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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Rhodopseudomonas palustris (strain TIE-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (Rpal_0028)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK), Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65ShikimateUniRule annotation1
Active sitei69Proton acceptorUniRule annotation1
Binding sitei80NADPUniRule annotation1
Binding sitei89ShikimateUniRule annotation1
Binding sitei104ShikimateUniRule annotation1
Binding sitei218NADP; via carbonyl oxygenUniRule annotation1
Binding sitei220ShikimateUniRule annotation1
Binding sitei241NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi129 – 133NADPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciRPAL395960:G1GC6-4806-MONOMER
UniPathwayiUPA00053; UER00087

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:Rpal_4803
OrganismiRhodopseudomonas palustris (strain TIE-1)
Taxonomic identifieri395960 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
Proteomesi
  • UP000001725 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001001331 – 278Shikimate dehydrogenase (NADP(+))Add BLAST278

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB3Q7T8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni18 – 20Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000237875
KOiK00014
OMAiIPHKERA
OrthoDBiPOG091H02DY

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit

Sequencei

Sequence statusi: Complete.

B3Q7T8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATKRAACL IGWPAAHSRS PLIHHYWLRR LGIEGGYSIE AVPPEGFAEF
60 70 80 90 100
VLHLKTHGYV GANVTIPHKE RALQLTEPDE RARAVGAANT LYYDGDLLRS
110 120 130 140 150
TNTDIEGFIG NLDASAPGWD RSPHAVVLGA GGSSRAVVFG LLERGVQRIA
160 170 180 190 200
LANRSIERAQ ALRDLFGERV VPIAWSDIPA ALPGAGLLVN TTSLGMKGQP
210 220 230 240 250
PLQIDLSALP ADAVVSDLVY VPLETDLLAA AKARGLRTAD GLGMLLHQAV
260 270
RGFDLWFGAR PHVTAELRAL VEADLAPK
Length:278
Mass (Da):29,704
Last modified:September 2, 2008 - v1
Checksum:i2A88A958B666C31C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001096 Genomic DNA Translation: ACF03293.1
RefSeqiWP_012497532.1, NC_011004.1

Genome annotation databases

EnsemblBacteriaiACF03293; ACF03293; Rpal_4803
KEGGirpt:Rpal_4803

Similar proteinsi

Entry informationi

Entry nameiAROE_RHOPT
AccessioniPrimary (citable) accession number: B3Q7T8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 2, 2008
Last modified: April 25, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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