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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CIP 107171 / LMG 19424 / R1) (Ralstonia taiwanensis (strain LMG 19424))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi51Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi55Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi83Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei85Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei152Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei177Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei181Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei375Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei379Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei485Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei534Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei561Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei797Substrate; via amide nitrogenUniRule annotation1
Binding sitei805Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei822Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciCTAI977880:G1GJV-3796-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:RALTA_B0651
OrganismiCupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CIP 107171 / LMG 19424 / R1) (Ralstonia taiwanensis (strain LMG 19424))
Taxonomic identifieri977880 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000001692 Componenti: Chromosome 2

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Tat-type signalUniRule annotationAdd BLAST29
ChainiPRO_100018635230 – 831Periplasmic nitrate reductaseUniRule annotationAdd BLAST802

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi977880.RALTA_B0651.

Structurei

3D structure databases

ProteinModelPortaliB3R8B4.
SMRiB3R8B4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 974Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni214 – 221Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni245 – 249Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni264 – 266Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni511 – 512Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni721 – 730Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW. Bacteria.
COG0243. LUCA.
HOGENOMiHOG000031441.
KOiK02567.
OMAiTQHWRQQ.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct_NapA. 1 hit.
InterProiView protein in InterPro
IPR009010. Asp_de-COase-like_dom_sf.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
PANTHERiPTHR11615:SF123. PTHR11615:SF123. 1 hit.
PfamiView protein in Pfam
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
SMARTiView protein in SMART
SM00926. Molybdop_Fe4S4. 1 hit.
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiView protein in PROSITE
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B3R8B4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLSRRDFIK QTAVAATASV AGVTLPAGAA NFVTDSEVTK LKWSKAPCRF
60 70 80 90 100
CGTGCGVTVA VRDNKVVATN GDPQAEVNKG LNCVKGYFLS KIMYGQDRLT
110 120 130 140 150
KPLLRMKNGQ YDKNGEFAPV TWERAFDEME RQFKRVLKEK GPTAVGMFGS
160 170 180 190 200
GQWTVWEGYA ASKLYKAGFR SNNIDPNARH CMASAVQGFM RTFGMDEPMG
210 220 230 240 250
CYDDFEAADA FVLWGSNMAE MHPILWTRIT DRRLSHPKTR VAVLSTFTHR
260 270 280 290 300
SFDLADIPVI FKPQTDLAMM NYIAHYIIKN NKVNKDFVNK HTVFKEGVTN
310 320 330 340 350
IGYGLRPDHP LQKAAKNAAD PGASRPITFD DFARFVAKYD ADTVSKLSGV
360 370 380 390 400
PKDKLDQLAE LYADTNIKVM SLWTMGFNQH TRGSWANNMV YNLHLLTGKI
410 420 430 440 450
ATPGNSPFSL TGQPSACGTA REVGTFSHRL PADMVVTNPK HREEAERIWK
460 470 480 490 500
LPPGTIPDKP GYHAVLQNRM LKDGKLNAYW VQVNNNMQAA ANLMEEGLPG
510 520 530 540 550
YRNPANFIVV SDAYPTVTAL AADLILPSAM WVEKEGAYGN AERRTQFWHQ
560 570 580 590 600
LVDAPGEARS DLWQLMEFSK RFKVEDVWPA DLIAKKPEYR GKTLFDVLYR
610 620 630 640 650
NGQVDKFPLK EVNAEYHNAE AKAFGFYVQK GLFEEYATFG RGHGHDLAPF
660 670 680 690 700
DAYHEARGLR WPVVNGKETR WRYREGSDPY VKAGTGYQFY GNPDGKAVIF
710 720 730 740 750
ALPYEPPAES PDKEYPYWLV TGRVLEHWHS GSMTRRVPEL YRAFPNAVVF
760 770 780 790 800
MHPEDAKAMG LRRGVEVEVV SRRGSMRSRL ETRGRDAPPR GLVFVPWFDA
810 820 830
SQLINKVTLD ATCPISLQTD FKKCAVKIVK V
Length:831
Mass (Da):93,381
Last modified:September 2, 2008 - v1
Checksum:i09339B5B92523DD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU633750 Genomic DNA. Translation: CAQ71270.1.
RefSeqiWP_012355493.1. NC_010530.1.

Genome annotation databases

EnsemblBacteriaiCAQ71270; CAQ71270; RALTA_B0651.
GeneIDi29765796.
KEGGicti:RALTA_B0651.

Similar proteinsi

Entry informationi

Entry nameiNAPA_CUPTR
AccessioniPrimary (citable) accession number: B3R8B4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 2, 2008
Last modified: March 28, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome