B3QWT5 (MURD_CHLT3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	Ctha_0831

Organism

Chloroherpeton thalassium (strain ATCC 35110 / GB-78) [Complete proteome] [HAMAP]

Taxonomic identifier

517418 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chloroherpeton

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_1000130841
Regions
	Nucleotide binding	117 – 123	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B3QWT5 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: 8E452DF6D421009F	FASTA	468	51,901
	10 20 30 40 50 60 MTHEEIKGKR CAIIGGKRSG IAAAKLLSRA GAYVFLSEKS VPENQATLES DLRAHGIECE 70 80 90 100 110 120 FGQHSEEVFD ADFAIISPGV PSSAPVIKQL ERAQVPVYSE IELASWFCKA KIIAITGTDG 130 140 150 160 170 180 KTTVTTLTKK IFEADGNENG YHAYALGNIG QPFSDTVESL SENDVAVIEI SSFQLEHCTS 190 200 210 220 230 240 FRPNVTVITN ITPDHLDRYD GNIQKYAAAK YRIYQNQTAS DWLIYNDDNE ILHQHFTDPE 250 260 270 280 290 300 RRASLPMKLV ALSLEKNLGD EYEHCAYKEN GRLVLKLTNE KEWLIEENQI STKQFRGQHN 310 320 330 340 350 360 IYNALTAAAA ASALGIDKKF IEKSILSFEG VEHRLEFVRA VNQVDYINDS KATTVNALWY 370 380 390 400 410 420 ALDTVTPKIV LIAGGRDKGN DYTKVFSFVK EKVRAVVAIG ESQEKVVQAF GNLTKVMKAF 430 440 450 460 SLDEAVRLAS QEAEAGDTVL LSPACASFDM FSNFETRGKL FKEAVMNL

References

[1]

"Complete sequence of Chloroherpeton thalassium ATCC 35110." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Liu Z., Li T., Zhao F. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35110 / GB-78.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001100 Genomic DNA. Translation: ACF13299.1.
RefSeq	YP_001995746.1. NC_011026.1.
3D structure databases
ProteinModelPortal	B3QWT5.
ModBase	Search...
Protein-protein interaction databases
STRING	B3QWT5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6421883.
GenomeReviews	Gene locus Ctha_0831 in contig CP001100_GR.
KEGG	cts:Ctha_0831.
PATRIC	21430319. VBIChlTha99257_0980.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG750024.
OMA	ACASWDM.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_CHLT3

Accession

Primary (citable) accession number: B3QWT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program