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B3QRU2 (SYL_CHLT3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Ctha_1432
OrganismChloroherpeton thalassium (strain ATCC 35110 / GB-78) [Complete proteome] [HAMAP]
Taxonomic identifier517418 [NCBI]
Taxonomic lineageBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChloroherpeton

Protein attributes

Sequence length805 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 805805Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091306

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B3QRU2 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: BF8D0259AE8672C4

FASTA80591,951
        10         20         30         40         50         60 
MKYDFTEIEK KWQAYWEQHQ TFKATEEPNK PKYYVLDMFP YPSGSGLHVG HLEGYTASDI 

        70         80         90        100        110        120 
TARYKRLKGF SVLHPMGWDA FGLPAEQYAI KTGTHPRLTT ETNVANFKST LQRMGFSYDW 

       130        140        150        160        170        180 
SREINTTDPG YVKWTQWIFL KLYEKGLAYV DDVPVNWCEE LKVVLANEEV DEKVNDGYTV 

       190        200        210        220        230        240 
VRRPLRQWML KITAYAERLL TDLDEVDWPE SVKEMQRNWI GKSVGAELDF YLAGTDEKFR 

       250        260        270        280        290        300 
IFTTRPDTVF GATYMVLSPE HPLLDKITTD EHKSAVAAYR EEAERKSDLE RTGLQKEKTG 

       310        320        330        340        350        360 
VFTGSYALNP ATGKEIPIWT SDFVLMGYGT GAIMSVPAHD ERDWEFAKKF GIPIVEVIKS 

       370        380        390        400        410        420 
PHSVQEAVFA GKDSVCTNSE NAEISINGLA YEEAFEKITD WFEKKGLGQR KVNYKLRDWL 

       430        440        450        460        470        480 
FSRQRYWGEP IPIKHYEDGA ERAETELPLN LPEVSAYQPS GTGESPLANI AEWLYGEDEH 

       490        500        510        520        530        540 
GKFRRETNTM PQWAGSCWYY LRFIDPDNPN EAVSPEKEKY WMNVDLYIGG AEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKVLYD VGVVSTKEPF RKLFNQGMIL GEDNEKMSKS RGNVITADSV MSGYGADAVR 

       610        620        630        640        650        660 
LYEMFLGPLE QVKPWSTNGI EGISRFLGKV WRLVYPNEAD GTVQVTDDAP SETVLRRMHK 

       670        680        690        700        710        720 
TIKKVGEDTE TLKFNTAISE MMIYVNELQK EKCRSRTAIE NLLLMLSPFA PHICEELWQA 

       730        740        750        760        770        780 
LGHEESITFA KFPEYDATLA KDDVVTIAVQ VNGKLRGTFD AAAGLSKDDM IAEAMKVESV 

       790        800 
IKFTEGKEIV KQIAVPNKLV NLVVK 

« Hide

References

[1]"Complete sequence of Chloroherpeton thalassium ATCC 35110."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Liu Z., Li T., Zhao F. expand/collapse author list , Overmann J., Bryant D.A., Richardson P.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35110 / GB-78.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001100 Genomic DNA. Translation: ACF13895.1.
RefSeqYP_001996342.1. NC_011026.1.

3D structure databases

ProteinModelPortalB3QRU2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING517418.Ctha_1432.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACF13895; ACF13895; Ctha_1432.
GeneID6422500.
KEGGcts:Ctha_1432.
PATRIC21431745. VBIChlTha99257_1678.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCTHA517418:GHTO-1460-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_CHLT3
AccessionPrimary (citable) accession number: B3QRU2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 2, 2008
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries