B3QUJ8 (B3QUJ8_CHLT3) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

Ctha_0433

Organism

Chloroherpeton thalassium (strain ATCC 35110 / GB-78) [Complete proteome] [HAMAP]

Taxonomic identifier

517418 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chloroherpeton

Protein attributes

Sequence length	803 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B3QUJ8 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: D71153260BB0167F	FASTA	803	90,316
	10 20 30 40 50 60 MPAKKSTTGN VGSAKGKKLV IVESPSKAKT INKYLGKDFA VYASVGHIKD LPKKEIGLDF 70 80 90 100 110 120 EHHYEPRYEV IEGKEKVVRE MKKLAGEADE IMIATDPDRE GEAIAWHIAN EVSGAKKPIR 130 140 150 160 170 180 RVLFNEITAR AVKEAIQETR DIDYSLVRSQ QARQAMDKIV GYRISPFLWD TVLRGLSAGR 190 200 210 220 230 240 VQSVALRLIC EREAEIDAFE TKEYWSILAD FTTESGEKFT AKLVKQNGKD IEIPNEAEAK 250 260 270 280 290 300 RLLDLLNARL YAISEIRRRK TKRNPPAPFT TSLLQQSASN RLGYGSKKTM SLAQQLYEGI 310 320 330 340 350 360 EIGSEGAVGL ITYMRTDSKR ISQDAQEEAR KFIQQAFGED YTPEKPKQYK TSEKAQDAHE 370 380 390 400 410 420 AIRPTSVFRK PSDLAPFLSK DQLRLYELIW KRFVASQMSP AELEQTGVDI SDHEKEFIFR 430 440 450 460 470 480 ASGSVVLFDG FLKIFGDQRE LDYEERKSTK EEASEEDAPT KLPKNLKEKE SLTLDALKEN 490 500 510 520 530 540 QHFTKPPARY SEASLVKELD NYGIGRPSTY ASIISTLLDR KYVELRQRRL NPTELGKDVS 550 560 570 580 590 600 RILIANFPEL FNVTFTAGME NNLDKVAAGD DDYESLLDNF YKPLEETLGV RKANPILPQN 610 620 630 640 650 660 ENAEVCDKCG EGRMVVKWTK SGKFLGCSRY PKCKNIKPIA VEKPKPVETG IKCSKCESGR 670 680 690 700 710 720 MVVREGRFGK FLACTNYPEC ESLLNINKKG QVEPPKVPPL TTELACPNCG APLYLREGKR 730 740 750 760 770 780 GLWLGCSKFP KCRGRKSWND LDDETRAKLE AAHSANLAKH PPVEIKMLDG SPLNLNAKLE 790 800 DLISNASDFV KGIEESAEAE IDS

References

[1]

"Complete sequence of Chloroherpeton thalassium ATCC 35110." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Liu Z., Li T., Zhao F. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35110 / GB-78.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001100 Genomic DNA. Translation: ACF12904.1.
RefSeq	YP_001995351.1. NC_011026.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3QUJ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6421476.
GenomeReviews	Gene locus Ctha_0433 in contig CP001100_GR.
KEGG	cts:Ctha_0433.
PATRIC	21429347. VBIChlTha99257_0503.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	EEYWSID.
ProtClustDB	PRK06599.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3QUJ8_CHLT3

Accession

Primary (citable) accession number: B3QUJ8

Entry history

Integrated into UniProtKB/TrEMBL:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)