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B3SRR3 (NSP3_ROTH7) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-structural protein 3

Short name=NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name=NS34
OrganismRotavirus A (isolate Human/United Kingdom/A64/1987 G10-P11[14]-I2-R2-C2-M1-A3-N2-T6-E2-H3) (RV-A) [Complete proteome]
Taxonomic identifier578827 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length310 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.

Subunit structure

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.

Sequence similarities

Belongs to the rotavirus A NSP3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 310310Non-structural protein 3
PRO_0000369443

Regions

Region1 – 146146RNA-binding By similarity
Region147 – 20357Dimerization By similarity
Region167 – 23165Interaction with ZC3H7B By similarity
Region205 – 310106Interaction with EIF4G1 By similarity
Coiled coil163 – 23472 Potential

Sequences

Sequence LengthMass (Da)Tools
B3SRR3 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: 1CDB0F8D02C90349

FASTA31035,973
        10         20         30         40         50         60 
MESTQQMASS IINTSFEAAV VAATSTLELM GIQYDYNEVY TRVKSKFDYI MDDSGVKNNL 

        70         80         90        100        110        120 
LGKAATIDQA LNGKFGSAVR NRNWMADTRT TARLDEDVNK LRMMLSSKGI DQKMRVLNAC 

       130        140        150        160        170        180 
FSVKRVPGKS SSIIKCTRLM RDKIERGEVE VDDSFVEEKM EVDTIDWKSR YEQLEKRFES 

       190        200        210        220        230        240 
LKQRVNEKYT SWVQKAKKVN ENMYSLQNVI SQQQSQIADL QHYCNKLEVD LQNKISSLVS 

       250        260        270        280        290        300 
SVEWYMKSME LPGEIKTDIE QQLNSIDVIN PINAIDDFES LIRNIILDYD RIFLMFKGLM 

       310 
RQCNYEYTYE 

« Hide

References

[1]"Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
J. Virol. 82:11106-11116(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF672565 Genomic RNA. Translation: ABV53238.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMSSF69903. SSF69903. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNSP3_ROTH7
AccessionPrimary (citable) accession number: B3SRR3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 2, 2008
Last modified: October 16, 2013
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families