B3QC00 (RPOB_RHOPT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	Rpal_3687

Organism

Rhodopseudomonas palustris (strain TIE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

395960 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	1374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1374	1374	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_1000141728

Sequences

	Sequence		Length	Mass (Da)
	B3QC00 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: 1110C67B5CA5CF48	FASTA	1,374	153,599
	10 20 30 40 50 60 MAQQTFTGRK RVRKFFGHIR EVAEMPNLIE VQKASYDQFL MVAEPPGGRP DEGLQAVFRS 70 80 90 100 110 120 VFPISDFSNA SMLEFVRYEF EPPKYDVDEC RQRGMTYAAP LKVTLRLIVF DIDEETGARS 130 140 150 160 170 180 VKDIKEQDVY MGDIPLMTMN GTFIVNGTER VIVSQMHRSP GVFFDHDKGK THSSGKLLFA 190 200 210 220 230 240 ARIIPYRGSW LDIEFDAKDI VYARIDRRRK LPVTSLMFAL GLDGEEILST FYNKILYKRT 250 260 270 280 290 300 KEGWRVPFDV NRFRGYSTVN DLIDADTGKV VLEAGKKLTV RAARQLQEKG LKALRMSDEE 310 320 330 340 350 360 LVGNYLAEDL VNPKTGEIYA EAGEEITDKT LKMLNEQGYK ELPLLDIDHV NVGPYIRNTL 370 380 390 400 410 420 NADKNMTRED ALFDIYRVMR PGEPPTLDSA QNMFQSLFFD AERYDLSAVG RVKMNMRLDL 430 440 450 460 470 480 DAPDTHRTLR KEDILAVIKT LVGLRDGKGE IDDIDHLGNR RVRSVGELME NQYRIGLLRM 490 500 510 520 530 540 ERAIKERMSS VDIDTVMPQD LINAKPAAAA VREFFGSSQL SQFMDQTNPL SEITHKRRLS 550 560 570 580 590 600 ALGPGGLTRE RAGFEVRDVH PTHYGRICPI ETPEGPNIGL INSLATFARV NKYGFVETPY 610 620 630 640 650 660 RKVKEGRVTD EVVYLSAMEE GRYAVAQANV SLDAKGKFTD DLVVCRAGGT RDVVPMPADQ 670 680 690 700 710 720 VDYMDVSPKQ LVSVAAALIP FLENDDANRA LMGSNMQRQA VPLVRAEAPF VGTGMEGVVA 730 740 750 760 770 780 RDSGAAIAAR RTGIIDQIDA TRIVIRATED LDPTKSGVDI YRLMKYQRSN QSTCINQRPL 790 800 810 820 830 840 VKVGDHVKKG DIIADGPSTD LGELALGRNV LVAFMPWNGY NFEDSILLSE RIVKEDVFTS 850 860 870 880 890 900 IHIEEFEVMA RDTKLGPEEI TRDIPNVSEE ALKNLDEAGI VYIGAEVRAG DILVGKITPK 910 920 930 940 950 960 GESPMTPEEK LLRAIFGEKA SDVRDTSLRV PPGVQGTIVE VRVFNRHGVD KDERALAIER 970 980 990 1000 1010 1020 EEIERLAKDR DDEQAILDRN VYGRLADLLD GRQGIAGPKG FKKDTKITRA VLEEYPKSQW 1030 1040 1050 1060 1070 1080 WLFAAPNDKL MAEIEAMRKQ YDESKKGLEQ RFLDKVEKLQ RGDELPPGVM KMVKVFVAVK 1090 1100 1110 1120 1130 1140 RKIQPGDKMA GRHGNKGVVS KIVPIEDMPF LEDGTHADIV LNPLGVPSRM NVGQILETHL 1150 1160 1170 1180 1190 1200 GWACAGLGKR IGETIDAYYQ SQDLKPLRET LRKIYGEDET IKSLDDGELL ELGRNLSHGV 1210 1220 1230 1240 1250 1260 PIATPVFDGA KEADIEEMLK LAGFDASGQS TVYDGRTGDQ FDRRVTVGYI YMLKLHHLVD 1270 1280 1290 1300 1310 1320 DKIHARSIGP YSLVTQQPLG GKAQFGGQRF GEMEVWALEA YGAAYTLQEM LTVKSDDVAG 1330 1340 1350 1360 1370 RTKVYEAIVR GDDTFEAGIP ESFNVLVKEM RSLGLNVDLH NSKLAAPPPA EAAE

References

[1]

"Complete sequence of Rhodopseudomonas palustris TIE-1." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Emerson D., Newman D.K., Roden E., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TIE-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001096 Genomic DNA. Translation: ACF02187.1.
RefSeq	YP_001992662.1. NC_011004.1.
3D structure databases
ProteinModelPortal	B3QC00.
ModBase	Search...
Protein-protein interaction databases
STRING	B3QC00.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6411363.
GenomeReviews	Gene locus Rpal_3687 in contig CP001096_GR.
KEGG	rpt:Rpal_3687.
PATRIC	23311879. VBIRhoPal88240_3736.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285547.
OMA	QRDLTYA.
ProtClustDB	PRK00405.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_RHOPT

Accession

Primary (citable) accession number: B3QC00

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program