B3RAU1 (B3RAU1_CUPTR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172
EC=2.1.1.14 HAMAP MF_00172

Alternative name(s):
Cobalamin-independent methionine synthase HAMAP MF_00172
Methionine synthase, vitamin-B12 independent isozyme HAMAP MF_00172

Gene names

Name:	metE HAMAP MF_00172 EMBL CAQ72016.1
Ordered Locus Names:	RALTA_B1420

Organism

Cupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424)) [Complete proteome] [HAMAP]

Taxonomic identifier

164546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	796 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172 SAAS SAAS013215
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172 SAAS SAAS013215
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172 SAAS SAAS013215
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family. HAMAP MF_00172

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis HAMAP MF_00172 SAAS SAAS013215
Domain	Repeat HAMAP MF_00172
Ligand	Metal-binding HAMAP MF_00172 SAAS SAAS013215 Zinc HAMAP MF_00172 SAAS SAAS013215
Molecular function	Methyltransferase HAMAP MF_00172 SAAS SAAS013215 EMBL CAQ72016.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	646	1	Zinc By similarity HAMAP MF_00172
	Metal binding	648	1	Zinc By similarity HAMAP MF_00172
	Metal binding	731	1	Zinc By similarity HAMAP MF_00172

Sequences

	Sequence		Length	Mass (Da)
	B3RAU1 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: F603696953F83E0E	FASTA	796	88,728
	10 20 30 40 50 60 MARTHILGFP RIGERRELKF AQEAFWRGER TEAELRTVAA QLRRRHWQLQ AERGLDTVAT 70 80 90 100 110 120 GDFAWYDQML SLTALLGALP RRFGFDPAQL TLAQYFELAR GNREQPAMEM TKWFDTNYHY 130 140 150 160 170 180 LVPELDADTT FDGGPQWFFE ETDEALALGL RARPALIGPV TYLWLSKSHV PGFDRLSLLP 190 200 210 220 230 240 RLLQAYRRIL GQLQARGIEW VQIDEPALCL DLEPAWLDAF DTAYAALQED GPTARPKLLL 250 260 270 280 290 300 ATYFDTAADH AQRACALPVD GFHIDLVRAP AQLAAWQAAL PAHAVLSLGV IDGRNIWRTD 310 320 330 340 350 360 LRRVLDLLRP LHGKLGERLW LAPSCSLLHV PVSLAQESRL DAELKSWLAF ATEKLDELSV 370 380 390 400 410 420 LARALNQGDG AVADALSASD AAQASRRASR RVVNPRVQQR LAGVNDAMAN RASPFAERIE 430 440 450 460 470 480 HQRKALALPL LPTTTIGSFP QTAAIRQTRA AFKRGEIGAL EYLERIRAEI ALAVRKQEAL 490 500 510 520 530 540 GLDVLVHGEA ERNDMVEYFG EQLCGYGFTE NGWVQSYGSR CVKPPVIYGD VYRPEPMTVD 550 560 570 580 590 600 TARYAQSLTE RPMKGMLTGP ITMLQWSFVR DDQPRATTAR QLALAIRDEV CDLEQAGIRV 610 620 630 640 650 660 IQIDEPALRE GLPLRRADWD AYLDWAVTAF RLSASGVRDQ TQIHTHMCYA EFNDILPAIA 670 680 690 700 710 720 AMDADVITIE TSRSAMELLE GFGDFEYPNE IGPGVYDIHS PRVPSVQAME RLLERACEVV 730 740 750 760 770 780 PPQRLWVNPD CGLKTRGWEE TEAALANMVS AARALRQRLS APEVATWKRV TRTATTGAAP 790 VPHAAGACTA CATHAH

References

[1]

"Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia." Amadou C., Pascal G., Mangenot S., Glew M., Bontemps C., Capela D., Carrere S., Cruveiller S., Dossat C., Lajus A., Marchetti M., Poinsot V., Rouy Z., Servin B., Saad M., Schenowitz C., Barbe V., Batut J., Medigue C., Masson-Boivin C. Genome Res. 18:1472-1483(2008) [PubMed: 18490699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU633750 Genomic DNA. Translation: CAQ72016.1.
RefSeq	YP_002008072.1. NC_010530.1.
3D structure databases
ProteinModelPortal	B3RAU1.
ModBase	Search...
Protein-protein interaction databases
STRING	B3RAU1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6456832.
GenomeReviews	Gene locus RALTA_B1420 in contig CU633750_GR.
KEGG	cti:RALTA_B1420.
PATRIC	21536513. VBICupTai42494_4866.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	LWLSKSH.
ProtClustDB	PRK05222.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B3RAU1_CUPTR

Accession

Primary (citable) accession number: B3RAU1

Entry history

Integrated into UniProtKB/TrEMBL:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	December 14, 2011
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)