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B3R6E8

- SYI_CUPTR

UniProt

B3R6E8 - SYI_CUPTR

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei599 – 5991Aminoacyl-adenylateUniRule annotation
Binding sitei643 – 6431ATPUniRule annotation
Metal bindingi929 – 9291ZincUniRule annotation
Metal bindingi932 – 9321ZincUniRule annotation
Metal bindingi949 – 9491ZincUniRule annotation
Metal bindingi952 – 9521ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCTAI164546:GJNE-2515-MONOMER.
CTAI977880:GLC7-2515-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:RALTA_A2521
OrganismiCupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424))
Taxonomic identifieri164546 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000001692: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 966966Isoleucine--tRNA ligasePRO_1000189144Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi164546.RALTA_A2521.

Structurei

3D structure databases

ProteinModelPortaliB3R6E8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi69 – 7911"HIGH" regionAdd
BLAST
Motifi640 – 6445"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKKRIELM.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3R6E8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDDKRAKSD KNEKNKYPVN LLDTPFPMRG DLPKREPQWV KQWQDKQLYK
60 70 80 90 100
KIRAARKGAK KFVLHDGPPY ANGDIHIGHA VNKVLKDMII KARGLTGLDA
110 120 130 140 150
VYVPGWDCHG MPIEIQIEKK FGKGLPVQEV QAKARAYATE QIKRQMVDFE
160 170 180 190 200
RLGVLGDWDH PYLTMNYRNE ADELRALGKI MEKGYVFRGL KPVNWCFDCG
210 220 230 240 250
SALAEAEVEY KDKVDLSIDV GFPFAEADKL AHAFKVPVEQ IDARPGWIVI
260 270 280 290 300
WTTTPWTIPS NQALNVHPEV EYALVDTPRG HLILATERVE EQLKVYALEG
310 320 330 340 350
KVIATATGAA LSEIRFHHPL AKMDGGYDRL SPIYLGDYVT TDTGSGIVHS
360 370 380 390 400
APAYGVEDFQ SCKAHGMPDS DIISPVMGNG VYAGTLPLFG GLSIWDANPR
410 420 430 440 450
IVEALQESGN LFNSHKYTHS YMHCWRHKTP IIYRATSQWF AGMDVDPAEE
460 470 480 490 500
NGKPVPTLRE TALAGIDATE FYPAWGKQRL HNMIANRPDW TLSRQRQWGV
510 520 530 540 550
PMAFFVHKET GALHPRTPEL LEAIAKRVEQ QGIEAWQTLD PAELLGDEAS
560 570 580 590 600
QYEKNRDTLD VWFDSGTTHW TVIRGSHRDD LYDPSADEAD GRLADLYLEG
610 620 630 640 650
SDQHRGWFHS SLLTASMLYG KPPYKALLTH GFTVDGEGRK MSKSIGNTVS
660 670 680 690 700
PQDIANKMGA EIIRLWVAST DYSGELSISD EILKRVVESY RRIRNTLRFL
710 720 730 740 750
LSNLSDYDHG KHALPAAEWL EIDRYAVALT AQLQKEVLSH YEAYEFHPVV
760 770 780 790 800
AKLQTFCSED LGGFYLDVLK DRLYTTAPDS KARRAAQNAL YHITQAMLHW
810 820 830 840 850
MAPFLSFTAE EAWQVFAHGT AHTDTIFTST YYAVPEVDDA DDLLQKWHTL
860 870 880 890 900
REVRAEVTRQ LEAVRVEGEI GSSLQAELTI QAGGPVLEAL QSLGDDLRFV
910 920 930 940 950
LLTSAAKVTA APEAGDLLVT VTPSAHAKCE RCWHYRADVG HNPDHPTLCG
960
RCDSNLFGAG EHRSHA
Length:966
Mass (Da):108,366
Last modified:September 2, 2008 - v1
Checksum:iF81651D467BAC7F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU633749 Genomic DNA. Translation: CAQ70452.1.
RefSeqiYP_002006513.1. NC_010528.1.

Genome annotation databases

EnsemblBacteriaiCAQ70452; CAQ70452; RALTA_A2521.
GeneIDi6454989.
KEGGicti:RALTA_A2521.
PATRICi21531684. VBICupTai42494_2481.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU633749 Genomic DNA. Translation: CAQ70452.1 .
RefSeqi YP_002006513.1. NC_010528.1.

3D structure databases

ProteinModelPortali B3R6E8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 164546.RALTA_A2521.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAQ70452 ; CAQ70452 ; RALTA_A2521 .
GeneIDi 6454989.
KEGGi cti:RALTA_A2521.
PATRICi 21531684. VBICupTai42494_2481.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KKRIELM.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CTAI164546:GJNE-2515-MONOMER.
CTAI977880:GLC7-2515-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: R1 / LMG 19424.

Entry informationi

Entry nameiSYI_CUPTR
AccessioniPrimary (citable) accession number: B3R6E8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 2, 2008
Last modified: November 26, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3