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B3R8B4

- NAPA_CUPTR

UniProt

B3R8B4 - NAPA_CUPTR

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Protein
Periplasmic nitrate reductase
Gene
napA, RALTA_B0651
Organism
Cupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur (4Fe-4S) By similarity
Metal bindingi51 – 511Iron-sulfur (4Fe-4S) By similarity
Metal bindingi55 – 551Iron-sulfur (4Fe-4S) By similarity
Metal bindingi83 – 831Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciCTAI164546:GJNE-3867-MONOMER.
CTAI977880:GLC7-3867-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:RALTA_B0651
OrganismiCupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424))
Taxonomic identifieri164546 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000001692: Chromosome 2

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Tat-type signal Reviewed prediction
Add
BLAST
Chaini30 – 831802Periplasmic nitrate reductaseUniRule annotation
PRO_1000186352Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi164546.RALTA_B0651.

Structurei

3D structure databases

ProteinModelPortaliB3R8B4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 97574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiHPKTRVA.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B3R8B4-1 [UniParc]FASTAAdd to Basket

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MTLSRRDFIK QTAVAATASV AGVTLPAGAA NFVTDSEVTK LKWSKAPCRF    50
CGTGCGVTVA VRDNKVVATN GDPQAEVNKG LNCVKGYFLS KIMYGQDRLT 100
KPLLRMKNGQ YDKNGEFAPV TWERAFDEME RQFKRVLKEK GPTAVGMFGS 150
GQWTVWEGYA ASKLYKAGFR SNNIDPNARH CMASAVQGFM RTFGMDEPMG 200
CYDDFEAADA FVLWGSNMAE MHPILWTRIT DRRLSHPKTR VAVLSTFTHR 250
SFDLADIPVI FKPQTDLAMM NYIAHYIIKN NKVNKDFVNK HTVFKEGVTN 300
IGYGLRPDHP LQKAAKNAAD PGASRPITFD DFARFVAKYD ADTVSKLSGV 350
PKDKLDQLAE LYADTNIKVM SLWTMGFNQH TRGSWANNMV YNLHLLTGKI 400
ATPGNSPFSL TGQPSACGTA REVGTFSHRL PADMVVTNPK HREEAERIWK 450
LPPGTIPDKP GYHAVLQNRM LKDGKLNAYW VQVNNNMQAA ANLMEEGLPG 500
YRNPANFIVV SDAYPTVTAL AADLILPSAM WVEKEGAYGN AERRTQFWHQ 550
LVDAPGEARS DLWQLMEFSK RFKVEDVWPA DLIAKKPEYR GKTLFDVLYR 600
NGQVDKFPLK EVNAEYHNAE AKAFGFYVQK GLFEEYATFG RGHGHDLAPF 650
DAYHEARGLR WPVVNGKETR WRYREGSDPY VKAGTGYQFY GNPDGKAVIF 700
ALPYEPPAES PDKEYPYWLV TGRVLEHWHS GSMTRRVPEL YRAFPNAVVF 750
MHPEDAKAMG LRRGVEVEVV SRRGSMRSRL ETRGRDAPPR GLVFVPWFDA 800
SQLINKVTLD ATCPISLQTD FKKCAVKIVK V 831
Length:831
Mass (Da):93,381
Last modified:September 2, 2008 - v1
Checksum:i09339B5B92523DD3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU633750 Genomic DNA. Translation: CAQ71270.1.
RefSeqiYP_002007329.1. NC_010530.1.

Genome annotation databases

EnsemblBacteriaiCAQ71270; CAQ71270; RALTA_B0651.
GeneIDi6456844.
KEGGicti:RALTA_B0651.
PATRICi21535089. VBICupTai42494_4154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU633750 Genomic DNA. Translation: CAQ71270.1 .
RefSeqi YP_002007329.1. NC_010530.1.

3D structure databases

ProteinModelPortali B3R8B4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 164546.RALTA_B0651.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAQ71270 ; CAQ71270 ; RALTA_B0651 .
GeneIDi 6456844.
KEGGi cti:RALTA_B0651.
PATRICi 21535089. VBICupTai42494_4154.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi HPKTRVA.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci CTAI164546:GJNE-3867-MONOMER.
CTAI977880:GLC7-3867-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: R1 / LMG 19424.

Entry informationi

Entry nameiNAPA_CUPTR
AccessioniPrimary (citable) accession number: B3R8B4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 2, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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