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Protein

Probable L-aspartate dehydrogenase

Gene

nadX

Organism
Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CIP 107171 / LMG 19424 / R1) (Ralstonia taiwanensis (strain LMG 19424))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate.UniRule annotation

Miscellaneous

The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia.UniRule annotation

Catalytic activityi

L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes iminoaspartate from L-aspartate (dehydrogenase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Probable L-aspartate dehydrogenase (nadX)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes iminoaspartate from L-aspartate (dehydrogenase route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei123NAD; via amide nitrogenUniRule annotation1
Binding sitei189NADUniRule annotation1
Active sitei219UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPyridine nucleotide biosynthesis
LigandNAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00253; UER00456.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable L-aspartate dehydrogenaseUniRule annotation (EC:1.4.1.21UniRule annotation)
Gene namesi
Name:nadXUniRule annotation
Ordered Locus Names:RALTA_B0572
OrganismiCupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CIP 107171 / LMG 19424 / R1) (Ralstonia taiwanensis (strain LMG 19424))
Taxonomic identifieri977880 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000001692 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001400861 – 266Probable L-aspartate dehydrogenaseAdd BLAST266

Interactioni

Protein-protein interaction databases

STRINGi977880.RALTA_B0572.

Structurei

3D structure databases

ProteinModelPortaliB3R8S4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the L-aspartate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108Q27. Bacteria.
COG1712. LUCA.
HOGENOMiHOG000206326.
KOiK06989.

Family and domain databases

HAMAPiMF_01265. NadX. 1 hit.
InterProiView protein in InterPro
IPR005106. Asp/hSer_DH_NAD-bd.
IPR002811. Asp_DH.
IPR020626. Asp_DH_prok.
IPR011182. L-Asp_DH.
IPR016040. NAD(P)-bd_dom.
PfamiView protein in Pfam
PF01958. DUF108. 1 hit.
PF03447. NAD_binding_3. 1 hit.
PIRSFiPIRSF005227. Asp_dh_NAD_syn. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

B3R8S4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHVSMVGCG AIGRGVLELL KSDPDVVFDV VIVPEHTMDE ARGAVSALAP
60 70 80 90 100
RARVATHLDD QRPDLLVECA GHHALEEHIV PALERGIPCM VVSVGALSEP
110 120 130 140 150
GMAERLEAAA RRGGTQVQLL SGAIGAIDAL AAARVGGLDE VIYTGRKPAR
160 170 180 190 200
AWTGTPAEQL FDLEALTEAT VIFEGTARDA ARLYPKNANV AATVSLAGLG
210 220 230 240 250
LDRTAVKLLA DPHAVENVHH VEARGAFGGF ELTMRGKPLA ANPKTSALTV
260
FSVVRALGNR AHAVSI
Length:266
Mass (Da):27,799
Last modified:September 2, 2008 - v1
Checksum:iACE91AE7E3C72B96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU633750 Genomic DNA. Translation: CAQ71192.1.
RefSeqiWP_012355417.1. NC_010530.1.

Genome annotation databases

EnsemblBacteriaiCAQ71192; CAQ71192; RALTA_B0572.
GeneIDi29763879.
KEGGicti:RALTA_B0572.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiASPD_CUPTR
AccessioniPrimary (citable) accession number: B3R8S4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 2, 2008
Last modified: June 7, 2017
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families