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B3SRQ6

- NSP2_ROTH6

UniProt

B3SRQ6 - NSP2_ROTH6

Protein

Non-structural protein 2

Gene
N/A
Organism
Rotavirus A (strain Human/Indonesia/69M/1980 G8-P4[10]-I2-R2-C2-M2-A2-N2-T2-E2-H2) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 24 (01 Oct 2014)
      Sequence version 1 (02 Sep 2008)
      Previous versions | rss
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    Functioni

    Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments By similarity.By similarity

    Cofactori

    Magnesium for NTPase activity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi153 – 1531MagnesiumSequence Analysis
    Metal bindingi171 – 1711MagnesiumSequence Analysis
    Active sitei225 – 2251For NTPase activityBy similarity

    GO - Molecular functioni

    1. hydrolase activity, acting on acid anhydrides Source: InterPro
    2. metal ion binding Source: UniProtKB-KW
    3. nucleotide binding Source: UniProtKB-KW
    4. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. viral genome replication Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-structural protein 2 (EC:3.6.4.-)
    Short name:
    NSP2
    Alternative name(s):
    NCVP3
    Non-structural RNA-binding protein 35
    Short name:
    NS35
    OrganismiRotavirus A (strain Human/Indonesia/69M/1980 G8-P4[10]-I2-R2-C2-M2-A2-N2-T2-E2-H2) (RV-A)
    Taxonomic identifieri10947 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000001455: Genome

    Subcellular locationi

    Host cytoplasm Curated
    Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 317317Non-structural protein 2PRO_0000369534Add
    BLAST

    Interactioni

    Subunit structurei

    Homooctamer By similarity. Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories By similarity.By similarity

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni205 – 24137RNA-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the rotavirus NSP2 family.Curated

    Family and domain databases

    Gene3Di3.30.428.20. 1 hit.
    3.90.1400.10. 1 hit.
    InterProiIPR003668. Rotavirus_NSP2.
    IPR024076. Rotavirus_NSP2_C-term.
    IPR024068. Rotavirus_NSP2_N.
    [Graphical view]
    PfamiPF02509. Rota_NS35. 1 hit.
    [Graphical view]
    SUPFAMiSSF75347. SSF75347. 1 hit.
    SSF75574. SSF75574. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B3SRQ6-1 [UniParc]FASTAAdd to Basket

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    MAELACFCYP HLENDSYKFI PFNSLAIKCM LTAKVDKKDQ DKFYNSIIYG    50
    IAPPPQFKKR YNTNDNSRGM NYETSMFNKV AMLVCEALNS IKVTQSDVAS 100
    VLSKVVSVRH LENLVLRREN HQDVLFHSKE LLLKSVLIAV GHSKEIETTA 150
    TAEGGEIVFQ NAAFTMWRLT YLEHKLMPIL DQNFIEYKIT VNEDKPVLES 200
    HVKELIAELR WQYNKFAVIT HGKGHYRVVK YSSVANHADR VYATFKSNNK 250
    NGNMLEFNLL DQRIIWQNWY AFTSSMKQGN TLDVCKRLLF QKMKRESNPF 300
    KGLSTDRKMD EVSQVGI 317
    Length:317
    Mass (Da):36,657
    Last modified:September 2, 2008 - v1
    Checksum:iFF5C93561D49A468
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF672559 Genomic DNA. Translation: ABV53231.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF672559 Genomic DNA. Translation: ABV53231.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.30.428.20. 1 hit.
    3.90.1400.10. 1 hit.
    InterProi IPR003668. Rotavirus_NSP2.
    IPR024076. Rotavirus_NSP2_C-term.
    IPR024068. Rotavirus_NSP2_N.
    [Graphical view ]
    Pfami PF02509. Rota_NS35. 1 hit.
    [Graphical view ]
    SUPFAMi SSF75347. SSF75347. 1 hit.
    SSF75574. SSF75574. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
      Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
      J. Virol. 82:11106-11116(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiNSP2_ROTH6
    AccessioniPrimary (citable) accession number: B3SRQ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: September 2, 2008
    Last modified: October 1, 2014
    This is version 24 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3