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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Lactobacillus casei (strain BL23)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei578 – 5781ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLCAS543734:GCHL-930-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:LCABL_09490
OrganismiLactobacillus casei (strain BL23)
Taxonomic identifieri543734 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000001206: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 803803Leucine--tRNA ligasePRO_1000091325Add
BLAST

Proteomic databases

PRIDEiB3WCD5.

Interactioni

Protein-protein interaction databases

STRINGi543734.LCABL_09490.

Structurei

3D structure databases

ProteinModelPortaliB3WCD5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi575 – 5795"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3WCD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYDHHEIDK KWQRYWAEHN EFNTTTDPKK PNYYALDMFP YPSGQGLHVG
60 70 80 90 100
HPEGYTATDI VARMKRMQGF NVLHPMGWDA FGLPAEQYAL NTGHNPKTFT
110 120 130 140 150
KQNIETFKRQ INSLGFSYDW NREINTTDPN YYKWTQWIFE QLYKHGLAYE
160 170 180 190 200
AEVPVNWSPD LGTVVANEEV IDGKTERGGF PVVRKPMRQW MLKITAYAEK
210 220 230 240 250
LLTDLDDLDW PESIKQMQRN WIGKSTGAQI TFRVTDSHEP FDVFTTRPDT
260 270 280 290 300
LFGATYVVMA PEHELVQKIT TPAQQAVVDA YIDEAAHKSD LDRTALDKEK
310 320 330 340 350
TGVWTGAYAT NPVNGEKLPI WISDYVLASY GTGAIMSVPA HDDRDYAFAK
360 370 380 390 400
KFGIEIKPVI EGGNVDEAAY TGDGVHINSG FLDGLNEHDA IDRMIKWLED
410 420 430 440 450
KGIGSAKINY KLRDWVFSRQ RYWGEPIPVI HWEDGETTLV PEDELPLTLP
460 470 480 490 500
EEADIKPSGT GESPLANLTD WVNVVDKNGR KGKRETNTMP QWAGSSWYFL
510 520 530 540 550
RFVDPHNKEA LADYDKLKAW MPVDLYIGGA EHAVLHLLYA RFWNLFLYDI
560 570 580 590 600
GAIPNKEPFQ RLFNQGMILG DNHEKMSKSK GNVVNPDDVV DEYGADTLRL
610 620 630 640 650
YEMFMGPLDA GIAWSTKGLA GARKFLDRVW SAFIDDEGKL RDRITTINDG
660 670 680 690 700
RLDKVYNETV KKVTEDYDAL HFNTAISQMM VFINSARKDD DLPLEYVEGF
710 720 730 740 750
VKMLAPIAPH LMEEIWSRLG HDHSLTYAPW PSYDESKIKT DTYDMMIQVN
760 770 780 790 800
GKLRGSITAD VNESDDEIKQ AALANDNVQK FTAGKDIKKI IVVPRKIVNI

VAK
Length:803
Mass (Da):91,527
Last modified:September 2, 2008 - v1
Checksum:i487E45B7E7F7BA4B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM177140 Genomic DNA. Translation: CAQ66036.1.
RefSeqiYP_001986894.1. NC_010999.1.

Genome annotation databases

EnsemblBacteriaiCAQ66036; CAQ66036; LCABL_09490.
GeneIDi6404658.
KEGGilcb:LCABL_09490.
PATRICi22211365. VBILacCas89204_0876.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM177140 Genomic DNA. Translation: CAQ66036.1.
RefSeqiYP_001986894.1. NC_010999.1.

3D structure databases

ProteinModelPortaliB3WCD5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi543734.LCABL_09490.

Proteomic databases

PRIDEiB3WCD5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAQ66036; CAQ66036; LCABL_09490.
GeneIDi6404658.
KEGGilcb:LCABL_09490.
PATRICi22211365. VBILacCas89204_0876.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciLCAS543734:GCHL-930-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Lactobacillus casei BL23 complete genome sequence."
    Maze A., Boel G., Bourand A., Loux V., Gibrat J.F., Zuniga M., Hartke A., Deutscher J.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BL23.

Entry informationi

Entry nameiSYL_LACCB
AccessioniPrimary (citable) accession number: B3WCD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 2, 2008
Last modified: January 7, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.