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B3QH48

- B3QH48_RHOPT

UniProt

B3QH48 - B3QH48_RHOPT

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Protein

Ribonuclease E

Gene

rne

Organism
Rhodopseudomonas palustris (strain TIE-1)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.UniRule annotation

Cofactori

Binds 1 Mg2+ ion per subunit.UniRule annotation
Binds 2 Zn2+ ions per homotetramer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi544 – 5441Magnesium; catalyticUniRule annotation
Metal bindingi587 – 5871Magnesium; catalyticUniRule annotation
Metal bindingi645 – 6451Zinc; shared with dimeric partnerUniRule annotation
Metal bindingi648 – 6481Zinc; shared with dimeric partnerUniRule annotation

GO - Molecular functioni

  1. endoribonuclease activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. ribonuclease E activity Source: InterPro
  4. RNA binding Source: UniProtKB-HAMAP
  5. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. rRNA processing Source: UniProtKB-HAMAP
  3. tRNA processing Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

EndonucleaseUniRule annotation, Hydrolase, Nuclease

Keywords - Biological processi

rRNA processingUniRule annotation, tRNA processingUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation, RNA-bindingUniRule annotation, ZincUniRule annotation

Enzyme and pathway databases

BioCyciRPAL395960:GHPC-2750-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease EUniRule annotation (EC:3.1.26.12UniRule annotation)
Short name:
RNase EUniRule annotation
Gene namesi
Name:rneUniRule annotation
Ordered Locus Names:Rpal_2728Imported
OrganismiRhodopseudomonas palustris (strain TIE-1)Imported
Taxonomic identifieri395960 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
ProteomesiUP000001725: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation. Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. cytoplasmic side of plasma membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membraneUniRule annotation, Cell membrane, CytoplasmUniRule annotation, Membrane

Interactioni

Subunit structurei

Homotetramer formed by a dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi395960.Rpal_2728.

Structurei

3D structure databases

ProteinModelPortaliB3QH48.
SMRiB3QH48. Positions 36-80.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 11172S1 motifUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni645 – 6484Required for zinc-mediated homotetramerization and catalytic activityUniRule annotation

Sequence similaritiesi

Belongs to the RNase E/G family. RNase E subfamily.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1530.
HOGENOMiHOG000258026.
KOiK08300.
OMAiPFGLLEM.
OrthoDBiEOG6PCPTH.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
HAMAPiMF_00970. RNase_E.
InterProiIPR012340. NA-bd_OB-fold.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR019307. RNA-bd_AU-1/RNase_E/G.
IPR028878. RNase_E.
IPR004659. RNase_E/G.
IPR022967. S1_dom.
[Graphical view]
PfamiPF10150. RNase_E_G. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00757. RNaseEG. 1 hit.

Sequencei

Sequence statusi: Complete.

B3QH48 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANKMLIDAT HPEETRVVVV RGNRVEEFDF ETAQRKQLRG NIYLAKVTRV
60 70 80 90 100
EPSLQAAFIE YGGNRHGFLA FSEIHPDYYQ IPVADRQALI EADERAHREA
110 120 130 140 150
EEENEQRSSR RRSRHRSHRR RNNGERVQSE IVEAATDVAA EQPHEAADHH
160 170 180 190 200
EAHEAGAHAE AERLMHELPE GETAEQHDAE YAADHEHTHV EAAPSEPQVS
210 220 230 240 250
AAAEPVTIEQ PAAAEAPAPV EAPQAAASHD ETDAHDDDHG EDSANYAEDA
260 270 280 290 300
PFPIDEHHEA EDEHEAHDDH VEGEHEVETD SDSDTEAEAE DDEEEDDEEE
310 320 330 340 350
AEEDVVESVG GDDVLEEVPE RAFRPRRQYK IQEVIKRRQV MLVQVVKEER
360 370 380 390 400
GNKGAALTTY LSLAGRYAVL MPNTARGGGI SRKITSAQDR SRLKEVVQDL
410 420 430 440 450
DVPEGMGVIL RTAGASRTKP EIKRDFEYLI RMWETVRDVT LNSQAPKLVY
460 470 480 490 500
EEGSLIKRSL RDLYNKEIDE VLVAGEAGYR EARDFMHMLM PSQVRAVKLY
510 520 530 540 550
RDGQPLFSRM GVESQLDAMF SPTVQLRSGG YIVINQTEAL VSIDVNSGRS
560 570 580 590 600
TREHHIEDTA LKTNMEAAEE VARQLRLRDL AGLIVIDFID MDEKRNNRAV
610 620 630 640 650
ERKLSDCLRQ DRARIQVGRI SHFGLLEMSR QRIRASVLES STEPCPHCGG
660 670 680 690 700
SGHVRSVSSV ALQLLRGLEE VLMKGATHNL IVRTRTDVAL YVLNHKRGHL
710 720 730 740 750
RDLETGFRVS LSVLADPTVS GQQSFVIDRG EQVHTLETAK ALLAAQIAAS
760 770 780 790 800
PAPLDEPEDD DGYDFEAEIE TDETVGLAED QGSEAGESES EGRKRRRRRR
810 820 830 840 850
RRRSGEPREA AEAGAVGEDG EQPFVSEGVE QVGEADEGDE AGEDDSDAEA
860 870 880 890 900
RADQGENGER RPRRRGRRGG RRRRGNAEAG ENEAGVVGSI SDELSPTDEA
910 920 930 940 950
EAADAVADFD GGSPSVQPSF EPAAEAEQPH DAPPHDIVPS EPQHTAATAA
960 970 980 990 1000
PDTSDPSAED DSAAKKAARR RSTVREKVSF GASAAASETT AEAAAEVQPE
1010 1020 1030 1040 1050
PTAQAEQPAT AAPAVEAPAA EAAPQAAAQP AEEPSSAAPR RVGWWSRRFG

GGN
Length:1,053
Mass (Da):115,731
Last modified:September 2, 2008 - v1
Checksum:i630391D431EEC8AE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001096 Genomic DNA. Translation: ACF01237.1.
RefSeqiYP_001991712.1. NC_011004.1.

Genome annotation databases

EnsemblBacteriaiACF01237; ACF01237; Rpal_2728.
GeneIDi6410392.
KEGGirpt:Rpal_2728.
PATRICi23309912. VBIRhoPal88240_2766.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001096 Genomic DNA. Translation: ACF01237.1 .
RefSeqi YP_001991712.1. NC_011004.1.

3D structure databases

ProteinModelPortali B3QH48.
SMRi B3QH48. Positions 36-80.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 395960.Rpal_2728.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACF01237 ; ACF01237 ; Rpal_2728 .
GeneIDi 6410392.
KEGGi rpt:Rpal_2728.
PATRICi 23309912. VBIRhoPal88240_2766.

Phylogenomic databases

eggNOGi COG1530.
HOGENOMi HOG000258026.
KOi K08300.
OMAi PFGLLEM.
OrthoDBi EOG6PCPTH.

Enzyme and pathway databases

BioCyci RPAL395960:GHPC-2750-MONOMER.

Family and domain databases

Gene3Di 2.40.50.140. 2 hits.
HAMAPi MF_00970. RNase_E.
InterProi IPR012340. NA-bd_OB-fold.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR019307. RNA-bd_AU-1/RNase_E/G.
IPR028878. RNase_E.
IPR004659. RNase_E/G.
IPR022967. S1_dom.
[Graphical view ]
Pfami PF10150. RNase_E_G. 1 hit.
PF00575. S1. 1 hit.
[Graphical view ]
SMARTi SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
TIGRFAMsi TIGR00757. RNaseEG. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TIE-1Imported.

Entry informationi

Entry nameiB3QH48_RHOPT
AccessioniPrimary (citable) accession number: B3QH48
Entry historyi
Integrated into UniProtKB/TrEMBL: September 2, 2008
Last sequence update: September 2, 2008
Last modified: October 29, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3