B3QSP3 (ILVD_CHLT3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Ctha_1632

Organism

Chloroherpeton thalassium (strain ATCC 35110 / GB-78) [Complete proteome] [HAMAP]

Taxonomic identifier

517418 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chloroherpeton

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000089375
Sites
	Metal binding	121	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	194	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	B3QSP3 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: 248541FFEFBD7899	FASTA	560	59,118
	10 20 30 40 50 60 MRSDKIKLGF DKAPHRSLLK ATGAIQSDED FQKPFIGIAN SYIDIIPGHV HLHDFAKRIK 70 80 90 100 110 120 EAVRKAGGIP FEFNTIGVDD GIAMGHIGMR YSLASRELIA DAVETVVGAH WLDAMICIPN 130 140 150 160 170 180 CDKIVPGMMM AAVRLDIPTI FISGGPMKVG VMADGEKVDL ISVFEGVGEY SSGKIDDARL 190 200 210 220 230 240 KELENNGCPT CGSCSGMFTA NSMNCLAEAL GLALPGNGTI LAVDPKREAL AEAAAKQIMT 250 260 270 280 290 300 LVEKDIKPSD LLSRESFLNA FALDLAMGGS TNTILHTLAI ANEAGINFDL AELNTLAAKT 310 320 330 340 350 360 PYICKVSPAT KNVHIEDVDR AGGISAILKE LSKLDGVLDL SRPTVTGKTL GENIADAEVK 370 380 390 400 410 420 DKAVIHSVED PYSATGGLAV LFGNLAPQGC VVKTGAVDPK MMQHTGPARI YESQDEAISG 430 440 450 460 470 480 ILKGDVQAGE VVVIRYEGPK GGPGMPEMLA PTSTIMGQGL GDKVALITDG RFSGGTRGAC 490 500 510 520 530 540 IGHVSPEAAE RGPIAALQNG DKITIDIPNK RIAVDLSDEE IEARLKALPP FEPKIKRGYL 550 560 ARYSQMVTSA GSGAVLKSFD

References

[1]

"Complete sequence of Chloroherpeton thalassium ATCC 35110." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Liu Z., Li T., Zhao F. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35110 / GB-78.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001100 Genomic DNA. Translation: ACF14090.1.
RefSeq	YP_001996537.1. NC_011026.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3QSP3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6422708.
GenomeReviews	Gene locus Ctha_1632 in contig CP001100_GR.
KEGG	cts:Ctha_1632.
PATRIC	21432235. VBIChlTha99257_1915.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK00911.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_CHLT3

Accession

Primary (citable) accession number: B3QSP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program