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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Rhodopseudomonas palustris (strain TIE-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:Rpal_2144
OrganismiRhodopseudomonas palustris (strain TIE-1)
Taxonomic identifieri395960 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
Proteomesi
  • UP000001725 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002129891 – 990Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST990

Structurei

3D structure databases

ProteinModelPortaliB3QBR5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 474Adenylyl removaseUniRule annotationAdd BLAST474
Regioni478 – 990Adenylyl transferaseUniRule annotationAdd BLAST513

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000256492.
KOiK00982.
OMAiEFMVQYA.
OrthoDBiPOG092E03AW.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

B3QBR5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIFSAITADL DNTALAARFG AGPRLYDPVL AAERWAGWLV DLAPEQADAI
60 70 80 90 100
AALGNAFPDL QIALQSIAEA SPYLFDLIRG DPVRLLRVLR GAPEQRLAKL
110 120 130 140 150
LEDAETFVAA ASAEDEVMAA LRRLKAEAAL LIALCDIGGI WPVMQVTQAL
160 170 180 190 200
TDLAGRSVQM ALRFLLRLEA GRGRIVPPNP DCPEQGSGLI VLAMGKMGAG
210 220 230 240 250
ELNYSSDIDL IVFYELDAPT LAPDIEPQPF FVRVTQGLSR ILQQRRGDGY
260 270 280 290 300
VFRVDLRLRP DPASTPVALS TVSALDYYER EGRTWERAAM IKARPCAGDL
310 320 330 340 350
VAGDALLSEI APFVWRKHLD FAALSDVHDM KRQMQTYRGQ TEIAVEGHNV
360 370 380 390 400
KVGRGGIREI EFFAQTQQLI AGGRHPELRV RPTLEALEIL AARNWITIQA
410 420 430 440 450
RDELTEAYLF LRKVEHRVQM IADEQTHALP DTVEAIERFS RFLGYDSRDS
460 470 480 490 500
FARDLLGYLE RVQGHYAKLF EGDPTGTAKL PPVDYGAGPE DTRLLDHLLS
510 520 530 540 550
LGYKKPLMIA TTLQQWMTGG YRVLKVETTQ RAFREFVPAL IEELARAEQP
560 570 580 590 600
DDAVNAFDRL LQALHRGGRL ISLLSQNREL LTLVALVLGA APRLGDMLAR
610 620 630 640 650
QPQILDGLID PRFFGAMPDQ AELSARLAVT LADAGSYEEF LDRLRLFGQE
660 670 680 690 700
SLFLIGTRIL SGTVPTQQAA VAFADVAEGI VGTVHGLVSE QFASTYGRVK
710 720 730 740 750
GQQTAILAMG KLGSREMTAS SDLDLILIYD FDDDQPDSDG ERSLHGAQYF
760 770 780 790 800
ARFTQRLISA FTTRTNYGVL YDVDMRLRPS GRAGPVASRL DAFAAYQEQE
810 820 830 840 850
AWTWEHLALT RARVISAPPE FRSRIEQVIR AVLTRPRDAA IIANDVAEMR
860 870 880 890 900
RAIAQEKGED DVWDLKYAAG GMVDIDFIAQ YLQLVHAHEA PDILHVNTLS
910 920 930 940 950
ALDNATRLGL LAQADAEVLR PAARLYQNLT QILRLCVSEK FNPDTAGDDL
960 970 980 990
LRVMVRAGDA PDFSSLQARV KETQSDVRAI FNRLIGGEDA
Length:990
Mass (Da):109,385
Last modified:September 2, 2008 - v1
Checksum:iACB35468FDD1DEFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001096 Genomic DNA. Translation: ACF00665.1.
RefSeqiWP_012495466.1. NC_011004.1.

Genome annotation databases

EnsemblBacteriaiACF00665; ACF00665; Rpal_2144.
KEGGirpt:Rpal_2144.
PATRICi23308737. VBIRhoPal88240_2183.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001096 Genomic DNA. Translation: ACF00665.1.
RefSeqiWP_012495466.1. NC_011004.1.

3D structure databases

ProteinModelPortaliB3QBR5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACF00665; ACF00665; Rpal_2144.
KEGGirpt:Rpal_2144.
PATRICi23308737. VBIRhoPal88240_2183.

Phylogenomic databases

HOGENOMiHOG000256492.
KOiK00982.
OMAiEFMVQYA.
OrthoDBiPOG092E03AW.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNE_RHOPT
AccessioniPrimary (citable) accession number: B3QBR5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 2, 2008
Last modified: February 15, 2017
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.