B3Q5R5 (B3Q5R5_RHIE6) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB 2 HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2 HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme 2 HAMAP MF_00685
Glycogen branching enzyme 2 HAMAP MF_00685

Gene names

Name:	glgBc EMBL ACE94546.1
Synonyms:	glgB2 HAMAP MF_00685
Ordered Locus Names:	RHECIAT_PC0000468

Encoded on

Plasmid pC EMBL ACE94546.1

Organism

Rhizobium etli (strain CIAT 652) [Complete proteome] [HAMAP] EMBL ACE94546.1

Taxonomic identifier

491916 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	736 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685 SAAS SAAS006407
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 SAAS SAAS006407 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 EMBL ACE94546.1 Transferase
Technical term	Complete proteome Plasmid EMBL ACE94546.1
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	415	1	Nucleophile By similarity HAMAP MF_00685
	Active site	468	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	B3Q5R5 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: C50872205027B55F	FASTA	736	81,976
	10 20 30 40 50 60 MNVERSELLS GIGHDALWAL IDGRHGDPFS ILGPHEAGGM TVVRVYLPGA DAVDLIDADS 70 80 90 100 110 120 GRVVTPFSIA HPSGLFAAAT ASRMSYRLRI QWPDGEQVTE DPYSFGLLLG ELDLHLISEG 130 140 150 160 170 180 THYSLSRTLG AVAMSIDGIA GVRFAVWAPN ARRVSVVGDF NAWDGRRNPM RLRQSAGVWE 190 200 210 220 230 240 LFVPRLAPGE RYKFEIVDAH GNCLPQKADP VARASEAAPS TASIVASSTP FRWTDDGWMK 250 260 270 280 290 300 GRDRQARLDG AISVYEVHAG SWLRDRQDGN SYLDWAELSQ RLVPYVRDMG FTHIELLPIM 310 320 330 340 350 360 EHPFGGSWGY QPLGMFAPTG RYGTPEDFAY FVDRCHGAGI GVILDWVPAH FPTDVWGLAR 370 380 390 400 410 420 FDGSALYEHE DPREGFHRDW NTLIYNLGRS EVKGFLIASA LEWLERYHVD GLRVDAVASM 430 440 450 460 470 480 LYRDYSRNEG EWIPNRYGGR ENLEAVEFFK HLNSIVHERC PHAITIAEES TAWPGVTKPP 490 500 510 520 530 540 EEGGLGFDIK WNMGWMHDSL SYIEKDPVYR SYHHGTMTFG MIYAYSERFI LPISHDEVVY 550 560 570 580 590 600 GKGSLLAKMP GDEWQKFANL RSYLAFMWGH PGKKLLFMGS EVAQPGEWNH DASVSWDVLD 610 620 630 640 650 660 RPAHVGIQRL VRDLNAFYGD EPALQFGDFH PEGFEWATAD DAVNSVLGML RFASDRSSSV 670 680 690 700 710 720 LILSNFTPVP RYGYRIGVPQ DGVWIERVTT DAREYGGSGL VNGAVSSESV PAHGRPFSLA 730 LTLPPLATVF LKGPSP

References

[1]

"Genome diversity and DNA divergence of Rhizobium etli." Gonzalez V., Acosta J.L., Santamaria R.I., Bustos P., Hernandez-Gonzalez I.L., Fernandez J.L., Diaz R., Flores M., Mora J., Palacios R., Davila G. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: CIAT 652 EMBL ACE94546.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001077 Genomic DNA. Translation: ACE94546.1.
RefSeq	YP_001985096.1. NC_010997.1.
3D structure databases
ProteinModelPortal	B3Q5R5.
ModBase	Search...
Protein-protein interaction databases
STRING	B3Q5R5.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6403355.
GenomeReviews	Gene locus RHECIAT_PC0000468 in contig CP001077_GR.
KEGG	rec:RHECIAT_PC0000468.
PATRIC	23104028. VBIRhiEtl120572_5224.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287139.
OMA	RVYHQNG.
ProtClustDB	PRK05402.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B3Q5R5_RHIE6

Accession

Primary (citable) accession number: B3Q5R5

Entry history

Integrated into UniProtKB/TrEMBL:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)