B3Q9X6 (PLSY_RHOPT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase

Alternative name(s):
Acyl-PO4 G3P acyltransferase
Acyl-phosphate--glycerol-3-phosphate acyltransferase
G3P acyltransferase
Short name=GPAT
EC=2.3.1.n3
Lysophosphatidic acid synthase
Short name=LPA synthase

Gene names

Name:	plsY
Ordered Locus Names:	Rpal_3529

Organism

Rhodopseudomonas palustris (strain TIE-1) [Complete proteome] [HAMAP]

Taxonomic identifier

395960 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas › Rhodopseudomonas palustris

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO4) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP By similarity. HAMAP-Rule MF_01043
Catalytic activity	Acyl-phosphate + sn-glycerol 3-phosphate = 1-acyl-sn-glycerol 3-phosphate + phosphate. HAMAP-Rule MF_01043
Pathway	Lipid metabolism; phospholipid metabolism. HAMAP-Rule MF_01043
Subunit structure	Probably interacts with PlsX By similarity. HAMAP-Rule MF_01043
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_01043.
Sequence similarities	Belongs to the PlsY family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acyl-phosphate glycerol-3-phosphate acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	Glycerol-3-phosphate acyltransferase HAMAP-Rule MF_01043		PRO_1000136113
Regions
	Transmembrane	5 – 25	21	Helical; Potential
	Transmembrane	55 – 75	21	Helical; Potential
	Transmembrane	84 – 104	21	Helical; Potential
	Transmembrane	118 – 138	21	Helical; Potential
	Transmembrane	159 – 179	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	B3Q9X6 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: F8592B72123C8B7E	FASTA	203	21,526
	10 20 30 40 50 60 MMIGIYIAAL VIGYLFGSIP FGLILTKIAG TQDLRSIGSG NIGATNVLRT GRKGLAAATL 70 80 90 100 110 120 LLDALKGTAA VIVAAYLASG TDAIAANAAM LAALGAFLGH LFPVWLKFKG GKGVAVYIGV 130 140 150 160 170 180 LIGLFWPAAV VFCIMWLATA FTSRYSSLSA LVASFVTPIF LWWFGHDSLA SLFAVLTLLL 190 200 FWMHRENIKR LQAGTESKIG QKK

References

[1]

"Complete sequence of Rhodopseudomonas palustris TIE-1." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Emerson D., Newman D.K., Roden E., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TIE-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001096 Genomic DNA. Translation: ACF02029.1.
RefSeq	YP_001992504.1. NC_011004.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	395960.Rpal_3529.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACF02029; ACF02029; Rpal_3529.
GeneID	6411203.
KEGG	rpt:Rpal_3529.
PATRIC	23311558. VBIRhoPal88240_3578.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0344.
HOGENOM	HOG000283806.
KO	K08591.
OMA	WRHRGNL.
ProtClustDB	PRK00220.
Enzyme and pathway databases
BioCyc	RPAL395960:GHPC-3562-MONOMER.
UniPathway	UPA00085.
Family and domain databases
HAMAP	MF_01043. PlsY.
InterPro	IPR003811. G3P_acylTferase. [Graphical view]
Pfam	PF02660. G3P_acyltransf. 1 hit. [Graphical view]
TIGRFAMs	TIGR00023. TIGR00023. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSY_RHOPT

Accession

Primary (citable) accession number: B3Q9X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	May 29, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program