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Protein

Glutathione-specific gamma-glutamylcyclotransferase 1

Gene

Chac1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the cleavage glutathione into 5-oxoproline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei115Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Apoptosis, Neurogenesis, Notch signaling pathway, Unfolded protein response

Enzyme and pathway databases

ReactomeiR-RNO-174403. Glutathione synthesis and recycling.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione-specific gamma-glutamylcyclotransferase 1By similarity (EC:2.3.2.-By similarity)
Short name:
Gamma-GCG 1By similarity
Alternative name(s):
Blocks Notch proteinBy similarity
Short name:
BotchBy similarity
Cation transport regulator-like protein 1By similarity
Neuroprotective protein 71 Publication
Gene namesi
Name:Chac1By similarity
Synonyms:BotchBy similarity, Npg71 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1307153. Chac1.

Subcellular locationi

  • Cytoplasmcytosol By similarity
  • Golgi apparatustrans-Golgi network By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004179901 – 222Glutathione-specific gamma-glutamylcyclotransferase 1Add BLAST222

Proteomic databases

PaxDbiB3STU3.

PTM databases

iPTMnetiB3STU3.
PhosphoSitePlusiB3STU3.

Expressioni

Gene expression databases

BgeeiENSRNOG00000014387.
GenevisibleiB3STU3. RN.

Interactioni

Subunit structurei

Interacts with NOTCH1 (via extracellular region).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019392.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 40Substrate bindingBy similarity6

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3182. Eukaryota.
COG3703. LUCA.
GeneTreeiENSGT00390000003855.
HOGENOMiHOG000264264.
HOVERGENiHBG056733.
InParanoidiB3STU3.
KOiK07232.
OMAiPVPGTMK.
OrthoDBiEOG091G0MZM.
PhylomeDBiB3STU3.
TreeFamiTF313048.

Family and domain databases

CDDicd06661. GGCT_like. 1 hit.
Gene3Di3.10.490.10. 1 hit.
InterProiIPR006840. ChaC.
IPR013024. GGCT-like.
[Graphical view]
PANTHERiPTHR12192. PTHR12192. 1 hit.
PfamiPF04752. ChaC. 1 hit.
[Graphical view]
SUPFAMiSSF110857. SSF110857. 1 hit.

Sequencei

Sequence statusi: Complete.

B3STU3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQESAAQST PPPSLSPAPS AQPSWEDGDP QALWIFGYGS LVWKPDFAYS
60 70 80 90 100
DSRVGFVRGY SRRFWQGDTF HRGSDKMPGR VVTLLEDHEG CTWGVAYQVR
110 120 130 140 150
GEQVSEALKY LNVREAVLGG YDTKEVTFYP QDTPDQPLTA LAYVATPQNP
160 170 180 190 200
GYLGPAPEEV IATQILACRG FSGHNLEYLL RLADFMQLCG PQAQDEHLEA
210 220
IVDAVGSLLP CSYLSEQPLA LI
Length:222
Mass (Da):24,457
Last modified:September 2, 2008 - v1
Checksum:i3F6D2CDFA14BA3FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF688602 mRNA. Translation: ABX10438.1.
CH473949 Genomic DNA. Translation: EDL79903.1.
RefSeqiNP_001166908.1. NM_001173437.1.
UniGeneiRn.23367.

Genome annotation databases

EnsembliENSRNOT00000019392; ENSRNOP00000019392; ENSRNOG00000014387.
GeneIDi362196.
KEGGirno:362196.
UCSCiRGD:1307153. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF688602 mRNA. Translation: ABX10438.1.
CH473949 Genomic DNA. Translation: EDL79903.1.
RefSeqiNP_001166908.1. NM_001173437.1.
UniGeneiRn.23367.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019392.

PTM databases

iPTMnetiB3STU3.
PhosphoSitePlusiB3STU3.

Proteomic databases

PaxDbiB3STU3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019392; ENSRNOP00000019392; ENSRNOG00000014387.
GeneIDi362196.
KEGGirno:362196.
UCSCiRGD:1307153. rat.

Organism-specific databases

CTDi79094.
RGDi1307153. Chac1.

Phylogenomic databases

eggNOGiKOG3182. Eukaryota.
COG3703. LUCA.
GeneTreeiENSGT00390000003855.
HOGENOMiHOG000264264.
HOVERGENiHBG056733.
InParanoidiB3STU3.
KOiK07232.
OMAiPVPGTMK.
OrthoDBiEOG091G0MZM.
PhylomeDBiB3STU3.
TreeFamiTF313048.

Enzyme and pathway databases

ReactomeiR-RNO-174403. Glutathione synthesis and recycling.

Miscellaneous databases

PROiB3STU3.

Gene expression databases

BgeeiENSRNOG00000014387.
GenevisibleiB3STU3. RN.

Family and domain databases

CDDicd06661. GGCT_like. 1 hit.
Gene3Di3.10.490.10. 1 hit.
InterProiIPR006840. ChaC.
IPR013024. GGCT-like.
[Graphical view]
PANTHERiPTHR12192. PTHR12192. 1 hit.
PfamiPF04752. ChaC. 1 hit.
[Graphical view]
SUPFAMiSSF110857. SSF110857. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCHAC1_RAT
AccessioniPrimary (citable) accession number: B3STU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: September 2, 2008
Last modified: November 2, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.