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B3QPY9 (MDH_CHLP8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Malate dehydrogenase
EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:Cpar_1594
OrganismChlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327)) [Complete proteome] [HAMAP]
Taxonomic identifier517417 [NCBI]
Taxonomic lineageBacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum ›

Protein attributes

Sequence length310 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate. HAMAP-Rule MF_00487

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487

Subunit structure

Homotetramer; arranged as a dimer of dimers.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 310310Malate dehydrogenase HAMAP-Rule MF_00487
PRO_0000351516

Regions

Nucleotide binding7 – 126NAD By similarity
Nucleotide binding117 – 1193NAD By similarity

Sites

Active site1741Proton acceptor By similarity
Binding site321NAD By similarity
Binding site811Substrate By similarity
Binding site871Substrate By similarity
Binding site941NAD By similarity
Binding site1191Substrate By similarity
Binding site1501Substrate By similarity

Experimental info

Sequence conflict261R → E AA sequence Ref.2
Sequence conflict391Q → E AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
B3QPY9 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: 5CC5DCDE1CC0C22B

FASTA31033,271
        10         20         30         40         50         60 
MKITVIGAGN VGATTAFRIA DKKLARELVL LDVVEGIPQG KGLDMYETGP VGLFDTKITG 

        70         80         90        100        110        120 
SNDYADTADS DIVIITAGLP RKPGMTREDL LMKNAGIVKE VTDNIMKHSK NPIIIVVSNP 

       130        140        150        160        170        180 
LDIMTHVAWV RSGLPKERVI GMAGVLDAAR FRSFIAMELG VSMQDINACV LGGHGDAMVP 

       190        200        210        220        230        240 
VVKYTTVAGI PISDLLPAET IDKLVERTRN GGAEIVEHLK QGSAFYAPAS SVVEMVESIV 

       250        260        270        280        290        300 
LDRKRVLPCA VGLEGQYGID KTFVGVPVKL GRNGVEQIYE INLDQADLDL LQKSAKIVDE 

       310 
NCKMLESTIG

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence of Chlorobaculum parvum NCIB 8327."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Zhao F., Li T., Liu Z. expand/collapse author list , Overmann J., Bryant D.A., Richardson P.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCIB 8327.
[2]"Malate dehydrogenase from Chlorobium vibrioforme, Chlorobium tepidum, and Heliobacterium gestii: purification, characterization, and investigation of dinucleotide binding by dehydrogenases by use of empirical methods of protein sequence analysis."
Charnock C.B., Refseth U.H., Strevaag R.
J. Bacteriol. 174:1307-1313(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-40.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001099 Genomic DNA. Translation: ACF11992.1.
RefSeqYP_001999192.1. NC_011027.1.

3D structure databases

ProteinModelPortalB3QPY9.
ModBaseSearch...

Protein-protein interaction databases

STRING517417.Cpar_1594.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACF11992; ACF11992; Cpar_1594.
GeneID6420536.
KEGGcpc:Cpar_1594.
PATRIC21366472. VBIChlPar72705_1590.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000213794.
KOK00024.
OMADAMTYVM.
ProtClustDBPRK06223.

Enzyme and pathway databases

BioCycCPAR517417:GH95-1637-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_00487. Malate_dehydrog_3.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMsTIGR01763. MalateDH_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_CHLP8
AccessionPrimary (citable) accession number: B3QPY9
Secondary accession number(s): P80038, P94687
Entry history
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: September 2, 2008
Last modified: May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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