SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B3SRR3

- NSP3_ROTH7

UniProt

B3SRR3 - NSP3_ROTH7

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Non-structural protein 3
Gene
N/A
Organism
Rotavirus A (isolate Human/United Kingdom/A64/1987 G10-P11[14]-I2-R2-C2-M1-A3-N2-T6-E2-H3) (RV-A)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Involved in the shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. suppression by virus of host translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 3
Short name:
NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name:
NS34
OrganismiRotavirus A (isolate Human/United Kingdom/A64/1987 G10-P11[14]-I2-R2-C2-M1-A3-N2-T6-E2-H3) (RV-A)
Taxonomic identifieri578827 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000001456: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Non-structural protein 3
PRO_0000369443Add
BLAST

Interactioni

Subunit structurei

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 146146RNA-binding By similarity
Add
BLAST
Regioni147 – 20357Dimerization By similarity
Add
BLAST
Regioni167 – 23165Interaction with ZC3H7B By similarity
Add
BLAST
Regioni205 – 310106Interaction with EIF4G1 By similarity
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili163 – 23472 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the rotavirus A NSP3 family.

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamiPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMiSSF69903. SSF69903. 1 hit.

Sequencei

Sequence statusi: Complete.

B3SRR3-1 [UniParc]FASTAAdd to Basket

« Hide

MESTQQMASS IINTSFEAAV VAATSTLELM GIQYDYNEVY TRVKSKFDYI    50
MDDSGVKNNL LGKAATIDQA LNGKFGSAVR NRNWMADTRT TARLDEDVNK 100
LRMMLSSKGI DQKMRVLNAC FSVKRVPGKS SSIIKCTRLM RDKIERGEVE 150
VDDSFVEEKM EVDTIDWKSR YEQLEKRFES LKQRVNEKYT SWVQKAKKVN 200
ENMYSLQNVI SQQQSQIADL QHYCNKLEVD LQNKISSLVS SVEWYMKSME 250
LPGEIKTDIE QQLNSIDVIN PINAIDDFES LIRNIILDYD RIFLMFKGLM 300
RQCNYEYTYE 310
Length:310
Mass (Da):35,973
Last modified:September 2, 2008 - v1
Checksum:i1CDB0F8D02C90349
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF672565 Genomic RNA. Translation: ABV53238.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF672565 Genomic RNA. Translation: ABV53238.1 .

3D structure databases

ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002873. Rotavirus_NSP3.
[Graphical view ]
Pfami PF01665. Rota_NSP3. 1 hit.
[Graphical view ]
SUPFAMi SSF69903. SSF69903. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
    Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
    J. Virol. 82:11106-11116(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNSP3_ROTH7
AccessioniPrimary (citable) accession number: B3SRR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 2, 2008
Last modified: July 9, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi