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B3QS43 (RLMN_CHLT3) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable dual-specificity RNA methyltransferase RlmN

EC=2.1.1.-
EC=2.1.1.192
Alternative name(s):
23S rRNA (adenine(2503)-C(2))-methyltransferase
23S rRNA m2A2503 methyltransferase
Ribosomal RNA large subunit methyltransferase N
tRNA (adenine(37)-C(2))-methyltransferase
tRNA m2A37 methyltransferase
Gene names
Name:rlmN
Ordered Locus Names:Ctha_1529
OrganismChloroherpeton thalassium (strain ATCC 35110 / GB-78) [Complete proteome] [HAMAP]
Taxonomic identifier517418 [NCBI]
Taxonomic lineageBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChloroherpeton

Protein attributes

Sequence length369 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs By similarity. HAMAP-Rule MF_01849

Catalytic activity

2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(2503) in 23S rRNA. HAMAP-Rule MF_01849

2 S-adenosyl-L-methionine + adenine37 in tRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine37 in tRNA. HAMAP-Rule MF_01849

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01849.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue By similarity.

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 369369Probable dual-specificity RNA methyltransferase RlmN HAMAP-Rule MF_01849
PRO_1000188560

Regions

Region171 – 1722S-adenosyl-L-methionine binding By similarity
Region227 – 2293S-adenosyl-L-methionine binding By similarity

Sites

Active site981Proton acceptor Potential
Active site3461S-methylcysteine intermediate By similarity
Metal binding1201Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1241Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1271Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Binding site2041S-adenosyl-L-methionine By similarity
Binding site3031S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity

Amino acid modifications

Disulfide bond113 ↔ 346(transient) By similarity

Sequences

Sequence LengthMass (Da)Tools
B3QS43 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: 81EDE70188B3A8A1

FASTA36941,258
        10         20         30         40         50         60 
MNEVKHDIKA LSLEALMALI NSYGQPAFRA KQIFHWIYAH GVTDFAQMKN LSASFQTLLS 

        70         80         90        100        110        120 
QHFTVSSIQP HADTVSHEIT PEQTVKFLFR LSDEQSIESV FIPSDSTSRN TLCISSQVGC 

       130        140        150        160        170        180 
AFACKFCATG YMGFIRNLTI GEILDQVLWV NRWLGDQRGG KITNVVFMGM GEPLANFDNC 

       190        200        210        220        230        240 
LAAIRILTNP DYAFQISTRK ITVSTVGFIP GIQRLIDTGI NCKLAISLHS AHQAIREELI 

       250        260        270        280        290        300 
PIAKEYSLAT LKAILTRYNQ AYKQPITFEY SLIHKINDSE QDAILLSKFC KGINCKINLI 

       310        320        330        340        350        360 
DYNSVDNIDY LPSPEGHKQA FIRKCIEHGL TVTVRKSRGA DIQAACGQLA IQHVHGKKFS 


KIQTSRHST 

« Hide

References

[1]"Complete sequence of Chloroherpeton thalassium ATCC 35110."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Liu Z., Li T., Zhao F. expand/collapse author list , Overmann J., Bryant D.A., Richardson P.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35110 / GB-78.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001100 Genomic DNA. Translation: ACF13988.1.
RefSeqYP_001996435.1. NC_011026.1.

3D structure databases

ProteinModelPortalB3QS43.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING517418.Ctha_1529.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACF13988; ACF13988; Ctha_1529.
GeneID6422601.
KEGGcts:Ctha_1529.
PATRIC21431979. VBIChlTha99257_1791.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0820.
HOGENOMHOG000217992.
KOK06941.
OMARATICVS.
OrthoDBEOG6DJZ2N.

Enzyme and pathway databases

BioCycCTHA517418:GHTO-1561-MONOMER.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01849. RNA_methyltr_RlmN.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR027492. RNA_MTrfase_RlmN.
IPR004383. rRNA_lsu_MTrfase_RlmN/Cfr.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR30544. PTHR30544. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF006004. CHP00048. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00048. TIGR00048. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRLMN_CHLT3
AccessionPrimary (citable) accession number: B3QS43
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 2, 2008
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families