B3R7J7 (GCST_CUPTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	RALTA_A3074

Organism

Cupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424)) [Complete proteome] [HAMAP]

Taxonomic identifier

164546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: EC transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Aminomethyltransferase HAMAP MF_00259		PRO_1000114091

Sequences

	Sequence		Length	Mass (Da)
	B3R7J7 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: E38DF3E58EC3EEBB	FASTA	375	39,958
	10 20 30 40 50 60 MTLQATPLNA IHRALGARMV DFGGWDMPVN YGSQIEEHHA VRNDAGMFDV SHMCVVDLAG 70 80 90 100 110 120 PNTRAFLRGL LANNVDKLQT PGKALYSCML DEHGGVIDDL IVYFFAEDRF RLVVNAGTAV 130 140 150 160 170 180 GDIDWIRARN EATGSGVTIT PRREDVAPAG VPPLAIVAVQ GPNARAKVWS TFPSTQPSDA 190 200 210 220 230 240 LKPFNAVVVQ DPALGEVMVA RTGYTGEDGF ELVVPAASVA GLWEKLQAAG VRPAGLGARD 250 260 270 280 290 300 TLRLEAGMNL YGQDMDIKVS PLDAGLAWTV DLQSERDFTG KAALAAAGSR QQFLGLILRD 310 320 330 340 350 360 KGGVLRAHQK VITPAGDGEI TSGTFSPSLS QSIAFARLPQ GVNVGDTVQV EIRDRKLNAT 370 VVKLPFVRHG KALVS

References

[1]

"Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia." Amadou C., Pascal G., Mangenot S., Glew M., Bontemps C., Capela D., Carrere S., Cruveiller S., Dossat C., Lajus A., Marchetti M., Poinsot V., Rouy Z., Servin B., Saad M., Schenowitz C., Barbe V., Batut J., Medigue C., Masson-Boivin C. Genome Res. 18:1472-1483(2008) [PubMed: 18490699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R1 / LMG 19424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU633749 Genomic DNA. Translation: CAQ70994.1.
RefSeq	YP_002007055.1. NC_010528.1.
3D structure databases
ProteinModelPortal	B3R7J7.
ModBase	Search...
Protein-protein interaction databases
STRING	B3R7J7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6454184.
GenomeReviews	Gene locus RALTA_A3074 in contig CU633749_GR.
KEGG	cti:RALTA_A3074.
PATRIC	21532838. VBICupTai42494_3040.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG299834.
OMA	KALYGGM.
ProtClustDB	PRK00389.
Family and domain databases
HAMAP	MF_00259. GcvT. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR022903. NH2_Me_Trfase. [Graphical view]
KO	K00605.
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. GcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_CUPTR

Accession

Primary (citable) accession number: B3R7J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program