B3R1X2 (ACSA_CUPTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-coenzyme A synthetase
EC=6.2.1.1

Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme

Gene names

Name:	acsA
Ordered Locus Names:	RALTA_A2028

Organism

Cupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424)) [Complete proteome] [HAMAP]

Taxonomic identifier

164546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 660	660	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_1000137262
Sites
	Active site	530	1	By similarity
Amino acid modifications
	Modified residue	625	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3R1X2 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: CDBE1FB125782102	FASTA	660	72,452
	10 20 30 40 50 60 MSAIESVMQE HRVFNPPEAF ASQAAIPSMD AYRALCDEAE RDYEGFWARY ARELLHWNKP 70 80 90 100 110 120 FTKVLDESNA PFYKWFEDGE LNASYNCLDR NLQNGNADKV AIVFEADDGT VTRVTYRELH 130 140 150 160 170 180 AKVCRLANGL KTLGIRKGDR VVIYMPMSVE GVAAMQACAR LGATHSVVFG GFSAKSLQER 190 200 210 220 230 240 LVDVGAVALI TADEQMRGGK ALPLKAIADD ALALGGCEAV KNVIVYRRTG GNVGWTEGRD 250 260 270 280 290 300 RWLDDVCANQ PDTCEAEPVG AEHPLFVLYT SGSTGKPKGV QHSTGGYLLW ALMTMKWTFD 310 320 330 340 350 360 IKPDDLFWCT ADIGWVTGHT YIAYGPLAAG ATQVVFEGVP TYPNAGRFWD MIARHKVSIF 370 380 390 400 410 420 YTAPTAIRSL IKAAEADEKI HPKQYDLSSL RLLGTVGEPI NPEAWMWYYK NVGNENCPIV 430 440 450 460 470 480 DTFWQTETGG HMITPLPGAT PLVPGSCTLP LPGIMAAIVD ETGHDVPNGS GGILVVKRPW 490 500 510 520 530 540 PAMIRTIWGD PERFKKSYFP EELGGKLYLA GDGSIRDKDT GYFTIMGRID DVLNVSGHRM 550 560 570 580 590 600 GTMEIESALV ANPLVAEAAV VGRPDDMTGE AICAFVVLKR SRPSGEEAAK LATELRNWVG 610 620 630 640 650 660 KEIGPIAKPK DIRFGDNLPK TRSGKIMRRL LRSLAKGEEI TQDTSTLENP AILEQLKQAQ

References

[1]

"Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia." Amadou C., Pascal G., Mangenot S., Glew M., Bontemps C., Capela D., Carrere S., Cruveiller S., Dossat C., Lajus A., Marchetti M., Poinsot V., Rouy Z., Servin B., Saad M., Schenowitz C., Barbe V., Batut J., Medigue C., Masson-Boivin C. Genome Res. 18:1472-1483(2008) [PubMed: 18490699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R1 / LMG 19424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU633749 Genomic DNA. Translation: CAQ69969.1.
RefSeq	YP_002006032.1. NC_010528.1.
3D structure databases
ProteinModelPortal	B3R1X2.
ModBase	Search...
Protein-protein interaction databases
STRING	B3R1X2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6453493.
GenomeReviews	Gene locus RALTA_A2028 in contig CU633749_GR.
KEGG	cti:RALTA_A2028.
PATRIC	21530682. VBICupTai42494_1989.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG547964.
OMA	VISVSGH.
ProtClustDB	PRK00174.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig. IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01895.
PANTHER	PTHR24095:SF42. PTHR24095:SF42. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_CUPTR

Accession

Primary (citable) accession number: B3R1X2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program