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B3VQP5 (B3VQP5_9ENTO) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein names
OrganismHuman poliovirus 1 EMBL ACF16830.1
Taxonomic identifier12080 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesPicornaviridaeEnterovirusEnterovirus C

Protein attributes

Sequence length2209 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

NTP + H2O = NDP + phosphate. SAAS SAAS014759

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). SAAS SAAS014759

Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly. SAAS SAAS014759

Subcellular location

Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity SAAS SAAS014759.

Sequence similarities

Contains RdRp catalytic domain. SAAS SAAS014759

Contains SF3 helicase domain. SAAS SAAS014759

Contains SFhelicase domain. SAAS SAAS014759

Ontologies

Keywords
   Biological processHost-virus interaction
Ion transport
Transport
Viral attachment to host cell SAAS SAAS014759
Viral RNA replication SAAS SAAS014759
Virus entry into host cell
   Cellular componentCapsid protein SAAS SAAS014759
Host cytoplasm SAAS SAAS014759
Host cytoplasmic vesicle SAAS SAAS014759
Host membrane SAAS SAAS014759
Membrane
Virion
   LigandATP-binding
Manganese PDB 3OL8
Metal-binding PDB 3OL8 PDB 3OL9 PDB 3OLA PDB 3OLB PDB 3OL6
Nucleotide-binding
RNA-binding SAAS SAAS014759
Zinc PDB 3OL8 PDB 3OL9 PDB 3OLA PDB 3OLB PDB 3OL6
   Molecular functionHelicase SAAS SAAS014759
Hydrolase
Ion channel
Nucleotidyltransferase
Protease
RNA-directed RNA polymerase SAAS SAAS014759
Thiol protease SAAS SAAS014759
Transferase
Viral ion channel SAAS SAAS014759
   PTMPhosphoprotein SAAS SAAS014759
   Technical term3D-structure PDB 3OL8 PDB 3OL9 PDB 3OLA PDB 3OLB PDB 3OL6
Gene Ontology (GO)
   Biological_processRNA-protein covalent cross-linking

Inferred from electronic annotation. Source: InterPro

pore formation by virus in membrane of host cell

Inferred from electronic annotation. Source: UniProtKB-KW

protein oligomerization

Inferred from electronic annotation. Source: UniProtKB-KW

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: InterPro

viral RNA genome replication

Inferred from electronic annotation. Source: InterPro

viral entry into host cell

Inferred from electronic annotation. Source: UniProtKB-KW

virion attachment to host cell

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenthost cell cytoplasmic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane of host cell

Inferred from electronic annotation. Source: UniProtKB-KW

membrane

Inferred from electronic annotation. Source: UniProtKB-KW

viral capsid

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA helicase activity

Inferred from electronic annotation. Source: InterPro

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

ion channel activity

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

structural molecule activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6969capsid VP4 EMBL ACF16830.1
PRO_5000381452
Chain70 – 341272capsid VP2 EMBL ACF16830.1
PRO_5000381453
Chain342 – 579238capsid VP3 EMBL ACF16830.1
PRO_5000381454
Chain580 – 881302capsid VP1 EMBL ACF16830.1
PRO_5000381455
Chain882 – 1030149protease 2A EMBL ACF16830.1
PRO_5000381456
Chain1031 – 112797protein 2B EMBL ACF16830.1
PRO_5000381457
Chain1128 – 1456329ATPase 2C EMBL ACF16830.1
PRO_5000381458
Chain1457 – 154387protein 3A EMBL ACF16830.1
PRO_5000381459
Chain1544 – 156522protein 3B EMBL ACF16830.1
PRO_5000381460
Chain1566 – 1748183protease 3C EMBL ACF16830.1
PRO_5000381461
Chain1749 – 2209461polymerase 3D EMBL ACF16830.1
PRO_5000381462

Sites

Metal binding19811Manganese PDB 3OL8
Metal binding19821Manganese; via carbonyl oxygen PDB 3OL8
Metal binding20181Zinc 1; via tele nitrogen PDB 3OL8 PDB 3OL9 PDB 3OLA
Metal binding20201Zinc 1; via pros nitrogen PDB 3OL8 PDB 3OL9 PDB 3OLA
Metal binding20201Zinc 1; via tele nitrogen
Metal binding20201Zinc 2; via tele nitrogen
Metal binding20291Zinc 1 PDB 3OL8 PDB 3OL9 PDB 3OLA
Metal binding20291Zinc 2
Metal binding20761Manganese PDB 3OL8

Sequences

Sequence LengthMass (Da)Tools
B3VQP5 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: 600D35C305FA54F6

FASTA2,209246,506
        10         20         30         40         50         60 
MGAQVSSQKV GAHENSNRAY GGSTINYTTI NYYRDSASNA ASKQDFAQDP SKFTEPIKDV 

        70         80         90        100        110        120 
LIKTSPMLNS PNIEACGYSD RVLQLTLGNS TITTQEAANS VVAYGRWPEY LRDSEANPVD 

       130        140        150        160        170        180 
QPTEPDVAAC RFYTLDTVSW TKESRGWWWK LPDALRDMGL FGQNMYYHYL GRSGYTVHVQ 

       190        200        210        220        230        240 
CNASKFHQGA LGVFAVPEMC LAGDSNTTNI YTSYQNANPG ERGGTFTNTF TPDNNQESPA 

       250        260        270        280        290        300 
RKFCPVDYLF GNGTLLGNAF VFPHQIINLR TNNCATLVLP YVNSLSIDSM AKHNNWGIAI 

       310        320        330        340        350        360 
LPLAPLNFAS ESSPEIPITL TIAPMCCEFN GLRNITLPRL QGLPVMNTPG SNQYLTADNF 

       370        380        390        400        410        420 
QSPCALPEFD VTPPIDIPGE VKNMMELAEI DTMIPFDLSA TKKNTMEMYR VRLSDKPHTD 

       430        440        450        460        470        480 
DPILCLSLSP ASDPRLSHTM LGEILNYYTH WAGSLKFTFL FCGSMMATGK LLVSYAPPGA 

       490        500        510        520        530        540 
DPPKKRKEAM LGTHVIWDIG LQSSCTMVVP WISNTTYRQT IDDSFTEGGY ISVFYQTRIV 

       550        560        570        580        590        600 
VPLSTPREMD ILGFVSACND FSVRLLRDTT HIEQKALAQG LGQMLESMID NTVRETVGAA 

       610        620        630        640        650        660 
TSRDALPNTE ASGPAHSKEI PALTAVETGA TNPLVPSDTV QTRHVVQHRS RSESSIESFF 

       670        680        690        700        710        720 
ARGACVAILT VDNSASTKNN DKLFAVWKIT YKDTVQLRRK LEFFTYSRFD MEFTFVVTAN 

       730        740        750        760        770        780 
FTETNNGHAL NQVYQIMYVP PGAPVPGKWD DYTWQTSSNP SIFYTYGTAP ARISVPYVGI 

       790        800        810        820        830        840 
SNAYSHFYDG FSKIPLKDQS AALGDSLYGA ASLNDFGILA VRVVNDHNPT KVTSKIRVYL 

       850        860        870        880        890        900 
KPKHIRVWCP RPPRAVAYYG PGVDYKDGTL TPLPTKDLTT YGFGHQNKAV YTAGYKICNY 

       910        920        930        940        950        960 
HLATQEDLQN AVNVMWDRDL LVTESRAQGT DSIARCNCNA GVYYCESRRK YYPVSFVGPT 

       970        980        990       1000       1010       1020 
FQYMEANDYY PARYQSHMLI GHGFASPGDC GGILRCNHGV IGIITAGGEG LVAFSDIRDL 

      1030       1040       1050       1060       1070       1080 
YAYEEEAMEQ GITNYIESLG AAFGSGFTQQ IGDKITELTN MVTSTITEKL LKNLIKIISS 

      1090       1100       1110       1120       1130       1140 
LVIITRNYED TTTVLATLAL LGCDASPWQW LRKKACDILE IPYVIKQGDS WLKKFTEACN 

      1150       1160       1170       1180       1190       1200 
AAKGLEWVSN KISKFIDWLK EKIIPQARDK LEFVTKLKQL EMLENQISTI HQSCPSQEHQ 

      1210       1220       1230       1240       1250       1260 
EILFNNVRWL SIQSKRFAPL YAVEAKRIQK LEHTINNYIQ FKSKHRIEPV CLLVHGSPGT 

      1270       1280       1290       1300       1310       1320 
GKSVATNLIA RAIAERENTS TYSLPPDPSH FDGYKQQGVV IMDDLNQNPD GADMKLFCQM 

      1330       1340       1350       1360       1370       1380 
VSTVEFIPPM ASLEEKGILF TSNYVLASTN SSRISPPTVA HSDALARRFA FDMDIQVMNE 

      1390       1400       1410       1420       1430       1440 
YSRDGKLNMA MATEMCKNCH QPANFKRCCP LVCGKAIQLM DKSSRVRYSI DQITTMIINE 

      1450       1460       1470       1480       1490       1500 
RNRRSNIGNC MEALFQGPLQ YKDLKIDIKT SPPPECINDL LQAVDSQEVR DYCEKKGWIV 

      1510       1520       1530       1540       1550       1560 
NITSQVQTER NINRAMTILQ AVTTFAAVAG VVYVMYKLFA GHQGAYTGLP NKKPNVPTIR 

      1570       1580       1590       1600       1610       1620 
TAKVQGPGFD YAVAMAKRNI VTATTSKGEF TMLGVHDNVA ILPTHASPGE SIVIDGKEVE 

      1630       1640       1650       1660       1670       1680 
ILDAKALEDQ AGTNLEITII TLKRNEKFRD IRPHIPTQIT ETNDGVLIVN TNKYPNMYVP 

      1690       1700       1710       1720       1730       1740 
VGAVTEQGYL NLGGRQTART LMYNFPTRAG QCGGVITCTG KVIGMHVGGN GSHGFAAALK 

      1750       1760       1770       1780       1790       1800 
RSYFTQSQGE IQWMRPSKEV GYPIINAPSK TKLEPSAFHY VFEGVKEPAV LTKNDPRLKT 

      1810       1820       1830       1840       1850       1860 
DFEEAIFSKY VGNKITEVDE YMKEAVDHYA GQLMSLDINT EQMCLEDAMY GTDGLEALDL 

      1870       1880       1890       1900       1910       1920 
STSAGYPYVA MGKKKRDILN KQTRDTKEMQ KLLDTYGINL PLVTYVKDEL RSKTKVEQGK 

      1930       1940       1950       1960       1970       1980 
SRLIEASSLN DSVAMRMAFG NLYAAFHKNP GVITGSAVGC DPDLFWSKIP VLMEEKLFAF 

      1990       2000       2010       2020       2030       2040 
DYTGYDASLS PAWFEALKMV LEKIGFGDRV DYIDYLNHSH HLYKNKTYCV KGGMPSGCSG 

      2050       2060       2070       2080       2090       2100 
TSIFNSMINN LIIRTLLLKT YKGIDLDHLK MIAYGDDVIA SYPHEVDASL LAQSGKDYGL 

      2110       2120       2130       2140       2150       2160 
TMTPADKSAT FETVTWENVT FLKRFFRADE KYPFLIHPVM PMKEIHESIR WTKDPRNTQD 

      2170       2180       2190       2200 
HVRSLCLLAW HNGEEEYNKF LAKIRSVPIG RALLLPEYST LYRRWLDSF 

« Hide

References

[1]"Changes in population dynamics during long-term evolution of sabin type 1 poliovirus in an immunodeficient patient."
Odoom J.K., Yunus Z., Dunn G., Minor P.D., Martin J.
J. Virol. 82:9179-9190(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: A649 EMBL ACF16830.1.
[2]"Structural basis for active site closure by the poliovirus RNA-dependent RNA polymerase."
Gong P., Peersen O.B.
Proc. Natl. Acad. Sci. U.S.A. 107:22505-22510(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 1749-2209 IN COMPLEX WITH MANGANESE AND ZINC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EU794964 Genomic RNA. Translation: ACF16830.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3OL6X-ray2.50A/E/I/M1749-2209[»]
3OL8X-ray2.75A/E/I/M1749-2209[»]
3OL9X-ray2.25A/E/I/M1749-2209[»]
3OLAX-ray2.55A/E/I/M1749-2209[»]
3OLBX-ray2.41A/E/I/M1749-2209[»]
ProteinModelPortalB3VQP5.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D4.10.80.10. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR014838. P3A.
IPR000081. Peptidase_C3.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR003138. Pico_P1A.
IPR002527. Pico_P2B.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF08727. P3A. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF02226. Pico_P1A. 1 hit.
PF00947. Pico_P2A. 1 hit.
PF01552. Pico_P2B. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 3 hits.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
ProDomPD001306. Peptidase_C3. 1 hit.
PD649346. Pico_P2B. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF89043. SSF89043. 1 hit.
PROSITEPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceB3VQP5.

Entry information

Entry nameB3VQP5_9ENTO
AccessionPrimary (citable) accession number: B3VQP5
Entry history
Integrated into UniProtKB/TrEMBL: September 2, 2008
Last sequence update: September 2, 2008
Last modified: April 16, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)