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B3QM08 (SYL_CHLP8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Cpar_0539
OrganismChlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327)) [Complete proteome] [HAMAP]
Taxonomic identifier517417 [NCBI]
Taxonomic lineageBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum

Protein attributes

Sequence length807 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 807807Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091303

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B3QM08 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: D495F8BEC478107D

FASTA80792,556
        10         20         30         40         50         60 
MKYDFSALEK KWQARWADEQ TFASSADQDK PKYYVLDMFP YPSGSGLHVG HLEGYTATDI 

        70         80         90        100        110        120 
MARYKRCQGH NVLHPMGWDA FGLPAEQFAI KTGTHPRLTT EKNVASFRET LKSMGFSYDW 

       130        140        150        160        170        180 
SREVNTTDPN YFKWTQWIFL KLYEKGLAYI SEVDVNWCEE LKVVLANEEV DEKIADGYTV 

       190        200        210        220        230        240 
VRRPLRQWVL KITAYAERLL EDLDEVDWPE NVKQMQRNWI GRSEGVEIDF ELRCHRTNLR 

       250        260        270        280        290        300 
VYTTRPDTLF GATYLVISPE HPMAEKLAIA QQLVEVKKYI EQAKLKTELE RTGLQKEKTG 

       310        320        330        340        350        360 
VFTGSYAINP ANGEALPVWI SDFVLTSYGT GAIMSVPAHD SRDWEFAKKF GLPIREVIKS 

       370        380        390        400        410        420 
PHDVQEEVFD GKESVCVNSA NDEISIDGLD FKTAFDRMAT WLESKGKGKR KVNYKLRDWV 

       430        440        450        460        470        480 
FSRQRYWGEP IPIKHYEDGT MRPETNLPLT LPEVEAYHPT STGESPLANI ESWLYGEDEH 

       490        500        510        520        530        540 
GKFRRETNTM PQWAGSCWYY LRFIDPQNGN ALVDPSREQY WMNVDLYIGG AEHAVLHLLY 

       550        560        570        580        590        600 
SRFWHKVLYD LGVVSTKEPF QRLFNQGMIL GEDNEKMSKS RGNVIPADHV LSTYGADAVR 

       610        620        630        640        650        660 
LYEMFLGPLE QVKPWNTHGI EGISRFLNKV WRLVWDENTE TQKTTDDKPS EAVLKRMHKA 

       670        680        690        700        710        720 
IKKVTEDTEQ LKFNTAISEM MVLVNELTKT GCYSRETTET LLVLLSPFAP HITEELWQTL 

       730        740        750        760        770        780 
GHTESISGAV WPVFDAKLAT DDVLTIAVQV NGKLRGTFEA PAGYAKEDMI ESAKKVESVA 

       790        800 
KFLEGQQIIK EIAVPGKLVN FAVKPQQ 

« Hide

References

[1]"Complete sequence of Chlorobaculum parvum NCIB 8327."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Zhao F., Li T., Liu Z. expand/collapse author list , Overmann J., Bryant D.A., Richardson P.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCIB 8327.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001099 Genomic DNA. Translation: ACF10961.1.
RefSeqYP_001998161.1. NC_011027.1.

3D structure databases

ProteinModelPortalB3QM08.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING517417.Cpar_0539.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACF10961; ACF10961; Cpar_0539.
GeneID6419453.
KEGGcpc:Cpar_0539.
PATRIC21364310. VBIChlPar72705_0538.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAERYWMNV.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCPAR517417:GH95-554-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_CHLP8
AccessionPrimary (citable) accession number: B3QM08
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 2, 2008
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries