B3R1Y0 (GLO2_CUPTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	RALTA_A1995

Organism

Cupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424)) [Complete proteome] [HAMAP]

Taxonomic identifier

164546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_1000144754
Sites
	Metal binding	53	1	Zinc 1 By similarity
	Metal binding	55	1	Zinc 1 By similarity
	Metal binding	57	1	Zinc 2 By similarity
	Metal binding	58	1	Zinc 2 By similarity
	Metal binding	118	1	Zinc 1 By similarity
	Metal binding	140	1	Zinc 1 By similarity
	Metal binding	140	1	Zinc 2 By similarity
	Metal binding	178	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3R1Y0-1 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: FAA8D70D18B2A0C8	FASTA	266	27,851
	10 20 30 40 50 60 MLKVEPIPAF QDNYIWAIHD GHCAAVVDPG EAAPVERFLA QSGLALGAIV ITHHHGDHQG 70 80 90 100 110 120 GVAGLLAAHP RAPSGGPLPV LGPAGERIGG RTQALREGDV ATLEAPALTL RVLDVPGHTA 130 140 150 160 170 180 GHIAYVADLG EAGPALFCGD TLFASGCGRL FEGTPAQMLA SLDKLAALPG DTRVYCAHEY 190 200 210 220 230 240 TRSNVRFARA VEPGNAALAA WEARVEALRA TNQPTLPTTI AHERQVNPFL RSREPAVRAA 250 260 VSAHGGTAAD DAQNFGALRG WKDNFR

References

[1]

"Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia." Amadou C., Pascal G., Mangenot S., Glew M., Bontemps C., Capela D., Carrere S., Cruveiller S., Dossat C., Lajus A., Marchetti M., Poinsot V., Rouy Z., Servin B., Saad M., Schenowitz C., Barbe V., Batut J., Medigue C., Masson-Boivin C. Genome Res. 18:1472-1483(2008) [PubMed: 18490699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU633749 Genomic DNA. Translation: CAQ69936.1.
RefSeq	YP_002005999.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6454726.
GenomeReviews	Gene locus RALTA_A1995 in contig CU633749_GR.
KEGG	cti:RALTA_A1995.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG753931.
OMA	FGHEYTL.
ProtClustDB	CLSK896990.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Blactmase-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_CUPTR

Accession

Primary (citable) accession number: B3R1Y0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	August 10, 2010
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)