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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CIP 107171 / LMG 19424 / R1) (Ralstonia taiwanensis (strain LMG 19424))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei599Aminoacyl-adenylateUniRule annotation1
Binding sitei643ATPUniRule annotation1
Metal bindingi929ZincUniRule annotation1
Metal bindingi932ZincUniRule annotation1
Metal bindingi949ZincUniRule annotation1
Metal bindingi952ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCTAI977880:RALTA_RS12255-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:RALTA_A2521
OrganismiCupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CIP 107171 / LMG 19424 / R1) (Ralstonia taiwanensis (strain LMG 19424))
Taxonomic identifieri977880 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000001692 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001891441 – 966Isoleucine--tRNA ligaseAdd BLAST966

Proteomic databases

PRIDEiB3R6E8

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi977880.RALTA_A2521

Structurei

3D structure databases

ProteinModelPortaliB3R6E8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi69 – 79"HIGH" regionAdd BLAST11
Motifi640 – 644"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B3R6E8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDDKRAKSD KNEKNKYPVN LLDTPFPMRG DLPKREPQWV KQWQDKQLYK
60 70 80 90 100
KIRAARKGAK KFVLHDGPPY ANGDIHIGHA VNKVLKDMII KARGLTGLDA
110 120 130 140 150
VYVPGWDCHG MPIEIQIEKK FGKGLPVQEV QAKARAYATE QIKRQMVDFE
160 170 180 190 200
RLGVLGDWDH PYLTMNYRNE ADELRALGKI MEKGYVFRGL KPVNWCFDCG
210 220 230 240 250
SALAEAEVEY KDKVDLSIDV GFPFAEADKL AHAFKVPVEQ IDARPGWIVI
260 270 280 290 300
WTTTPWTIPS NQALNVHPEV EYALVDTPRG HLILATERVE EQLKVYALEG
310 320 330 340 350
KVIATATGAA LSEIRFHHPL AKMDGGYDRL SPIYLGDYVT TDTGSGIVHS
360 370 380 390 400
APAYGVEDFQ SCKAHGMPDS DIISPVMGNG VYAGTLPLFG GLSIWDANPR
410 420 430 440 450
IVEALQESGN LFNSHKYTHS YMHCWRHKTP IIYRATSQWF AGMDVDPAEE
460 470 480 490 500
NGKPVPTLRE TALAGIDATE FYPAWGKQRL HNMIANRPDW TLSRQRQWGV
510 520 530 540 550
PMAFFVHKET GALHPRTPEL LEAIAKRVEQ QGIEAWQTLD PAELLGDEAS
560 570 580 590 600
QYEKNRDTLD VWFDSGTTHW TVIRGSHRDD LYDPSADEAD GRLADLYLEG
610 620 630 640 650
SDQHRGWFHS SLLTASMLYG KPPYKALLTH GFTVDGEGRK MSKSIGNTVS
660 670 680 690 700
PQDIANKMGA EIIRLWVAST DYSGELSISD EILKRVVESY RRIRNTLRFL
710 720 730 740 750
LSNLSDYDHG KHALPAAEWL EIDRYAVALT AQLQKEVLSH YEAYEFHPVV
760 770 780 790 800
AKLQTFCSED LGGFYLDVLK DRLYTTAPDS KARRAAQNAL YHITQAMLHW
810 820 830 840 850
MAPFLSFTAE EAWQVFAHGT AHTDTIFTST YYAVPEVDDA DDLLQKWHTL
860 870 880 890 900
REVRAEVTRQ LEAVRVEGEI GSSLQAELTI QAGGPVLEAL QSLGDDLRFV
910 920 930 940 950
LLTSAAKVTA APEAGDLLVT VTPSAHAKCE RCWHYRADVG HNPDHPTLCG
960
RCDSNLFGAG EHRSHA
Length:966
Mass (Da):108,366
Last modified:September 2, 2008 - v1
Checksum:iF81651D467BAC7F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU633749 Genomic DNA Translation: CAQ70452.1
RefSeqiWP_012353750.1, NC_010528.1

Genome annotation databases

EnsemblBacteriaiCAQ70452; CAQ70452; RALTA_A2521
GeneIDi29761966
KEGGicti:RALTA_A2521

Similar proteinsi

Entry informationi

Entry nameiSYI_CUPTR
AccessioniPrimary (citable) accession number: B3R6E8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 2, 2008
Last modified: June 20, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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