B3R2A7 (LPXD_CUPTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	RALTA_A1681

Organism

Cupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424)) [Complete proteome] [HAMAP]

Taxonomic identifier

164546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_1000127673
Sites
	Active site	252	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3R2A7 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: DBBEBEF9432C8CE0	FASTA	363	37,702
	10 20 30 40 50 60 MQTPTLGQLA TENGAQVVGD PDLAITGLAP LDQAGPGELS FLSNPLYLQQ ALDAKAGAVI 70 80 90 100 110 120 VSAADLERVR AEGKADGRNW LVARNPYVCF ARVAQRFDRA ANTDARTGID ARATVAPDAV 130 140 150 160 170 180 VPASCYIGPN VVIEAGARLG ERVRILANGY VGAHAQIGDD ALLYANVSVY HHCVVGARAI 190 200 210 220 230 240 LHSGVVIGAD GFGFAPDISA SGVEYVKIPQ TGRAVLGDDV EVGANTAIDR GAMADTVIED 250 260 270 280 290 300 GCKIDNQVQI AHNVRVGAHT VIAGCAAVSG STRIGRFCVI GGAANFAGHL TIADRTTVSG 310 320 330 340 350 360 GTSITKSITK PGGHFTSVFP FLPHGEWERN AAIVRGLSKL RERVVALERR LRGQAAGSQP SQD

References

[1]

"Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia." Amadou C., Pascal G., Mangenot S., Glew M., Bontemps C., Capela D., Carrere S., Cruveiller S., Dossat C., Lajus A., Marchetti M., Poinsot V., Rouy Z., Servin B., Saad M., Schenowitz C., Barbe V., Batut J., Medigue C., Masson-Boivin C. Genome Res. 18:1472-1483(2008) [PubMed: 18490699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R1 / LMG 19424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU633749 Genomic DNA. Translation: CAQ69624.1.
RefSeq	YP_002005689.1. NC_010528.1.
3D structure databases
ProteinModelPortal	B3R2A7.
ModBase	Search...
Protein-protein interaction databases
STRING	B3R2A7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6454444.
GenomeReviews	Gene locus RALTA_A1681 in contig CU633749_GR.
KEGG	cti:RALTA_A1681.
PATRIC	21529964. VBICupTai42494_1644.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG469615.
OMA	SYPAKIM.
ProtClustDB	PRK00892.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 4 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_CUPTR

Accession

Primary (citable) accession number: B3R2A7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program