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B3R6E8

- SYI_CUPTR

UniProt

B3R6E8 - SYI_CUPTR

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (02 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei599 – 5991Aminoacyl-adenylateUniRule annotation
    Binding sitei643 – 6431ATPUniRule annotation
    Metal bindingi929 – 9291ZincUniRule annotation
    Metal bindingi932 – 9321ZincUniRule annotation
    Metal bindingi949 – 9491ZincUniRule annotation
    Metal bindingi952 – 9521ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCTAI164546:GJNE-2515-MONOMER.
    CTAI977880:GLC7-2515-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:RALTA_A2521
    OrganismiCupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424))
    Taxonomic identifieri164546 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
    ProteomesiUP000001692: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 966966Isoleucine--tRNA ligasePRO_1000189144Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi164546.RALTA_A2521.

    Structurei

    3D structure databases

    ProteinModelPortaliB3R6E8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi69 – 7911"HIGH" regionAdd
    BLAST
    Motifi640 – 6445"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKKRIELM.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B3R6E8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDDKRAKSD KNEKNKYPVN LLDTPFPMRG DLPKREPQWV KQWQDKQLYK    50
    KIRAARKGAK KFVLHDGPPY ANGDIHIGHA VNKVLKDMII KARGLTGLDA 100
    VYVPGWDCHG MPIEIQIEKK FGKGLPVQEV QAKARAYATE QIKRQMVDFE 150
    RLGVLGDWDH PYLTMNYRNE ADELRALGKI MEKGYVFRGL KPVNWCFDCG 200
    SALAEAEVEY KDKVDLSIDV GFPFAEADKL AHAFKVPVEQ IDARPGWIVI 250
    WTTTPWTIPS NQALNVHPEV EYALVDTPRG HLILATERVE EQLKVYALEG 300
    KVIATATGAA LSEIRFHHPL AKMDGGYDRL SPIYLGDYVT TDTGSGIVHS 350
    APAYGVEDFQ SCKAHGMPDS DIISPVMGNG VYAGTLPLFG GLSIWDANPR 400
    IVEALQESGN LFNSHKYTHS YMHCWRHKTP IIYRATSQWF AGMDVDPAEE 450
    NGKPVPTLRE TALAGIDATE FYPAWGKQRL HNMIANRPDW TLSRQRQWGV 500
    PMAFFVHKET GALHPRTPEL LEAIAKRVEQ QGIEAWQTLD PAELLGDEAS 550
    QYEKNRDTLD VWFDSGTTHW TVIRGSHRDD LYDPSADEAD GRLADLYLEG 600
    SDQHRGWFHS SLLTASMLYG KPPYKALLTH GFTVDGEGRK MSKSIGNTVS 650
    PQDIANKMGA EIIRLWVAST DYSGELSISD EILKRVVESY RRIRNTLRFL 700
    LSNLSDYDHG KHALPAAEWL EIDRYAVALT AQLQKEVLSH YEAYEFHPVV 750
    AKLQTFCSED LGGFYLDVLK DRLYTTAPDS KARRAAQNAL YHITQAMLHW 800
    MAPFLSFTAE EAWQVFAHGT AHTDTIFTST YYAVPEVDDA DDLLQKWHTL 850
    REVRAEVTRQ LEAVRVEGEI GSSLQAELTI QAGGPVLEAL QSLGDDLRFV 900
    LLTSAAKVTA APEAGDLLVT VTPSAHAKCE RCWHYRADVG HNPDHPTLCG 950
    RCDSNLFGAG EHRSHA 966
    Length:966
    Mass (Da):108,366
    Last modified:September 2, 2008 - v1
    Checksum:iF81651D467BAC7F6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU633749 Genomic DNA. Translation: CAQ70452.1.
    RefSeqiYP_002006513.1. NC_010528.1.

    Genome annotation databases

    EnsemblBacteriaiCAQ70452; CAQ70452; RALTA_A2521.
    GeneIDi6454989.
    KEGGicti:RALTA_A2521.
    PATRICi21531684. VBICupTai42494_2481.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU633749 Genomic DNA. Translation: CAQ70452.1 .
    RefSeqi YP_002006513.1. NC_010528.1.

    3D structure databases

    ProteinModelPortali B3R6E8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 164546.RALTA_A2521.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAQ70452 ; CAQ70452 ; RALTA_A2521 .
    GeneIDi 6454989.
    KEGGi cti:RALTA_A2521.
    PATRICi 21531684. VBICupTai42494_2481.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KKRIELM.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CTAI164546:GJNE-2515-MONOMER.
    CTAI977880:GLC7-2515-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: R1 / LMG 19424.

    Entry informationi

    Entry nameiSYI_CUPTR
    AccessioniPrimary (citable) accession number: B3R6E8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: September 2, 2008
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3