B3R368 (AROA_CUPTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	RALTA_A0776

Organism

Cupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424)) [Complete proteome] [HAMAP]

Taxonomic identifier

164546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_1000099696

Sequences

	Sequence		Length	Mass (Da)
	B3R368 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: 4342DA98AC1725D6	FASTA	434	46,425
	10 20 30 40 50 60 MEHLTLGPLT RATGTVRLPG SKSISNRVLL LAALANGETR VRDLLDSDDT RVMLQALRTL 70 80 90 100 110 120 GVAWRQDGPD YIVTGAGGNF PVKSAELFMG NAGTAIRPLT AALALQGGSY KLSGVPRMHE 130 140 150 160 170 180 RPIGDLVDGL RQVGAVIDYL GNEGFPPLHI QPASLRIDAP IRVRGDVSSQ FLTALLMSLP 190 200 210 220 230 240 LAQSASGRIE IEVVGELISK PYIEITLNLL ARFGIEVERQ GWERFILPAG TAYRSPGEIF 250 260 270 280 290 300 VEGDASSASY FLAAGAIGGG PVRVEGVGMA SIQGDVRFAD ALNRMGANVM AGDNWIEVRG 310 320 330 340 350 360 TERDDGRLHG IELDCNHIPD AAMTLAVAAL FAEGTTTLTN IASWRVKETD RIAAMATELR 370 380 390 400 410 420 KLGAVVEEGA DYLRVTPPQP WQTPADGIGT YDDHRMAMCF SLAAFGPLPV RINDPGCVAK 430 TFPDYFSVFA GVTR

References

[1]

"Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia." Amadou C., Pascal G., Mangenot S., Glew M., Bontemps C., Capela D., Carrere S., Cruveiller S., Dossat C., Lajus A., Marchetti M., Poinsot V., Rouy Z., Servin B., Saad M., Schenowitz C., Barbe V., Batut J., Medigue C., Masson-Boivin C. Genome Res. 18:1472-1483(2008) [PubMed: 18490699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R1 / LMG 19424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU633749 Genomic DNA. Translation: CAQ68748.1.
RefSeq	YP_002004817.1. NC_010528.1.
3D structure databases
ProteinModelPortal	B3R368.
ModBase	Search...
Protein-protein interaction databases
STRING	B3R368.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6455185.
GenomeReviews	Gene locus RALTA_A0776 in contig CU633749_GR.
KEGG	cti:RALTA_A0776.
PATRIC	21528178. VBICupTai42494_0771.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646626.
OMA	GADIEWG.
ProtClustDB	PRK02427.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_CUPTR

Accession

Primary (citable) accession number: B3R368

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program