B3R5N3 (SYQ_CUPTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	RALTA_A2274

Organism

Cupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424)) [Complete proteome] [HAMAP]

Taxonomic identifier

164546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity. HAMAP MF_00126
Subcellular location	Cytoplasm By similarity HAMAP MF_00126.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	Glutamine--tRNA ligase HAMAP MF_00126		PRO_1000095487
Regions
	Motif	51 – 61	11	"HIGH" region HAMAP MF_00126
	Motif	299 – 303	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	302	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3R5N3 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: C651D57FB0F16A44	FASTA	585	66,312
	10 20 30 40 50 60 MSHDNKPTDS NPAASNFLRS IIDQDLAAGT YAGRQDKQGE PLPTVITRFP PEPNGYLHIG 70 80 90 100 110 120 HAKSICLNFG LARDYGGRCH LRFDDTNPVK EDTEYVESII DAVHWLGFSW DSEGKDGQKQ 130 140 150 160 170 180 PHLYYASDYF DQLYAFAETL IERGAAYVDS QSAEQIAASR GNFSEPGKPS PFRDRSVEEN 190 200 210 220 230 240 LQLFRDMRAG KYADGEHVLR AKIDMAAPNI VMRDPVLYRI RHAHHHRTGD KWCIYPMYDF 250 260 270 280 290 300 THCISDALEN ITHSLCTLEF ENNRPLYDWV LEHLRDSGVF RDPLPHQYEF ARLNLTYAIT 310 320 330 340 350 360 SKRKLKQLVD EQRVDGWDDP RMPTLVGVRR RGYTPESIQL FCDRVGVAKA DSWIDMSTLE 370 380 390 400 410 420 GSVRDDLDGR AARGVAVLDP LKLIIDNYPE GQSEECSAPV HPKKPELGKR VFPLSRELWI 430 440 450 460 470 480 EREDFNETPP KGYFRLFPGN KVRLKYGYVI ECTGVDKDAD GNVIAVHASY LPDTKSGTPG 490 500 510 520 530 540 ADSVKVKGVI HWVSAAHAYE AEVRLYDRLF NDPNPDAGGK NFLDALNPDS KQVITAYLEP 550 560 570 580 GLREAQPEDR FQFERHGYFV ADRSDSTPGK PVFNRIVGLK DSWGK

References

[1]

"Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia." Amadou C., Pascal G., Mangenot S., Glew M., Bontemps C., Capela D., Carrere S., Cruveiller S., Dossat C., Lajus A., Marchetti M., Poinsot V., Rouy Z., Servin B., Saad M., Schenowitz C., Barbe V., Batut J., Medigue C., Masson-Boivin C. Genome Res. 18:1472-1483(2008) [PubMed: 18490699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R1 / LMG 19424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU633749 Genomic DNA. Translation: CAQ70208.1.
RefSeq	YP_002006271.1. NC_010528.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3R5N3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6453642.
GenomeReviews	Gene locus RALTA_A2274 in contig CU633749_GR.
KEGG	cti:RALTA_A2274.
PATRIC	21531186. VBICupTai42494_2235.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG334108.
OMA	RMPTIAG.
ProtClustDB	PRK05347.
Family and domain databases
HAMAP	MF_00126. Gln_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_synth_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 2 hits. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01886.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. GlnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_CUPTR

Accession

Primary (citable) accession number: B3R5N3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program