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Protein

Non-structural protein 3

Gene
N/A
Organism
Rotavirus A (isolate Human/United Kingdom/A64/1987 G10-P11[14]-I2-R2-C2-M1-A3-N2-T6-E2-H3) (RV-A)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Involved in the shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection (By similarity).By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. regulation of translation Source: UniProtKB-KW
  2. suppression by virus of host gene expression Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 3
Short name:
NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name:
NS34
OrganismiRotavirus A (isolate Human/United Kingdom/A64/1987 G10-P11[14]-I2-R2-C2-M1-A3-N2-T6-E2-H3) (RV-A)
Taxonomic identifieri578827 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000001456 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Non-structural protein 3PRO_0000369443Add
BLAST

Interactioni

Subunit structurei

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 (By similarity).By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 146146RNA-bindingBy similarityAdd
BLAST
Regioni147 – 20357DimerizationBy similarityAdd
BLAST
Regioni167 – 23165Interaction with ZC3H7BBy similarityAdd
BLAST
Regioni205 – 310106Interaction with EIF4G1By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili163 – 23472Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus A NSP3 family.Curated

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamiPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMiSSF69903. SSF69903. 1 hit.

Sequencei

Sequence statusi: Complete.

B3SRR3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESTQQMASS IINTSFEAAV VAATSTLELM GIQYDYNEVY TRVKSKFDYI
60 70 80 90 100
MDDSGVKNNL LGKAATIDQA LNGKFGSAVR NRNWMADTRT TARLDEDVNK
110 120 130 140 150
LRMMLSSKGI DQKMRVLNAC FSVKRVPGKS SSIIKCTRLM RDKIERGEVE
160 170 180 190 200
VDDSFVEEKM EVDTIDWKSR YEQLEKRFES LKQRVNEKYT SWVQKAKKVN
210 220 230 240 250
ENMYSLQNVI SQQQSQIADL QHYCNKLEVD LQNKISSLVS SVEWYMKSME
260 270 280 290 300
LPGEIKTDIE QQLNSIDVIN PINAIDDFES LIRNIILDYD RIFLMFKGLM
310
RQCNYEYTYE
Length:310
Mass (Da):35,973
Last modified:September 2, 2008 - v1
Checksum:i1CDB0F8D02C90349
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF672565 Genomic RNA. Translation: ABV53238.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF672565 Genomic RNA. Translation: ABV53238.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamiPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMiSSF69903. SSF69903. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
    Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
    J. Virol. 82:11106-11116(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNSP3_ROTH7
AccessioniPrimary (citable) accession number: B3SRR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 2, 2008
Last modified: January 7, 2015
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.