B3QW77 (ISPE_CHLT3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Ctha_0721

Organism

Chloroherpeton thalassium (strain ATCC 35110 / GB-78) [Complete proteome] [HAMAP]

Taxonomic identifier

517418 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chloroherpeton

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000092074
Regions
	Nucleotide binding	92 – 102	11	ATP Potential
Sites
	Active site	10	1	By similarity
	Active site	134	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3QW77 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: DB18A4291FDA9900	FASTA	285	31,225
	10 20 30 40 50 60 MSKTVNAYAK INLALFITGK LPNGYHTLET IFAPINWYDR LTFEPSETIE MACTNAELPT 70 80 90 100 110 120 DDSNLCIKAA KRLQTESGSQ KGVKISLEKN VPFGAGLGGG SSDAAATLNA LNELWELSLP 130 140 150 160 170 180 SETLHKLATE LGADVPYFLE MPALALGTGI GEELTDLQVA FPFSIVTVFP ETAISTAWAY 190 200 210 220 230 240 QNFKQNFDRL LPDAATEIQI VCETGDLGRM QQFENDFESI VYENYAEVKK LRDDFVEAGS 250 260 270 280 GFTRLSGSGS AVFGVFADDE KATACYEAMR KRYPTSLTPK SFKMK

References

[1]

"Complete sequence of Chloroherpeton thalassium ATCC 35110." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Liu Z., Li T., Zhao F. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35110 / GB-78.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001100 Genomic DNA. Translation: ACF13190.1.
RefSeq	YP_001995637.1. NC_011026.1.
3D structure databases
ProteinModelPortal	B3QW77.
ModBase	Search...
Protein-protein interaction databases
STRING	B3QW77.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6421766.
GenomeReviews	Gene locus Ctha_0721 in contig CP001100_GR.
KEGG	cts:Ctha_0721.
PATRIC	21430041. VBIChlTha99257_0848.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	VSTAEVY.
ProtClustDB	PRK14616.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_CHLT3

Accession

Primary (citable) accession number: B3QW77

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program