Reviewed, UniProtKB/Swiss-Prot B3QMF5 (SAHH_CHLP8)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	Cpar_0688

Organism

Chlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327)) [Complete proteome] [HAMAP]

Taxonomic identifier

517417 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Adenosylhomocysteinase HAMAP MF_00563		PRO_1000129276
Regions
	Region	223 – 391	169	NAD binding By similarity
Sites
	Binding site	60	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	196	1	Substrate By similarity
	Binding site	226	1	Substrate By similarity
	Binding site	230	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3QMF5-1 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: 5C5B17AA210D370A	FASTA	471	52,107
	10 20 30 40 50 60 MTTEAAVLDY KVADISLAEW GRKEIEIAEK EMPGLMATRK KYEGKKPLAG ARIAGSLHMT 70 80 90 100 110 120 IQTAVLIETL VELGADVRWA SCNIFSTQDH AAAAIAAAGV PVFAWKGETL DEYWWCTRQI 130 140 150 160 170 180 LEFEGGLGPN LIVDDGGDAT LMIHLGYKIE NDPSMLDKTP GNAEERALFA QLKEVFAEDN 190 200 210 220 230 240 QRWHKVAAGM KGVSEETTTG VHRLYQMMEK GELLFPAINV NDSVTKSKFD NLYGCRESLA 250 260 270 280 290 300 DGIKRATDVM IAGKVAVVLG YGDVGKGCAH SMRSYGARVI VTEIDPICAL QAAMEGFQVT 310 320 330 340 350 360 TIEEALEEGN IYVTTTGNKD VITLEHMKKM KDEAIVCNIG HFDNEIQVDA LNNFKGATRI 370 380 390 400 410 420 NIKPQVDKYV FEDGRCIYLL AEGRLVNLGC ATGHPSFVMS NSFTNQTLAQ IELWKNNYDV 430 440 450 460 470 DVYRLPKHLD EEVARLHLGQ IGVKLTTLSK EQADYIGVPV EGPYKPEHYR Y

References

[1]

"Complete sequence of Chlorobaculum parvum NCIB 8327." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Zhao F., Li T., Liu Z. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001099 Genomic DNA. Translation: ACF11108.1.
RefSeq	YP_001998308.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6419608.
GenomeReviews	Gene locus Cpar_0688 in contig CP001099_GR.
KEGG	cpc:Cpar_0688.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HMRAMKD.
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_CHLP8

Accession

Primary (citable) accession number: B3QMF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)