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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Lactobacillus casei (strain BL23)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathway: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi67 – 748ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLCAS543734:GCHL-1660-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:LCABL_16830
OrganismiLactobacillus casei (strain BL23)
Taxonomic identifieri543734 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 557557Formate--tetrahydrofolate ligasePRO_1000196810Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi543734.LCABL_16830.

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3WEG3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDIEIAQAN EATAMWPITK VAAGLGLSED DLELYGKAKA KLSFSALNAL
60 70 80 90 100
KDKPFGKLVL VTSINPTPAG EGKSTITVGL GDALQQRGKH PVIALREPSL
110 120 130 140 150
GPVMGMKGGA TGGGYAQVVP MEDINLHFTG DMHALTTAVD TLAALIDNHL
160 170 180 190 200
QQGNTLNIDP RRILWKRALD INDRALRHVT IGLGGPTSGV PREDGFDITV
210 220 230 240 250
ASELMAVLCL AENIADLKAR IGRIVIGYTY DRQPVTVADL KVTGAIAMLL
260 270 280 290 300
RDALKPNLVQ TLEHTPAFIH GGPFANIAHG CNSVLATRTA LQLGDIAITE
310 320 330 340 350
AGFGADLGGE KFMDIKTPVL GKTPDAVVIV ATVRALKYNG GVALKDLQTE
360 370 380 390 400
NVEALAQGFG NLKRHIHSMQ SYGVPVVVAI NRFTSDTDAE IQFLIDACAK
410 420 430 440 450
LNVKAVTATV WADGGRGGLA VADAVLAALD QPAHFTRLYD PQSDVKTKIK
460 470 480 490 500
TIVQKIYGGA DVDYEGKANS ALRTIVKNGW QDLPVCMAKT QYSLTDNAKT
510 520 530 540 550
LGAPEGFTIH VRDIIPKLGA GFLVVMTGSV LTMPGLPKVP AALNMDVTDD

GKISGLF
Length:557
Mass (Da):58,901
Last modified:September 2, 2008 - v1
Checksum:i70A7DE2024BAA25B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM177140 Genomic DNA. Translation: CAQ66764.1.
RefSeqiWP_003570898.1. NC_010999.1.
YP_001987622.1. NC_010999.1.

Genome annotation databases

EnsemblBacteriaiCAQ66764; CAQ66764; LCABL_16830.
KEGGilcb:LCABL_16830.
PATRICi22212805. VBILacCas89204_1589.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM177140 Genomic DNA. Translation: CAQ66764.1.
RefSeqiWP_003570898.1. NC_010999.1.
YP_001987622.1. NC_010999.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi543734.LCABL_16830.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAQ66764; CAQ66764; LCABL_16830.
KEGGilcb:LCABL_16830.
PATRICi22212805. VBILacCas89204_1589.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciLCAS543734:GCHL-1660-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Lactobacillus casei BL23 complete genome sequence."
    Maze A., Boel G., Bourand A., Loux V., Gibrat J.F., Zuniga M., Hartke A., Deutscher J.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BL23.

Entry informationi

Entry nameiFTHS_LACCB
AccessioniPrimary (citable) accession number: B3WEG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 2, 2008
Last modified: June 24, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.