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B3VQP5

- B3VQP5_9ENTO

UniProt

B3VQP5 - B3VQP5_9ENTO

Protein
Submitted name:

Polyprotein

Gene
N/A
Organism
Human poliovirus 1
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (02 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    NTP + H2O = NDP + phosphate.SAAS annotation
    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).SAAS annotation
    Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.SAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi1981 – 19811Manganese
    Metal bindingi1982 – 19821Manganese; via carbonyl oxygen
    Metal bindingi2018 – 20181Zinc 1; via tele nitrogen
    Metal bindingi2020 – 20201Zinc 1; via tele nitrogenImported
    Metal bindingi2020 – 20201Zinc 2; via pros nitrogenImported
    Metal bindingi2029 – 20291Zinc 1
    Metal bindingi2029 – 20291Zinc 2Imported
    Metal bindingi2076 – 20761Manganese

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. ion channel activity Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW
    5. RNA binding Source: UniProtKB-KW
    6. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    7. RNA helicase activity Source: InterPro
    8. structural molecule activity Source: InterPro

    GO - Biological processi

    1. pore formation by virus in membrane of host cell Source: UniProtKB-KW
    2. protein oligomerization Source: UniProtKB-KW
    3. RNA-protein covalent cross-linking Source: InterPro
    4. transcription, DNA-templated Source: InterPro
    5. viral entry into host cell Source: UniProtKB-KW
    6. viral RNA genome replication Source: InterPro
    7. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    HelicaseSAAS annotation, Hydrolase, Ion channel, Nucleotidyltransferase, Protease, RNA-directed RNA polymeraseSAAS annotation, Thiol proteaseSAAS annotation, Transferase, Viral ion channelSAAS annotation

    Keywords - Biological processi

    Host-virus interaction, Ion transport, Transport, Viral attachment to host cellSAAS annotation, Viral RNA replicationSAAS annotation, Virus entry into host cell

    Keywords - Ligandi

    ATP-binding, ManganeseImported, Metal-bindingImported, Nucleotide-binding, RNA-bindingSAAS annotation, ZincImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    PolyproteinImported
    OrganismiHuman poliovirus 1Imported
    Taxonomic identifieri12080 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesPicornaviridaeEnterovirusEnterovirus C

    Subcellular locationi

    Host cytoplasm SAAS annotation
    Host cytoplasmic vesicle membrane SAAS annotation; Peripheral membrane protein SAAS annotation; Cytoplasmic side SAAS annotation

    GO - Cellular componenti

    1. host cell cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    2. integral to membrane of host cell Source: UniProtKB-KW
    3. membrane Source: UniProtKB-KW
    4. viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid proteinSAAS annotation, Host cytoplasmSAAS annotation, Host cytoplasmic vesicleSAAS annotation, Host membraneSAAS annotation, Membrane, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6969capsid VP4ImportedPRO_5000381452Add
    BLAST
    Chaini70 – 341272capsid VP2ImportedPRO_5000381453Add
    BLAST
    Chaini342 – 579238capsid VP3ImportedPRO_5000381454Add
    BLAST
    Chaini580 – 881302capsid VP1ImportedPRO_5000381455Add
    BLAST
    Chaini882 – 1030149protease 2AImportedPRO_5000381456Add
    BLAST
    Chaini1031 – 112797protein 2BImportedPRO_5000381457Add
    BLAST
    Chaini1128 – 1456329ATPase 2CImportedPRO_5000381458Add
    BLAST
    Chaini1457 – 154387protein 3AImportedPRO_5000381459Add
    BLAST
    Chaini1544 – 156522protein 3BImportedPRO_5000381460Add
    BLAST
    Chaini1566 – 1748183protease 3CImportedPRO_5000381461Add
    BLAST
    Chaini1749 – 2209461polymerase 3DImportedPRO_5000381462Add
    BLAST

    Keywords - PTMi

    PhosphoproteinSAAS annotation

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3OL6X-ray2.50A/E/I/M1749-2209[»]
    3OL8X-ray2.75A/E/I/M1749-2209[»]
    3OL9X-ray2.25A/E/I/M1749-2209[»]
    3OLAX-ray2.55A/E/I/M1749-2209[»]
    3OLBX-ray2.41A/E/I/M1749-2209[»]
    ProteinModelPortaliB3VQP5.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiB3VQP5.

    Family & Domainsi

    Sequence similaritiesi

    Contains RdRp catalytic domain.SAAS annotation
    Contains SF3 helicase domain.SAAS annotation
    Contains SFhelicase domain.SAAS annotation

    Family and domain databases

    Gene3Di2.60.120.20. 3 hits.
    4.10.80.10. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR014838. P3A.
    IPR000081. Peptidase_C3.
    IPR000199. Peptidase_C3A/C3B_picornavir.
    IPR003138. Pico_P1A.
    IPR002527. Pico_P2B.
    IPR001676. Picornavirus_capsid.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view]
    PfamiPF08727. P3A. 1 hit.
    PF00548. Peptidase_C3. 1 hit.
    PF02226. Pico_P1A. 1 hit.
    PF00947. Pico_P2A. 1 hit.
    PF01552. Pico_P2B. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00073. Rhv. 3 hits.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view]
    ProDomiPD001306. Peptidase_C3. 1 hit.
    PD649346. Pico_P2B. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF89043. SSF89043. 1 hit.
    PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B3VQP5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGAQVSSQKV GAHENSNRAY GGSTINYTTI NYYRDSASNA ASKQDFAQDP     50
    SKFTEPIKDV LIKTSPMLNS PNIEACGYSD RVLQLTLGNS TITTQEAANS 100
    VVAYGRWPEY LRDSEANPVD QPTEPDVAAC RFYTLDTVSW TKESRGWWWK 150
    LPDALRDMGL FGQNMYYHYL GRSGYTVHVQ CNASKFHQGA LGVFAVPEMC 200
    LAGDSNTTNI YTSYQNANPG ERGGTFTNTF TPDNNQESPA RKFCPVDYLF 250
    GNGTLLGNAF VFPHQIINLR TNNCATLVLP YVNSLSIDSM AKHNNWGIAI 300
    LPLAPLNFAS ESSPEIPITL TIAPMCCEFN GLRNITLPRL QGLPVMNTPG 350
    SNQYLTADNF QSPCALPEFD VTPPIDIPGE VKNMMELAEI DTMIPFDLSA 400
    TKKNTMEMYR VRLSDKPHTD DPILCLSLSP ASDPRLSHTM LGEILNYYTH 450
    WAGSLKFTFL FCGSMMATGK LLVSYAPPGA DPPKKRKEAM LGTHVIWDIG 500
    LQSSCTMVVP WISNTTYRQT IDDSFTEGGY ISVFYQTRIV VPLSTPREMD 550
    ILGFVSACND FSVRLLRDTT HIEQKALAQG LGQMLESMID NTVRETVGAA 600
    TSRDALPNTE ASGPAHSKEI PALTAVETGA TNPLVPSDTV QTRHVVQHRS 650
    RSESSIESFF ARGACVAILT VDNSASTKNN DKLFAVWKIT YKDTVQLRRK 700
    LEFFTYSRFD MEFTFVVTAN FTETNNGHAL NQVYQIMYVP PGAPVPGKWD 750
    DYTWQTSSNP SIFYTYGTAP ARISVPYVGI SNAYSHFYDG FSKIPLKDQS 800
    AALGDSLYGA ASLNDFGILA VRVVNDHNPT KVTSKIRVYL KPKHIRVWCP 850
    RPPRAVAYYG PGVDYKDGTL TPLPTKDLTT YGFGHQNKAV YTAGYKICNY 900
    HLATQEDLQN AVNVMWDRDL LVTESRAQGT DSIARCNCNA GVYYCESRRK 950
    YYPVSFVGPT FQYMEANDYY PARYQSHMLI GHGFASPGDC GGILRCNHGV 1000
    IGIITAGGEG LVAFSDIRDL YAYEEEAMEQ GITNYIESLG AAFGSGFTQQ 1050
    IGDKITELTN MVTSTITEKL LKNLIKIISS LVIITRNYED TTTVLATLAL 1100
    LGCDASPWQW LRKKACDILE IPYVIKQGDS WLKKFTEACN AAKGLEWVSN 1150
    KISKFIDWLK EKIIPQARDK LEFVTKLKQL EMLENQISTI HQSCPSQEHQ 1200
    EILFNNVRWL SIQSKRFAPL YAVEAKRIQK LEHTINNYIQ FKSKHRIEPV 1250
    CLLVHGSPGT GKSVATNLIA RAIAERENTS TYSLPPDPSH FDGYKQQGVV 1300
    IMDDLNQNPD GADMKLFCQM VSTVEFIPPM ASLEEKGILF TSNYVLASTN 1350
    SSRISPPTVA HSDALARRFA FDMDIQVMNE YSRDGKLNMA MATEMCKNCH 1400
    QPANFKRCCP LVCGKAIQLM DKSSRVRYSI DQITTMIINE RNRRSNIGNC 1450
    MEALFQGPLQ YKDLKIDIKT SPPPECINDL LQAVDSQEVR DYCEKKGWIV 1500
    NITSQVQTER NINRAMTILQ AVTTFAAVAG VVYVMYKLFA GHQGAYTGLP 1550
    NKKPNVPTIR TAKVQGPGFD YAVAMAKRNI VTATTSKGEF TMLGVHDNVA 1600
    ILPTHASPGE SIVIDGKEVE ILDAKALEDQ AGTNLEITII TLKRNEKFRD 1650
    IRPHIPTQIT ETNDGVLIVN TNKYPNMYVP VGAVTEQGYL NLGGRQTART 1700
    LMYNFPTRAG QCGGVITCTG KVIGMHVGGN GSHGFAAALK RSYFTQSQGE 1750
    IQWMRPSKEV GYPIINAPSK TKLEPSAFHY VFEGVKEPAV LTKNDPRLKT 1800
    DFEEAIFSKY VGNKITEVDE YMKEAVDHYA GQLMSLDINT EQMCLEDAMY 1850
    GTDGLEALDL STSAGYPYVA MGKKKRDILN KQTRDTKEMQ KLLDTYGINL 1900
    PLVTYVKDEL RSKTKVEQGK SRLIEASSLN DSVAMRMAFG NLYAAFHKNP 1950
    GVITGSAVGC DPDLFWSKIP VLMEEKLFAF DYTGYDASLS PAWFEALKMV 2000
    LEKIGFGDRV DYIDYLNHSH HLYKNKTYCV KGGMPSGCSG TSIFNSMINN 2050
    LIIRTLLLKT YKGIDLDHLK MIAYGDDVIA SYPHEVDASL LAQSGKDYGL 2100
    TMTPADKSAT FETVTWENVT FLKRFFRADE KYPFLIHPVM PMKEIHESIR 2150
    WTKDPRNTQD HVRSLCLLAW HNGEEEYNKF LAKIRSVPIG RALLLPEYST 2200
    LYRRWLDSF 2209
    Length:2,209
    Mass (Da):246,506
    Last modified:September 2, 2008 - v1
    Checksum:i600D35C305FA54F6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU794964 Genomic RNA. Translation: ACF16830.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU794964 Genomic RNA. Translation: ACF16830.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3OL6 X-ray 2.50 A/E/I/M 1749-2209 [» ]
    3OL8 X-ray 2.75 A/E/I/M 1749-2209 [» ]
    3OL9 X-ray 2.25 A/E/I/M 1749-2209 [» ]
    3OLA X-ray 2.55 A/E/I/M 1749-2209 [» ]
    3OLB X-ray 2.41 A/E/I/M 1749-2209 [» ]
    ProteinModelPortali B3VQP5.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei B3VQP5.

    Family and domain databases

    Gene3Di 2.60.120.20. 3 hits.
    4.10.80.10. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR014838. P3A.
    IPR000081. Peptidase_C3.
    IPR000199. Peptidase_C3A/C3B_picornavir.
    IPR003138. Pico_P1A.
    IPR002527. Pico_P2B.
    IPR001676. Picornavirus_capsid.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view ]
    Pfami PF08727. P3A. 1 hit.
    PF00548. Peptidase_C3. 1 hit.
    PF02226. Pico_P1A. 1 hit.
    PF00947. Pico_P2A. 1 hit.
    PF01552. Pico_P2B. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00073. Rhv. 3 hits.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view ]
    ProDomi PD001306. Peptidase_C3. 1 hit.
    PD649346. Pico_P2B. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF89043. SSF89043. 1 hit.
    PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Changes in population dynamics during long-term evolution of sabin type 1 poliovirus in an immunodeficient patient."
      Odoom J.K., Yunus Z., Dunn G., Minor P.D., Martin J.
      J. Virol. 82:9179-9190(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: A649Imported.
    2. "Structural basis for active site closure by the poliovirus RNA-dependent RNA polymerase."
      Gong P., Peersen O.B.
      Proc. Natl. Acad. Sci. U.S.A. 107:22505-22510(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 1749-2209 IN COMPLEX WITH MANGANESE AND ZINC.

    Entry informationi

    Entry nameiB3VQP5_9ENTO
    AccessioniPrimary (citable) accession number: B3VQP5
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 2, 2008
    Last sequence update: September 2, 2008
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3