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B3QNE9

- LIPA_CHLP8

UniProt

B3QNE9 - LIPA_CHLP8

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Protein
Lipoyl synthase
Gene
lipA, Cpar_1044
Organism
Chlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi38 – 381Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi43 – 431Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi49 – 491Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi64 – 641Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi68 – 681Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi71 – 711Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCPAR517417:GH95-1075-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Cpar_1044
OrganismiChlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327))
Taxonomic identifieri517417 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
ProteomesiUP000008811: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292Lipoyl synthaseUniRule annotation
PRO_1000099593Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi517417.Cpar_1044.

Structurei

3D structure databases

ProteinModelPortaliB3QNE9.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiPNIAECF.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B3QNE9-1 [UniParc]FASTAAdd to Basket

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MNSGPGKKPD WLKIKLTSGS SFASTKKLLN KHSLHTVCRS AMCPNLHECW    50
SKGTATFLLL GNVCTRSCRF CAVGTERRPA MPDPEETAKI GEAVKAMKLR 100
HAVLTSVNRD DLADGGAAHW VETIRAIREV NPGVSIECLI PDFQGDEQAL 150
DSVMRERPEV LNHNIETVPS RYASVRPQAS YERSLAVIER AKRQFRLATK 200
SGMMVGMGET PEEVNAALRD LRAHGCDMVT IGQYLQPTAT HLPVSRYVTP 250
EEFERYREIA LDAGFRHVQS GPFVRSSYHA EAFEPVEEIS NS 292
Length:292
Mass (Da):32,438
Last modified:September 2, 2008 - v1
Checksum:i88E63870FFD29549
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001099 Genomic DNA. Translation: ACF11452.1.
RefSeqiYP_001998652.1. NC_011027.1.

Genome annotation databases

EnsemblBacteriaiACF11452; ACF11452; Cpar_1044.
GeneIDi6419974.
KEGGicpc:Cpar_1044.
PATRICi21365335. VBIChlPar72705_1034.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001099 Genomic DNA. Translation: ACF11452.1 .
RefSeqi YP_001998652.1. NC_011027.1.

3D structure databases

ProteinModelPortali B3QNE9.
ModBasei Search...

Protein-protein interaction databases

STRINGi 517417.Cpar_1044.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACF11452 ; ACF11452 ; Cpar_1044 .
GeneIDi 6419974.
KEGGi cpc:Cpar_1044.
PATRICi 21365335. VBIChlPar72705_1034.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi PNIAECF.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci CPAR517417:GH95-1075-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCIB 8327.

Entry informationi

Entry nameiLIPA_CHLP8
AccessioniPrimary (citable) accession number: B3QNE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 2, 2008
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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