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Protein

Ribonuclease E

Gene

rne

Organism
Rhodopseudomonas palustris (strain TIE-1)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
  • Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per homotetramer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi544Magnesium; catalyticUniRule annotation1
Metal bindingi587Magnesium; catalyticUniRule annotation1
Metal bindingi645Zinc; shared with dimeric partnerUniRule annotation1
Metal bindingi648Zinc; shared with dimeric partnerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

EndonucleaseUniRule annotation, Hydrolase, Nuclease

Keywords - Biological processi

rRNA processingUniRule annotation, tRNA processingUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation, RNA-bindingUniRule annotation, ZincUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease EUniRule annotation (EC:3.1.26.12UniRule annotation)
Short name:
RNase EUniRule annotation
Gene namesi
Name:rneUniRule annotation
Ordered Locus Names:Rpal_2728Imported
OrganismiRhodopseudomonas palustris (strain TIE-1)Imported
Taxonomic identifieri395960 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
Proteomesi
  • UP000001725 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membraneUniRule annotation, Cell membrane, CytoplasmUniRule annotation, Membrane

Interactioni

Subunit structurei

Homotetramer formed by a dimer of dimers.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB3QH48.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni645 – 648Required for zinc-mediated homotetramerization and catalytic activityUniRule annotation4

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili83 – 110Sequence analysisAdd BLAST28

Sequence similaritiesi

Belongs to the RNase E/G family. RNase E subfamily.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

HOGENOMiHOG000258026.
KOiK08300.
OMAiPFGLLEM.
OrthoDBiPOG091H05KQ.

Family and domain databases

HAMAPiMF_00970. RNase_E. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR019307. RNA-bd_AU-1/RNase_E/G.
IPR028878. RNase_E.
IPR004659. RNase_E/G.
[Graphical view]
PfamiPF10150. RNase_E_G. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00757. RNaseEG. 1 hit.

Sequencei

Sequence statusi: Complete.

B3QH48-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANKMLIDAT HPEETRVVVV RGNRVEEFDF ETAQRKQLRG NIYLAKVTRV
60 70 80 90 100
EPSLQAAFIE YGGNRHGFLA FSEIHPDYYQ IPVADRQALI EADERAHREA
110 120 130 140 150
EEENEQRSSR RRSRHRSHRR RNNGERVQSE IVEAATDVAA EQPHEAADHH
160 170 180 190 200
EAHEAGAHAE AERLMHELPE GETAEQHDAE YAADHEHTHV EAAPSEPQVS
210 220 230 240 250
AAAEPVTIEQ PAAAEAPAPV EAPQAAASHD ETDAHDDDHG EDSANYAEDA
260 270 280 290 300
PFPIDEHHEA EDEHEAHDDH VEGEHEVETD SDSDTEAEAE DDEEEDDEEE
310 320 330 340 350
AEEDVVESVG GDDVLEEVPE RAFRPRRQYK IQEVIKRRQV MLVQVVKEER
360 370 380 390 400
GNKGAALTTY LSLAGRYAVL MPNTARGGGI SRKITSAQDR SRLKEVVQDL
410 420 430 440 450
DVPEGMGVIL RTAGASRTKP EIKRDFEYLI RMWETVRDVT LNSQAPKLVY
460 470 480 490 500
EEGSLIKRSL RDLYNKEIDE VLVAGEAGYR EARDFMHMLM PSQVRAVKLY
510 520 530 540 550
RDGQPLFSRM GVESQLDAMF SPTVQLRSGG YIVINQTEAL VSIDVNSGRS
560 570 580 590 600
TREHHIEDTA LKTNMEAAEE VARQLRLRDL AGLIVIDFID MDEKRNNRAV
610 620 630 640 650
ERKLSDCLRQ DRARIQVGRI SHFGLLEMSR QRIRASVLES STEPCPHCGG
660 670 680 690 700
SGHVRSVSSV ALQLLRGLEE VLMKGATHNL IVRTRTDVAL YVLNHKRGHL
710 720 730 740 750
RDLETGFRVS LSVLADPTVS GQQSFVIDRG EQVHTLETAK ALLAAQIAAS
760 770 780 790 800
PAPLDEPEDD DGYDFEAEIE TDETVGLAED QGSEAGESES EGRKRRRRRR
810 820 830 840 850
RRRSGEPREA AEAGAVGEDG EQPFVSEGVE QVGEADEGDE AGEDDSDAEA
860 870 880 890 900
RADQGENGER RPRRRGRRGG RRRRGNAEAG ENEAGVVGSI SDELSPTDEA
910 920 930 940 950
EAADAVADFD GGSPSVQPSF EPAAEAEQPH DAPPHDIVPS EPQHTAATAA
960 970 980 990 1000
PDTSDPSAED DSAAKKAARR RSTVREKVSF GASAAASETT AEAAAEVQPE
1010 1020 1030 1040 1050
PTAQAEQPAT AAPAVEAPAA EAAPQAAAQP AEEPSSAAPR RVGWWSRRFG

GGN
Length:1,053
Mass (Da):115,731
Last modified:September 2, 2008 - v1
Checksum:i630391D431EEC8AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001096 Genomic DNA. Translation: ACF01237.1.
RefSeqiWP_012495937.1. NC_011004.1.

Genome annotation databases

EnsemblBacteriaiACF01237; ACF01237; Rpal_2728.
KEGGirpt:Rpal_2728.
PATRICi23309912. VBIRhoPal88240_2766.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001096 Genomic DNA. Translation: ACF01237.1.
RefSeqiWP_012495937.1. NC_011004.1.

3D structure databases

ProteinModelPortaliB3QH48.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACF01237; ACF01237; Rpal_2728.
KEGGirpt:Rpal_2728.
PATRICi23309912. VBIRhoPal88240_2766.

Phylogenomic databases

HOGENOMiHOG000258026.
KOiK08300.
OMAiPFGLLEM.
OrthoDBiPOG091H05KQ.

Family and domain databases

HAMAPiMF_00970. RNase_E. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR019307. RNA-bd_AU-1/RNase_E/G.
IPR028878. RNase_E.
IPR004659. RNase_E/G.
[Graphical view]
PfamiPF10150. RNase_E_G. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00757. RNaseEG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB3QH48_RHOPT
AccessioniPrimary (citable) accession number: B3QH48
Entry historyi
Integrated into UniProtKB/TrEMBL: September 2, 2008
Last sequence update: September 2, 2008
Last modified: November 2, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.