNTNHVTLD NLFETMKEGQ LKEVDVIIKA
760 770 780 790 800
DVQGSVEALA GSLEKIEVKG VRVNIIHQAV GAINESDVTL AAASNAIIIG
810 820 830 840 850
FNVRPTALAK AQAEQDDVDI RLHSVIYKAI EEVEAAMKGM LEPTYEEKVI
860 870 880 890 900
GTVTVRETIP VSKVGTVVGG YVDSGYITRD AGVRLVRDGI VKYEGKLGSL
910 920 930 940
RRFKDDVKEV RQGFELGLTI ENYNDIKVDD QIEAFTMEQV PVK
Length:943
Mass (Da):103,151
Last modified:September 2, 2008 - v1
Checksum:i01B5958EAC6C5C54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM177140 Genomic DNA. Translation: CAQ66866.1.
RefSeqiYP_001987724.1. NC_010999.1.

Genome annotation databases

EnsemblBacteriaiCAQ66866; CAQ66866; LCABL_17860.
KEGGilcb:LCABL_17860.
PATRICi22213015. VBILacCas89204_1693.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM177140 Genomic DNA. Translation: CAQ66866.1.
RefSeqiYP_001987724.1. NC_010999.1.

3D structure databases

ProteinModelPortaliB3WER5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi543734.LCABL_17860.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAQ66866; CAQ66866; LCABL_17860.
KEGGilcb:LCABL_17860.
PATRICi22213015. VBILacCas89204_1693.

Phylogenomic databases

eggNOGiCOG0532.
HOGENOMiHOG000076906.
KOiK02519.
OMAiKSNDHAD.
OrthoDBiEOG67HJSV.

Enzyme and pathway databases

BioCyciLCAS543734:GCHL-1763-MONOMER.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_B. IF_2_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERiPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Lactobacillus casei BL23 complete genome sequence."
    Maze A., Boel G., Bourand A., Loux V., Gibrat J.F., Zuniga M., Hartke A., Deutscher J.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BL23.

Entry informationi

Entry nameiIF2_LACCB
AccessioniPrimary (citable) accession number: B3WER5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 2, 2008
Last modified: April 1, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.