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B3QY14

- PNP_CHLT3

UniProt

B3QY14 - PNP_CHLT3

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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Chloroherpeton thalassium (strain ATCC 35110 / GB-78)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi503 – 5031MagnesiumUniRule annotation
Metal bindingi509 – 5091MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciCTHA517418:GHTO-1093-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Ctha_1078
OrganismiChloroherpeton thalassium (strain ATCC 35110 / GB-78)
Taxonomic identifieri517418 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChloroherpeton
ProteomesiUP000001208: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 729729Polyribonucleotide nucleotidyltransferasePRO_1000147903Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi517418.Ctha_1078.

Structurei

3D structure databases

ProteinModelPortaliB3QY14.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini570 – 63364KHUniRule annotationAdd
BLAST
Domaini639 – 71476S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiGTENTIC.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3QY14-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVFTKEIDLG QGKVLKIETG KMAKQADGSA VVSLEETMVL ATVVSKKDAP
60 70 80 90 100
PANQNFFPLQ VEYREKYSAA GKFPGGFIKR EGRPSDKEIL SARLIDRALR
110 120 130 140 150
PLFPDGYYQE TQIIVTVISS DQLNDADVLG GIAASCALMA SDIPFENPMS
160 170 180 190 200
EVRVGRINGD FLIFPHVQEL EESDMDICIG GTADTICMLE GEMSEISEAE
210 220 230 240 250
MIGAIKFGHD VIRKICEAQR ELREVVGKPK RVFEPTIIPD DMKAAIREVA
260 270 280 290 300
EARLKELAYQ PLKKEERADK TAEVYADASA AVLRKYKAEI TDEILAENPE
310 320 330 340 350
KAIYLNEKAI NDYIHDIEKH VMREMILADA KRLDGRRLDE IRPISIELGL
360 370 380 390 400
IPRAHGSALF TRGETQALVT LTLGTKKDAQ LTDTLFDDAD KRFMLHYNFP
410 420 430 440 450
PFSVGEVGRM GSVSRREIGH GNLAERAIKK VVPAENAFPY TMRVVSDILE
460 470 480 490 500
SNGSSSMASV CGGTLAAMDG GVPIKSPVAG IAMGLIKEGE RYAVLSDILG
510 520 530 540 550
NEDHLGDMDF KVAGTIDGIT ACQMDIKIDG LDYAIVEKAL EQARQGRMHI
560 570 580 590 600
LKIMDEAITS PRQELAKYAP RLTTIQIPVD AIGAVIGKGG ETIRALTSET
610 620 630 640 650
NTEIDIADDG TVTIASVSSE GSAAAVDAIK LLVSDPEVGM VYNGKVKEVR
660 670 680 690 700
DDLGAIVEIL PGVTGLLHIS ELSHQRGTKP SDILKVGQKT KVKLIRMAKD
710 720
PKDPRRTRYS LSMKVLVEPP AEDGQKENE
Length:729
Mass (Da):79,620
Last modified:September 2, 2008 - v1
Checksum:i626C80CCC19532CB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001100 Genomic DNA. Translation: ACF13542.1.
RefSeqiWP_012499626.1. NC_011026.1.
YP_001995989.1. NC_011026.1.

Genome annotation databases

EnsemblBacteriaiACF13542; ACF13542; Ctha_1078.
GeneIDi6422134.
KEGGicts:Ctha_1078.
PATRICi21430911. VBIChlTha99257_1272.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001100 Genomic DNA. Translation: ACF13542.1 .
RefSeqi WP_012499626.1. NC_011026.1.
YP_001995989.1. NC_011026.1.

3D structure databases

ProteinModelPortali B3QY14.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 517418.Ctha_1078.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACF13542 ; ACF13542 ; Ctha_1078 .
GeneIDi 6422134.
KEGGi cts:Ctha_1078.
PATRICi 21430911. VBIChlTha99257_1272.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218327.
KOi K00962.
OMAi GTENTIC.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci CTHA517418:GHTO-1093-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Chloroherpeton thalassium ATCC 35110."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Liu Z., Li T., Zhao F.
    , Overmann J., Bryant D.A., Richardson P.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35110 / GB-78.

Entry informationi

Entry nameiPNP_CHLT3
AccessioniPrimary (citable) accession number: B3QY14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 2, 2008
Last modified: October 29, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3