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B3QH48

- B3QH48_RHOPT

UniProt

B3QH48 - B3QH48_RHOPT

Protein

Ribonuclease E

Gene

rne

Organism
Rhodopseudomonas palustris (strain TIE-1)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 1 (02 Sep 2008)
      Previous versions | rss
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    Functioni

    Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.UniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.UniRule annotation

    Cofactori

    Binds 1 Mg2+ ion per subunit.UniRule annotation
    Binds 2 Zn2+ ions per homotetramer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi544 – 5441Magnesium; catalyticUniRule annotation
    Metal bindingi587 – 5871Magnesium; catalyticUniRule annotation
    Metal bindingi645 – 6451Zinc; shared with dimeric partnerUniRule annotation
    Metal bindingi648 – 6481Zinc; shared with dimeric partnerUniRule annotation

    GO - Molecular functioni

    1. endoribonuclease activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. ribonuclease E activity Source: InterPro
    4. RNA binding Source: UniProtKB-HAMAP
    5. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. rRNA processing Source: UniProtKB-HAMAP
    3. tRNA processing Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    EndonucleaseUniRule annotation, Hydrolase, Nuclease

    Keywords - Biological processi

    rRNA processingUniRule annotation, tRNA processingUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, Metal-bindingUniRule annotation, RNA-bindingUniRule annotation, ZincUniRule annotation

    Enzyme and pathway databases

    BioCyciRPAL395960:GHPC-2750-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease EUniRule annotation (EC:3.1.26.12UniRule annotation)
    Short name:
    RNase EUniRule annotation
    Gene namesi
    Name:rneUniRule annotation
    Ordered Locus Names:Rpal_2728Imported
    OrganismiRhodopseudomonas palustris (strain TIE-1)Imported
    Taxonomic identifieri395960 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
    ProteomesiUP000001725: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation. Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. cytoplasmic side of plasma membrane Source: UniProtKB-HAMAP

    Keywords - Cellular componenti

    Cell inner membraneUniRule annotation, Cell membrane, CytoplasmUniRule annotation, Membrane

    Interactioni

    Subunit structurei

    Homotetramer formed by a dimer of dimers.UniRule annotation

    Protein-protein interaction databases

    STRINGi395960.Rpal_2728.

    Structurei

    3D structure databases

    ProteinModelPortaliB3QH48.
    SMRiB3QH48. Positions 36-80.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 11172S1 motifUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni645 – 6484Required for zinc-mediated homotetramerization and catalytic activityUniRule annotation

    Sequence similaritiesi

    Belongs to the RNase E/G family. RNase E subfamily.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1530.
    HOGENOMiHOG000258026.
    KOiK08300.
    OMAiPFGLLEM.
    OrthoDBiEOG6PCPTH.

    Family and domain databases

    Gene3Di2.40.50.140. 2 hits.
    HAMAPiMF_00970. RNase_E.
    InterProiIPR012340. NA-bd_OB-fold.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR019307. RNA-bd_AU-1/RNase_E/G.
    IPR022967. RNA-binding_domain_S1.
    IPR028878. RNase_E.
    IPR004659. RNase_E/G.
    [Graphical view]
    PfamiPF10150. RNase_E_G. 1 hit.
    PF00575. S1. 1 hit.
    [Graphical view]
    SMARTiSM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00757. RNaseEG. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B3QH48-1 [UniParc]FASTAAdd to Basket

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    MANKMLIDAT HPEETRVVVV RGNRVEEFDF ETAQRKQLRG NIYLAKVTRV     50
    EPSLQAAFIE YGGNRHGFLA FSEIHPDYYQ IPVADRQALI EADERAHREA 100
    EEENEQRSSR RRSRHRSHRR RNNGERVQSE IVEAATDVAA EQPHEAADHH 150
    EAHEAGAHAE AERLMHELPE GETAEQHDAE YAADHEHTHV EAAPSEPQVS 200
    AAAEPVTIEQ PAAAEAPAPV EAPQAAASHD ETDAHDDDHG EDSANYAEDA 250
    PFPIDEHHEA EDEHEAHDDH VEGEHEVETD SDSDTEAEAE DDEEEDDEEE 300
    AEEDVVESVG GDDVLEEVPE RAFRPRRQYK IQEVIKRRQV MLVQVVKEER 350
    GNKGAALTTY LSLAGRYAVL MPNTARGGGI SRKITSAQDR SRLKEVVQDL 400
    DVPEGMGVIL RTAGASRTKP EIKRDFEYLI RMWETVRDVT LNSQAPKLVY 450
    EEGSLIKRSL RDLYNKEIDE VLVAGEAGYR EARDFMHMLM PSQVRAVKLY 500
    RDGQPLFSRM GVESQLDAMF SPTVQLRSGG YIVINQTEAL VSIDVNSGRS 550
    TREHHIEDTA LKTNMEAAEE VARQLRLRDL AGLIVIDFID MDEKRNNRAV 600
    ERKLSDCLRQ DRARIQVGRI SHFGLLEMSR QRIRASVLES STEPCPHCGG 650
    SGHVRSVSSV ALQLLRGLEE VLMKGATHNL IVRTRTDVAL YVLNHKRGHL 700
    RDLETGFRVS LSVLADPTVS GQQSFVIDRG EQVHTLETAK ALLAAQIAAS 750
    PAPLDEPEDD DGYDFEAEIE TDETVGLAED QGSEAGESES EGRKRRRRRR 800
    RRRSGEPREA AEAGAVGEDG EQPFVSEGVE QVGEADEGDE AGEDDSDAEA 850
    RADQGENGER RPRRRGRRGG RRRRGNAEAG ENEAGVVGSI SDELSPTDEA 900
    EAADAVADFD GGSPSVQPSF EPAAEAEQPH DAPPHDIVPS EPQHTAATAA 950
    PDTSDPSAED DSAAKKAARR RSTVREKVSF GASAAASETT AEAAAEVQPE 1000
    PTAQAEQPAT AAPAVEAPAA EAAPQAAAQP AEEPSSAAPR RVGWWSRRFG 1050
    GGN 1053
    Length:1,053
    Mass (Da):115,731
    Last modified:September 2, 2008 - v1
    Checksum:i630391D431EEC8AE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001096 Genomic DNA. Translation: ACF01237.1.
    RefSeqiWP_012495937.1. NC_011004.1.
    YP_001991712.1. NC_011004.1.

    Genome annotation databases

    EnsemblBacteriaiACF01237; ACF01237; Rpal_2728.
    GeneIDi6410392.
    KEGGirpt:Rpal_2728.
    PATRICi23309912. VBIRhoPal88240_2766.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001096 Genomic DNA. Translation: ACF01237.1 .
    RefSeqi WP_012495937.1. NC_011004.1.
    YP_001991712.1. NC_011004.1.

    3D structure databases

    ProteinModelPortali B3QH48.
    SMRi B3QH48. Positions 36-80.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 395960.Rpal_2728.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACF01237 ; ACF01237 ; Rpal_2728 .
    GeneIDi 6410392.
    KEGGi rpt:Rpal_2728.
    PATRICi 23309912. VBIRhoPal88240_2766.

    Phylogenomic databases

    eggNOGi COG1530.
    HOGENOMi HOG000258026.
    KOi K08300.
    OMAi PFGLLEM.
    OrthoDBi EOG6PCPTH.

    Enzyme and pathway databases

    BioCyci RPAL395960:GHPC-2750-MONOMER.

    Family and domain databases

    Gene3Di 2.40.50.140. 2 hits.
    HAMAPi MF_00970. RNase_E.
    InterProi IPR012340. NA-bd_OB-fold.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR019307. RNA-bd_AU-1/RNase_E/G.
    IPR022967. RNA-binding_domain_S1.
    IPR028878. RNase_E.
    IPR004659. RNase_E/G.
    [Graphical view ]
    Pfami PF10150. RNase_E_G. 1 hit.
    PF00575. S1. 1 hit.
    [Graphical view ]
    SMARTi SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00757. RNaseEG. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TIE-1Imported.

    Entry informationi

    Entry nameiB3QH48_RHOPT
    AccessioniPrimary (citable) accession number: B3QH48
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 2, 2008
    Last sequence update: September 2, 2008
    Last modified: October 1, 2014
    This is version 36 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3