B3QMA2 (ILVD_CHLP8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Cpar_0635

Organism

Chlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327)) [Complete proteome] [HAMAP]

Taxonomic identifier

517417 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000089374
Sites
	Metal binding	121	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	194	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	B3QMA2 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: 6C25D19652DDEA44	FASTA	560	58,824
	10 20 30 40 50 60 MRSDTIKKGF EKAPHRSLLK ATGAISTRDD YSKPFIGICN SFNELIPGHA HLQELGRIAK 70 80 90 100 110 120 EAVREAGGVP FEFNTIGVCD GIAMGHVGMR YSLASRELIA DSVETVVEAH RLDGLVCIPN 130 140 150 160 170 180 CDKITPGMMM GALRTNVPVI FVSGGPMSAG HTPSGKTVDL ISVFEAVGQC STGEITEDEL 190 200 210 220 230 240 QTIEECGCPG CGSCSGMFTA NSMNCLCEAL GFALPGNGTI LAADPRRNEL VKAAAGRIVD 250 260 270 280 290 300 LVNKDVRPRS ILSRQSMLNA FALDLAMGGS TNTILHTLAI ASEAELEFDF SELNDLSAKT 310 320 330 340 350 360 PYICKVSPAT TEVHIEDVDR AGGISAILKE LSKVEGLLDL SAPTVTGKTL GENIADAEVL 370 380 390 400 410 420 DRSVIRSVEE PYSATGGLAV LYGNLAPNGS VVKTGAVSPS MMKHTGPAKV YDCQDDAIAG 430 440 450 460 470 480 IMNGDVKSGD VVVIRYEGPR GGPGMPEMLS PTSAIMGRGL GDSVALITDG RFSGGSRGAC 490 500 510 520 530 540 VGHVSPEAAD RGPIAAVQTG DMITIDIPGR TMSVALDDET IRQRIEALPE FEPKIKKGYL 550 560 ARYARMVTSA NTGAVLTTNF

References

[1]

"Complete sequence of Chlorobaculum parvum NCIB 8327." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Zhao F., Li T., Liu Z. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCIB 8327.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001099 Genomic DNA. Translation: ACF11055.1.
RefSeq	YP_001998255.1. NC_011027.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3QMA2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6419553.
GenomeReviews	Gene locus Cpar_0635 in contig CP001099_GR.
KEGG	cpc:Cpar_0635.
PATRIC	21364506. VBIChlPar72705_0632.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK00911.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_CHLP8

Accession

Primary (citable) accession number: B3QMA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program