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B3R6E8

- SYI_CUPTR

UniProt

B3R6E8 - SYI_CUPTR

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Protein
Isoleucine--tRNA ligase
Gene
ileS, RALTA_A2521
Organism
Cupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei599 – 5991Aminoacyl-adenylate By similarity
Binding sitei643 – 6431ATP By similarity
Metal bindingi929 – 9291Zinc By similarity
Metal bindingi932 – 9321Zinc By similarity
Metal bindingi949 – 9491Zinc By similarity
Metal bindingi952 – 9521Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCTAI164546:GJNE-2515-MONOMER.
CTAI977880:GLC7-2515-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:RALTA_A2521
OrganismiCupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424))
Taxonomic identifieri164546 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000001692: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 966966Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189144Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi164546.RALTA_A2521.

Structurei

3D structure databases

ProteinModelPortaliB3R6E8.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi69 – 7911"HIGH" regionUniRule annotation
Add
BLAST
Motifi640 – 6445"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKKRIELM.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3R6E8-1 [UniParc]FASTAAdd to Basket

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MSDDKRAKSD KNEKNKYPVN LLDTPFPMRG DLPKREPQWV KQWQDKQLYK    50
KIRAARKGAK KFVLHDGPPY ANGDIHIGHA VNKVLKDMII KARGLTGLDA 100
VYVPGWDCHG MPIEIQIEKK FGKGLPVQEV QAKARAYATE QIKRQMVDFE 150
RLGVLGDWDH PYLTMNYRNE ADELRALGKI MEKGYVFRGL KPVNWCFDCG 200
SALAEAEVEY KDKVDLSIDV GFPFAEADKL AHAFKVPVEQ IDARPGWIVI 250
WTTTPWTIPS NQALNVHPEV EYALVDTPRG HLILATERVE EQLKVYALEG 300
KVIATATGAA LSEIRFHHPL AKMDGGYDRL SPIYLGDYVT TDTGSGIVHS 350
APAYGVEDFQ SCKAHGMPDS DIISPVMGNG VYAGTLPLFG GLSIWDANPR 400
IVEALQESGN LFNSHKYTHS YMHCWRHKTP IIYRATSQWF AGMDVDPAEE 450
NGKPVPTLRE TALAGIDATE FYPAWGKQRL HNMIANRPDW TLSRQRQWGV 500
PMAFFVHKET GALHPRTPEL LEAIAKRVEQ QGIEAWQTLD PAELLGDEAS 550
QYEKNRDTLD VWFDSGTTHW TVIRGSHRDD LYDPSADEAD GRLADLYLEG 600
SDQHRGWFHS SLLTASMLYG KPPYKALLTH GFTVDGEGRK MSKSIGNTVS 650
PQDIANKMGA EIIRLWVAST DYSGELSISD EILKRVVESY RRIRNTLRFL 700
LSNLSDYDHG KHALPAAEWL EIDRYAVALT AQLQKEVLSH YEAYEFHPVV 750
AKLQTFCSED LGGFYLDVLK DRLYTTAPDS KARRAAQNAL YHITQAMLHW 800
MAPFLSFTAE EAWQVFAHGT AHTDTIFTST YYAVPEVDDA DDLLQKWHTL 850
REVRAEVTRQ LEAVRVEGEI GSSLQAELTI QAGGPVLEAL QSLGDDLRFV 900
LLTSAAKVTA APEAGDLLVT VTPSAHAKCE RCWHYRADVG HNPDHPTLCG 950
RCDSNLFGAG EHRSHA 966
Length:966
Mass (Da):108,366
Last modified:September 2, 2008 - v1
Checksum:iF81651D467BAC7F6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU633749 Genomic DNA. Translation: CAQ70452.1.
RefSeqiYP_002006513.1. NC_010528.1.

Genome annotation databases

EnsemblBacteriaiCAQ70452; CAQ70452; RALTA_A2521.
GeneIDi6454989.
KEGGicti:RALTA_A2521.
PATRICi21531684. VBICupTai42494_2481.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU633749 Genomic DNA. Translation: CAQ70452.1 .
RefSeqi YP_002006513.1. NC_010528.1.

3D structure databases

ProteinModelPortali B3R6E8.
ModBasei Search...

Protein-protein interaction databases

STRINGi 164546.RALTA_A2521.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAQ70452 ; CAQ70452 ; RALTA_A2521 .
GeneIDi 6454989.
KEGGi cti:RALTA_A2521.
PATRICi 21531684. VBICupTai42494_2481.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KKRIELM.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CTAI164546:GJNE-2515-MONOMER.
CTAI977880:GLC7-2515-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: R1 / LMG 19424.

Entry informationi

Entry nameiSYI_CUPTR
AccessioniPrimary (citable) accession number: B3R6E8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 2, 2008
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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