B3QKU1 (MASZ_RHOPT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	Rpal_4697

Organism

Rhodopseudomonas palustris (strain TIE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

395960 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity. HAMAP MF_00641
Subcellular location	Cytoplasm By similarity HAMAP MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 724	724	Malate synthase G HAMAP MF_00641		PRO_1000130897
Sites
	Active site	337	1	Proton acceptor By similarity
	Active site	628	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3QKU1 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: 0102198CFF95EEFC	FASTA	724	78,535
	10 20 30 40 50 60 MNRIDAHGLK IAPVLFDFIA KEAAPKTGIA PDVFWAGLAA IVRDLAPKTR ALLKTRDDLQ 70 80 90 100 110 120 AKIDAWHLAN KGKKQDMAAY TAFLKEIGYL LPEPPTVPVE TANIDEEIGK LCGPQLVVPL 130 140 150 160 170 180 SNARYALNAA NARWGSLYDA FYGTDAIPQE ATQAKGYDKA RGDKVIAKAK AFLDQAAPLA 190 200 210 220 230 240 TGSHSDVTGY SVIAGQLSAK LKSGNATGLK KPAQFAGFRG DAANPSAVLL VNNGLHIEIK 250 260 270 280 290 300 IDRANTIGKD DPAGVADLVI ESAVSTILDM EDSVAAVDAD DKVLIYRNTL GLMDGTLSES 310 320 330 340 350 360 FEKGGKTVTR ALNGDRTYTA PDGKEISLHG RSLLLMRNVG HHMWTDAVLD SDGQEIPEGF 370 380 390 400 410 420 LDAAVSGLIA IHDLKHLGKT RNSRTGSVYI VKPKMHGPDE VALTVELFGR VETMLGLTAN 430 440 450 460 470 480 TLKVGIMDEE RRTTVNLKAC IQNASKRIVF INTGFLDRTG DEIHTSMEAG PMIRKNEMKA 490 500 510 520 530 540 QPWIKAYEDW NVDTGLVDGL PGHAQIGKGM WAAPDKMADM LAQKIGHPQA GATTAWVPSP 550 560 570 580 590 600 TAATLHALHY HQVDVIARQQ ELAKGGPRAK LEDILTIPVS NSNWAPDDVR QEIDNNCQGI 610 620 630 640 650 660 LGYVVRWIDQ GVGCSKVPDI HDVGLMEDRA TLRISSQHLA NWLHHGVVTK DQVLDSLKRM 670 680 690 700 710 720 AVIVDKQNEG DALYRPIAPD FDGVAFEAAC DLIFKGRAQP NGYTEYILHE RRREAKAAHL ESAR

References

[1]

"Complete sequence of Rhodopseudomonas palustris TIE-1." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Emerson D., Newman D.K., Roden E., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TIE-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001096 Genomic DNA. Translation: ACF03188.1.
RefSeq	YP_001993663.1. NC_011004.1.
3D structure databases
ProteinModelPortal	B3QKU1.
SMR	B3QKU1. Positions 9-721.
ModBase	Search...
Protein-protein interaction databases
STRING	B3QKU1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6412383.
GenomeReviews	Gene locus Rpal_4697 in contig CP001096_GR.
KEGG	rpt:Rpal_4697.
PATRIC	23313936. VBIRhoPal88240_4755.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG350005.
OMA	PKMHGPD.
ProtClustDB	PRK02999.
Family and domain databases
HAMAP	MF_00641. Malate_synth_G. [Tree]
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
Gene3D	G3DSA:2.170.170.11. Malate_synth_G_beta_sub_dom. 2 hits.
KO	K01638.
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. Malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_RHOPT

Accession

Primary (citable) accession number: B3QKU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program