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Protein

Catalase-peroxidase

Gene

katG

Organism
Rhizobium etli (strain CIAT 652)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.UniRule annotation

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bUniRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei93Transition state stabilizerUniRule annotation1
Active sitei97Proton acceptorUniRule annotation1
Metal bindingi259Iron (heme axial ligand)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidaseUniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CPUniRule annotation
Alternative name(s):
Peroxidase/catalaseUniRule annotation
Gene namesi
Name:katGUniRule annotation
Ordered Locus Names:RHECIAT_PC0000007
Encoded oniPlasmid pC0 Publication
OrganismiRhizobium etli (strain CIAT 652)
Taxonomic identifieri491916 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
Proteomesi
  • UP000008817 Componenti: Plasmid pC

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003548831 – 728Catalase-peroxidaseAdd BLAST728

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki96 ↔ 218Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-244)UniRule annotation
Cross-linki218 ↔ 244Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-96)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Proteomic databases

PRIDEiB3Q4F9.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218110.
KOiK03782.
OrthoDBiPOG091H05R1.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3Q4F9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNPTDTAGK CPVAHGNKPR GPSNRDWWPN QLNVQILHHN SGRADPMGKD
60 70 80 90 100
FDYAEEFKKL DLDALKKDLT ALMTDSQDWW PADFGHYGGL FIRMAWHSAG
110 120 130 140 150
TYRITDGRGG AGQGQQRFAP LNSWPDNANL DKARRLLWPI KQKYGNRISW
160 170 180 190 200
ADLLILTGNV ALESMGFKTF GFAGGRADVW EPEELYWGPE GTWLGDERYS
210 220 230 240 250
GERQLAEPLG AVQMGLIYVN PEGPGGNPDP LASARDIRET FARMAMNDEE
260 270 280 290 300
TVALIAGGHT FGKTHGAGDP SFIGAEPEGG AIEDQGLGWK SSFGSGVGKD
310 320 330 340 350
AITAGLEVTW SQTPTKWSNY FFENLFAYEW ELTKSPAGAH QWQAKNAEAS
360 370 380 390 400
IPDAYEAGKK HLPTMLTSDL ALRFDPIYEK ISRRFLENPD QFADAFARAW
410 420 430 440 450
FKLTHRDMGP KVRYLGPEVP AEDLIWQDVI PAVDHPLVGD KDIAELKAKV
460 470 480 490 500
LATGLSVQEL VSTAWASAST FRGSDKRGGA NGARIRLAPQ KDWDANQPAQ
510 520 530 540 550
LAKVLGVLEG LQKDFNAAQT GGKKISLADM IVLAGAAGVE KAAAAGGTTV
560 570 580 590 600
SVPFTPGRMD ASEAQTDAHS FAALEPRIDG FRNYVNDKRL QFMKPEEALV
610 620 630 640 650
DRAQLLTLTG PEMTVLVGGL RVLKAGQPEH GVFTARPETL TNDFFVNLLD
660 670 680 690 700
MATQWVPAAG KDGVYEGRDR KTGAAKWTGT RVDLIFGSHS QLRAFAEVYG
710 720
QADAKEKFVK DFVAAWNKVM NADRFDLV
Length:728
Mass (Da):79,730
Last modified:November 25, 2008 - v2
Checksum:i091272AB6A487A44
GO

Sequence cautioni

The sequence ACE94090 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001077 Genomic DNA. Translation: ACE94090.1. Different initiation.
RefSeqiWP_041684052.1. NC_010997.1.

Genome annotation databases

EnsemblBacteriaiACE94090; ACE94090; RHECIAT_PC0000007.
KEGGirec:RHECIAT_PC0000007.
PATRICi23103068. VBIRhiEtl120572_4744.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001077 Genomic DNA. Translation: ACE94090.1. Different initiation.
RefSeqiWP_041684052.1. NC_010997.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiB3Q4F9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACE94090; ACE94090; RHECIAT_PC0000007.
KEGGirec:RHECIAT_PC0000007.
PATRICi23103068. VBIRhiEtl120572_4744.

Phylogenomic databases

HOGENOMiHOG000218110.
KOiK03782.
OrthoDBiPOG091H05R1.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKATG_RHIE6
AccessioniPrimary (citable) accession number: B3Q4F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 25, 2008
Last modified: November 2, 2016
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.