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B3R8B4 (NAPA_CUPTR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:RALTA_B0651
OrganismCupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424)) [Complete proteome] [HAMAP]
Taxonomic identifier164546 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length831 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929Tat-type signal Potential
Chain30 – 831802Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000186352

Regions

Domain41 – 97574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding551Iron-sulfur (4Fe-4S) By similarity
Metal binding831Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B3R8B4 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: 09339B5B92523DD3

FASTA83193,381
        10         20         30         40         50         60 
MTLSRRDFIK QTAVAATASV AGVTLPAGAA NFVTDSEVTK LKWSKAPCRF CGTGCGVTVA 

        70         80         90        100        110        120 
VRDNKVVATN GDPQAEVNKG LNCVKGYFLS KIMYGQDRLT KPLLRMKNGQ YDKNGEFAPV 

       130        140        150        160        170        180 
TWERAFDEME RQFKRVLKEK GPTAVGMFGS GQWTVWEGYA ASKLYKAGFR SNNIDPNARH 

       190        200        210        220        230        240 
CMASAVQGFM RTFGMDEPMG CYDDFEAADA FVLWGSNMAE MHPILWTRIT DRRLSHPKTR 

       250        260        270        280        290        300 
VAVLSTFTHR SFDLADIPVI FKPQTDLAMM NYIAHYIIKN NKVNKDFVNK HTVFKEGVTN 

       310        320        330        340        350        360 
IGYGLRPDHP LQKAAKNAAD PGASRPITFD DFARFVAKYD ADTVSKLSGV PKDKLDQLAE 

       370        380        390        400        410        420 
LYADTNIKVM SLWTMGFNQH TRGSWANNMV YNLHLLTGKI ATPGNSPFSL TGQPSACGTA 

       430        440        450        460        470        480 
REVGTFSHRL PADMVVTNPK HREEAERIWK LPPGTIPDKP GYHAVLQNRM LKDGKLNAYW 

       490        500        510        520        530        540 
VQVNNNMQAA ANLMEEGLPG YRNPANFIVV SDAYPTVTAL AADLILPSAM WVEKEGAYGN 

       550        560        570        580        590        600 
AERRTQFWHQ LVDAPGEARS DLWQLMEFSK RFKVEDVWPA DLIAKKPEYR GKTLFDVLYR 

       610        620        630        640        650        660 
NGQVDKFPLK EVNAEYHNAE AKAFGFYVQK GLFEEYATFG RGHGHDLAPF DAYHEARGLR 

       670        680        690        700        710        720 
WPVVNGKETR WRYREGSDPY VKAGTGYQFY GNPDGKAVIF ALPYEPPAES PDKEYPYWLV 

       730        740        750        760        770        780 
TGRVLEHWHS GSMTRRVPEL YRAFPNAVVF MHPEDAKAMG LRRGVEVEVV SRRGSMRSRL 

       790        800        810        820        830 
ETRGRDAPPR GLVFVPWFDA SQLINKVTLD ATCPISLQTD FKKCAVKIVK V 

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References

[1]"Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia."
Amadou C., Pascal G., Mangenot S., Glew M., Bontemps C., Capela D., Carrere S., Cruveiller S., Dossat C., Lajus A., Marchetti M., Poinsot V., Rouy Z., Servin B., Saad M., Schenowitz C., Barbe V., Batut J., Medigue C., Masson-Boivin C.
Genome Res. 18:1472-1483(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: R1 / LMG 19424.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU633750 Genomic DNA. Translation: CAQ71270.1.
RefSeqYP_002007329.1. NC_010530.1.

3D structure databases

ProteinModelPortalB3R8B4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING164546.RALTA_B0651.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAQ71270; CAQ71270; RALTA_B0651.
GeneID6456844.
KEGGcti:RALTA_B0651.
PATRIC21535089. VBICupTai42494_4154.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMARRTHVWH.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycCTAI164546:GJNE-3867-MONOMER.
CTAI977880:GLC7-3867-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_CUPTR
AccessionPrimary (citable) accession number: B3R8B4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 2, 2008
Last modified: February 19, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families