B3QM51 (NADE_CHLP8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Cpar_0584

Organism

Chlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327)) [Complete proteome] [HAMAP]

Taxonomic identifier

517417 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099015
Regions
	Nucleotide binding	36 – 43	8	ATP By similarity
Sites
	Active site	38	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3QM51 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: 486A6DEFD661A4C2	FASTA	277	31,189
	10 20 30 40 50 60 MEPQDLHLDY GLVESILIPF IRNEIRKFGF RSVVLGLSGG IDSAVVCELA ARSLGADNVL 70 80 90 100 110 120 ALLMPYKTSS RESLEHAQLM VDRLGIRAET MPVTGVVDAF FETRPDASRL RRGNVMARSR 130 140 150 160 170 180 MLCLYDVSAR DGCLVLGTSN KTELMLGYGT MFGDMASAVN PIGDLYKTQL WGLARHLGVP 190 200 210 220 230 240 SELIDKQPSA DLWEGQSDEA DLGFSYEEVD QLLYMMLEER MERDAILAEG IAPAFYDRVR 250 260 270 QMVVRNQYKR MMPVIAKLSS RTPGIDFRYA RDWQEMK

References

[1]

"Complete sequence of Chlorobaculum parvum NCIB 8327." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Zhao F., Li T., Liu Z. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCIB 8327.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001099 Genomic DNA. Translation: ACF11004.1.
RefSeq	YP_001998204.1. NC_011027.1.
3D structure databases
ProteinModelPortal	B3QM51.
ModBase	Search...
Protein-protein interaction databases
STRING	B3QM51.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6419502.
GenomeReviews	Gene locus Cpar_0584 in contig CP001099_GR.
KEGG	cpc:Cpar_0584.
PATRIC	21364406. VBIChlPar72705_0582.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	DGAVDCH.
ProtClustDB	PRK13980.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_CHLP8

Accession

Primary (citable) accession number: B3QM51

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program