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B3SRS1

- NSP3_ROTAD

UniProt

B3SRS1 - NSP3_ROTAD

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Protein

Non-structural protein 3

Gene
N/A
Organism
Rotavirus A (strain Human/United States/D/1974 G1-P1A[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 3
Short name:
NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name:
NS34
OrganismiRotavirus A (strain Human/United States/D/1974 G1-P1A[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A)
Taxonomic identifieri578831 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000006368: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Non-structural protein 3PRO_0000369454Add
BLAST

Interactioni

Subunit structurei

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 146146RNA-binding By similarityAdd
BLAST
Regioni147 – 20357Dimerization By similarityAdd
BLAST
Regioni167 – 23165Interaction with ZC3H7B By similarityAdd
BLAST
Regioni205 – 310106Interaction with EIF4G1 By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili163 – 23472 Reviewed predictionAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus A NSP3 family.

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamiPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMiSSF69903. SSF69903. 1 hit.

Sequencei

Sequence statusi: Complete.

B3SRS1-1 [UniParc]FASTAAdd to Basket

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MESTQQMVSS IINTSFEAAV VAATSTLELM GIQYDYNEVF TRVKSKFDYV    50
MDDSGVKNNL LGKAITIDQA LNGKFGSAIR NRNWMTDSKT VAKLDEDVNK 100
LRMTLSSKGI DQKMRVLNAC FSVKRIPGKS SSIIKCTRLM KDKIERGEVE 150
VDDSYVDEKM EIDTIDWKSR YDQLEKRFEA LKQRVNEKYN TWVQKAKKVN 200
ENMYSLQNVI SQQQNQIADL QQYCNKLEAD LQGKFSSLVS SVEWYLRSME 250
LPDDVKNDIE QQLNSIDLIN PINAIDDIES LIRNLIQDYD RTFLMLKGLL 300
KQCNYEYACE 310
Length:310
Mass (Da):35,787
Last modified:September 2, 2008 - v1
Checksum:iE1993767AB0A61FE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF672572 Genomic RNA. Translation: ABV53246.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF672572 Genomic RNA. Translation: ABV53246.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002873. Rotavirus_NSP3.
[Graphical view ]
Pfami PF01665. Rota_NSP3. 1 hit.
[Graphical view ]
SUPFAMi SSF69903. SSF69903. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
    Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
    J. Virol. 82:11106-11116(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNSP3_ROTAD
AccessioniPrimary (citable) accession number: B3SRS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 2, 2008
Last modified: July 9, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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