B3QIG6 (LPXK_RHOPT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	Rpal_1350

Organism

Rhodopseudomonas palustris (strain TIE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

395960 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_1000123734
Regions
	Nucleotide binding	51 – 58	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B3QIG6 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: EC50E355A627FB5C	FASTA	340	35,594
	10 20 30 40 50 60 MREPGFWHRP PSLVSRLLLP IAAIYGNIAA ARMQKAGTTV GVPVLCVGNY HMGGAGKTPT 70 80 90 100 110 120 TLALVALLRE FGETPVVLSR GYGGRLQGPV QVDPSRHSAA DIGDEPLMMA RRLAVVVARD 130 140 150 160 170 180 RTDGAALACA LGATVILMDD GFQNPALTKD ASLIVVDSHR SIGNGSVFPA GPLRAPLPLQ 190 200 210 220 230 240 VARTDALVVV GDGAAADGLA QQITTKGGVV LRARLVPEPA SVEALRGRRV LAFAGIGDPA 250 260 270 280 290 300 RFVATLRGSG VEVVEQRAFA DHHPFTAEEL AELAAAAKRD GLTLVTTEKD LARIGRAQQA 310 320 330 340 LGVEIVPFAV TLAFGDEAKL RLFLLDRLNG ARAAKLAGRR

References

[1]

"Complete sequence of Rhodopseudomonas palustris TIE-1." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Emerson D., Newman D.K., Roden E., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TIE-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001096 Genomic DNA. Translation: ACE99889.1.
RefSeq	YP_001990365.1. NC_011004.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3QIG6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6409007.
GenomeReviews	Gene locus Rpal_1350 in contig CP001096_GR.
KEGG	rpt:Rpal_1350.
PATRIC	23307119. VBIRhoPal88240_1378.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG663914.
OMA	VILMDDG.
ProtClustDB	PRK00652.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_RHOPT

Accession

Primary (citable) accession number: B3QIG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program