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B3R5N3 (SYQ_CUPTR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--tRNA ligase

EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Gene names
Name:glnS
Ordered Locus Names:RALTA_A2274
OrganismCupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424)) [Complete proteome] [HAMAP]
Taxonomic identifier164546 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length585 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00126

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00126.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutaminyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 585585Glutamine--tRNA ligase HAMAP-Rule MF_00126
PRO_1000095487

Regions

Motif51 – 6111"HIGH" region HAMAP-Rule MF_00126
Motif299 – 3035"KMSKS" region HAMAP-Rule MF_00126

Sites

Binding site3021ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B3R5N3 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: C651D57FB0F16A44

FASTA58566,312
        10         20         30         40         50         60 
MSHDNKPTDS NPAASNFLRS IIDQDLAAGT YAGRQDKQGE PLPTVITRFP PEPNGYLHIG 

        70         80         90        100        110        120 
HAKSICLNFG LARDYGGRCH LRFDDTNPVK EDTEYVESII DAVHWLGFSW DSEGKDGQKQ 

       130        140        150        160        170        180 
PHLYYASDYF DQLYAFAETL IERGAAYVDS QSAEQIAASR GNFSEPGKPS PFRDRSVEEN 

       190        200        210        220        230        240 
LQLFRDMRAG KYADGEHVLR AKIDMAAPNI VMRDPVLYRI RHAHHHRTGD KWCIYPMYDF 

       250        260        270        280        290        300 
THCISDALEN ITHSLCTLEF ENNRPLYDWV LEHLRDSGVF RDPLPHQYEF ARLNLTYAIT 

       310        320        330        340        350        360 
SKRKLKQLVD EQRVDGWDDP RMPTLVGVRR RGYTPESIQL FCDRVGVAKA DSWIDMSTLE 

       370        380        390        400        410        420 
GSVRDDLDGR AARGVAVLDP LKLIIDNYPE GQSEECSAPV HPKKPELGKR VFPLSRELWI 

       430        440        450        460        470        480 
EREDFNETPP KGYFRLFPGN KVRLKYGYVI ECTGVDKDAD GNVIAVHASY LPDTKSGTPG 

       490        500        510        520        530        540 
ADSVKVKGVI HWVSAAHAYE AEVRLYDRLF NDPNPDAGGK NFLDALNPDS KQVITAYLEP 

       550        560        570        580 
GLREAQPEDR FQFERHGYFV ADRSDSTPGK PVFNRIVGLK DSWGK 

« Hide

References

[1]"Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia."
Amadou C., Pascal G., Mangenot S., Glew M., Bontemps C., Capela D., Carrere S., Cruveiller S., Dossat C., Lajus A., Marchetti M., Poinsot V., Rouy Z., Servin B., Saad M., Schenowitz C., Barbe V., Batut J., Medigue C., Masson-Boivin C.
Genome Res. 18:1472-1483(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: R1 / LMG 19424.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU633749 Genomic DNA. Translation: CAQ70208.1.
RefSeqYP_002006271.1. NC_010528.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING164546.RALTA_A2274.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAQ70208; CAQ70208; RALTA_A2274.
GeneID6453642.
KEGGcti:RALTA_A2274.
PATRIC21531186. VBICupTai42494_2235.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000259232.
KOK01886.
OMAVTHSICT.
OrthoDBEOG6DRPF7.
ProtClustDBPRK05347.

Enzyme and pathway databases

BioCycCTAI164546:GJNE-2266-MONOMER.
CTAI977880:GLC7-2266-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPMF_00126. Gln_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYQ_CUPTR
AccessionPrimary (citable) accession number: B3R5N3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 2, 2008
Last modified: February 19, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries