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Protein

Malate dehydrogenase

Gene

mdh

Organism
Chlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32NADUniRule annotation1
Binding sitei81SubstrateUniRule annotation1
Binding sitei87SubstrateUniRule annotation1
Binding sitei94NADUniRule annotation1
Binding sitei119SubstrateUniRule annotation1
Binding sitei150SubstrateUniRule annotation1
Active sitei174Proton acceptorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 12NADUniRule annotation6
Nucleotide bindingi117 – 119NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Cpar_1594
OrganismiChlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327))
Taxonomic identifieri517417 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi
  • UP000008811 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003515161 – 310Malate dehydrogenaseAdd BLAST310

Interactioni

Subunit structurei

Homotetramer; arranged as a dimer of dimers.

Protein-protein interaction databases

STRINGi517417.Cpar_1594.

Structurei

3D structure databases

ProteinModelPortaliB3QPY9.
SMRiB3QPY9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213794.
KOiK00024.
OMAiMDLMQTA.
OrthoDBiPOG091H02N6.

Family and domain databases

CDDicd01339. LDH-like_MDH. 1 hit.
Gene3Di3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3. 1 hit.
InterProiView protein in InterPro
IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR036291. NAD(P)-bd_dom_sf.
PfamiView protein in Pfam
PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

B3QPY9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKITVIGAGN VGATTAFRIA DKKLARELVL LDVVEGIPQG KGLDMYETGP
60 70 80 90 100
VGLFDTKITG SNDYADTADS DIVIITAGLP RKPGMTREDL LMKNAGIVKE
110 120 130 140 150
VTDNIMKHSK NPIIIVVSNP LDIMTHVAWV RSGLPKERVI GMAGVLDAAR
160 170 180 190 200
FRSFIAMELG VSMQDINACV LGGHGDAMVP VVKYTTVAGI PISDLLPAET
210 220 230 240 250
IDKLVERTRN GGAEIVEHLK QGSAFYAPAS SVVEMVESIV LDRKRVLPCA
260 270 280 290 300
VGLEGQYGID KTFVGVPVKL GRNGVEQIYE INLDQADLDL LQKSAKIVDE
310
NCKMLESTIG
Length:310
Mass (Da):33,271
Last modified:September 2, 2008 - v1
Checksum:i5CC5DCDE1CC0C22B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26R → E AA sequence (PubMed:1735722).Curated1
Sequence conflicti39Q → E AA sequence (PubMed:1735722).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001099 Genomic DNA. Translation: ACF11992.1.
RefSeqiWP_012502825.1. NC_011027.1.

Genome annotation databases

EnsemblBacteriaiACF11992; ACF11992; Cpar_1594.
KEGGicpc:Cpar_1594.

Similar proteinsi

Entry informationi

Entry nameiMDH_CHLP8
AccessioniPrimary (citable) accession number: B3QPY9
Secondary accession number(s): P80038, P94687
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: September 2, 2008
Last modified: October 25, 2017
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families