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B3QS43

- RLMN_CHLT3

UniProt

B3QS43 - RLMN_CHLT3

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Protein
Probable dual-specificity RNA methyltransferase RlmN
Gene
rlmN, Ctha_1529
Organism
Chloroherpeton thalassium (strain ATCC 35110 / GB-78)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs By similarity.UniRule annotation

Catalytic activityi

2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(2503) in 23S rRNA.UniRule annotation
2 S-adenosyl-L-methionine + adenine(37) in tRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei98 – 981Proton acceptor Reviewed prediction
Metal bindingi120 – 1201Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi124 – 1241Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi127 – 1271Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Binding sitei204 – 2041S-adenosyl-L-methionine By similarity
Binding sitei303 – 3031S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity
Active sitei346 – 3461S-methylcysteine intermediate By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
  3. rRNA (adenine-C2-)-methyltransferase activity Source: UniProtKB-HAMAP
  4. rRNA binding Source: UniProtKB-HAMAP
  5. tRNA (adenine-C2-)-methyltransferase activity Source: UniProtKB-HAMAP
  6. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. rRNA base methylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing, tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCTHA517418:GHTO-1561-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dual-specificity RNA methyltransferase RlmN (EC:2.1.1.-, EC:2.1.1.192)
Alternative name(s):
23S rRNA (adenine(2503)-C(2))-methyltransferase
23S rRNA m2A2503 methyltransferase
Ribosomal RNA large subunit methyltransferase N
tRNA (adenine(37)-C(2))-methyltransferase
tRNA m2A37 methyltransferase
Gene namesi
Name:rlmN
Ordered Locus Names:Ctha_1529
OrganismiChloroherpeton thalassium (strain ATCC 35110 / GB-78)
Taxonomic identifieri517418 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChloroherpeton
ProteomesiUP000001208: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Probable dual-specificity RNA methyltransferase RlmNUniRule annotation
PRO_1000188560Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi113 ↔ 346(transient) By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi517418.Ctha_1529.

Structurei

3D structure databases

ProteinModelPortaliB3QS43.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni171 – 1722S-adenosyl-L-methionine binding By similarity
Regioni227 – 2293S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0820.
HOGENOMiHOG000217992.
KOiK06941.
OMAiRATICVS.
OrthoDBiEOG6DJZ2N.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01849. RNA_methyltr_RlmN.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR027492. RNA_MTrfase_RlmN.
IPR004383. rRNA_lsu_MTrfase_RlmN/Cfr.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR30544. PTHR30544. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF006004. CHP00048. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00048. TIGR00048. 1 hit.

Sequencei

Sequence statusi: Complete.

B3QS43-1 [UniParc]FASTAAdd to Basket

« Hide

MNEVKHDIKA LSLEALMALI NSYGQPAFRA KQIFHWIYAH GVTDFAQMKN    50
LSASFQTLLS QHFTVSSIQP HADTVSHEIT PEQTVKFLFR LSDEQSIESV 100
FIPSDSTSRN TLCISSQVGC AFACKFCATG YMGFIRNLTI GEILDQVLWV 150
NRWLGDQRGG KITNVVFMGM GEPLANFDNC LAAIRILTNP DYAFQISTRK 200
ITVSTVGFIP GIQRLIDTGI NCKLAISLHS AHQAIREELI PIAKEYSLAT 250
LKAILTRYNQ AYKQPITFEY SLIHKINDSE QDAILLSKFC KGINCKINLI 300
DYNSVDNIDY LPSPEGHKQA FIRKCIEHGL TVTVRKSRGA DIQAACGQLA 350
IQHVHGKKFS KIQTSRHST 369
Length:369
Mass (Da):41,258
Last modified:September 2, 2008 - v1
Checksum:i81EDE70188B3A8A1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001100 Genomic DNA. Translation: ACF13988.1.
RefSeqiYP_001996435.1. NC_011026.1.

Genome annotation databases

EnsemblBacteriaiACF13988; ACF13988; Ctha_1529.
GeneIDi6422601.
KEGGicts:Ctha_1529.
PATRICi21431979. VBIChlTha99257_1791.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001100 Genomic DNA. Translation: ACF13988.1 .
RefSeqi YP_001996435.1. NC_011026.1.

3D structure databases

ProteinModelPortali B3QS43.
ModBasei Search...

Protein-protein interaction databases

STRINGi 517418.Ctha_1529.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACF13988 ; ACF13988 ; Ctha_1529 .
GeneIDi 6422601.
KEGGi cts:Ctha_1529.
PATRICi 21431979. VBIChlTha99257_1791.

Phylogenomic databases

eggNOGi COG0820.
HOGENOMi HOG000217992.
KOi K06941.
OMAi RATICVS.
OrthoDBi EOG6DJZ2N.

Enzyme and pathway databases

BioCyci CTHA517418:GHTO-1561-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01849. RNA_methyltr_RlmN.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR027492. RNA_MTrfase_RlmN.
IPR004383. rRNA_lsu_MTrfase_RlmN/Cfr.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR30544. PTHR30544. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF006004. CHP00048. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00048. TIGR00048. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Chloroherpeton thalassium ATCC 35110."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Liu Z., Li T., Zhao F.
    , Overmann J., Bryant D.A., Richardson P.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35110 / GB-78.

Entry informationi

Entry nameiRLMN_CHLT3
AccessioniPrimary (citable) accession number: B3QS43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 2, 2008
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue By similarity.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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