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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Chlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei579 – 5791ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPAR517417:GH95-554-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Cpar_0539
OrganismiChlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327))
Taxonomic identifieri517417 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
ProteomesiUP000008811 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 807807Leucine--tRNA ligasePRO_1000091303Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi517417.Cpar_0539.

Structurei

3D structure databases

ProteinModelPortaliB3QM08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi576 – 5805"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

B3QM08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYDFSALEK KWQARWADEQ TFASSADQDK PKYYVLDMFP YPSGSGLHVG
60 70 80 90 100
HLEGYTATDI MARYKRCQGH NVLHPMGWDA FGLPAEQFAI KTGTHPRLTT
110 120 130 140 150
EKNVASFRET LKSMGFSYDW SREVNTTDPN YFKWTQWIFL KLYEKGLAYI
160 170 180 190 200
SEVDVNWCEE LKVVLANEEV DEKIADGYTV VRRPLRQWVL KITAYAERLL
210 220 230 240 250
EDLDEVDWPE NVKQMQRNWI GRSEGVEIDF ELRCHRTNLR VYTTRPDTLF
260 270 280 290 300
GATYLVISPE HPMAEKLAIA QQLVEVKKYI EQAKLKTELE RTGLQKEKTG
310 320 330 340 350
VFTGSYAINP ANGEALPVWI SDFVLTSYGT GAIMSVPAHD SRDWEFAKKF
360 370 380 390 400
GLPIREVIKS PHDVQEEVFD GKESVCVNSA NDEISIDGLD FKTAFDRMAT
410 420 430 440 450
WLESKGKGKR KVNYKLRDWV FSRQRYWGEP IPIKHYEDGT MRPETNLPLT
460 470 480 490 500
LPEVEAYHPT STGESPLANI ESWLYGEDEH GKFRRETNTM PQWAGSCWYY
510 520 530 540 550
LRFIDPQNGN ALVDPSREQY WMNVDLYIGG AEHAVLHLLY SRFWHKVLYD
560 570 580 590 600
LGVVSTKEPF QRLFNQGMIL GEDNEKMSKS RGNVIPADHV LSTYGADAVR
610 620 630 640 650
LYEMFLGPLE QVKPWNTHGI EGISRFLNKV WRLVWDENTE TQKTTDDKPS
660 670 680 690 700
EAVLKRMHKA IKKVTEDTEQ LKFNTAISEM MVLVNELTKT GCYSRETTET
710 720 730 740 750
LLVLLSPFAP HITEELWQTL GHTESISGAV WPVFDAKLAT DDVLTIAVQV
760 770 780 790 800
NGKLRGTFEA PAGYAKEDMI ESAKKVESVA KFLEGQQIIK EIAVPGKLVN

FAVKPQQ
Length:807
Mass (Da):92,556
Last modified:September 2, 2008 - v1
Checksum:iD495F8BEC478107D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001099 Genomic DNA. Translation: ACF10961.1.
RefSeqiWP_012501794.1. NC_011027.1.
YP_001998161.1. NC_011027.1.

Genome annotation databases

EnsemblBacteriaiACF10961; ACF10961; Cpar_0539.
KEGGicpc:Cpar_0539.
PATRICi21364310. VBIChlPar72705_0538.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001099 Genomic DNA. Translation: ACF10961.1.
RefSeqiWP_012501794.1. NC_011027.1.
YP_001998161.1. NC_011027.1.

3D structure databases

ProteinModelPortaliB3QM08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi517417.Cpar_0539.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACF10961; ACF10961; Cpar_0539.
KEGGicpc:Cpar_0539.
PATRICi21364310. VBIChlPar72705_0538.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciCPAR517417:GH95-554-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCIB 8327.

Entry informationi

Entry nameiSYL_CHLP8
AccessioniPrimary (citable) accession number: B3QM08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 2, 2008
Last modified: April 1, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.