B3PZ69 (DUT_RHIE6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	RHECIAT_CH0000369

Organism

Rhizobium etli (strain CIAT 652) [Complete proteome] [HAMAP]

Taxonomic identifier

491916 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium › Rhizobium etli

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP-Rule MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP-Rule MF_00116
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP-Rule MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00116		PRO_1000094982
Regions
	Region	76 – 78	3	Substrate binding By similarity
	Region	93 – 95	3	Substrate binding By similarity
Sites
	Binding site	89	1	Substrate By similarity
	Binding site	103	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3PZ69 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: 0A6C6DA475EC2461	FASTA	156	16,383
	10 20 30 40 50 60 MTIHHETRPT LNLIRLANGE GLELPAYESQ GAAGMDLRAA VDGDAPLTLP PGKRALVPTG 70 80 90 100 110 120 FIFEIPEGFE GQVRPRSGLA FKHGITCLNS PGTVDSDYRG EVKVLLANLG EEAFVIERGM 130 140 150 RIAQMVIAPV TQARVAEITA ASETARGAGG FGSTGV

References

[1]

"Genome diversity and DNA divergence of Rhizobium etli." Gonzalez V., Acosta J.L., Santamaria R.I., Bustos P., Hernandez-Gonzalez I.L., Fernandez J.L., Diaz R., Flores M., Mora J., Palacios R., Davila G. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CIAT 652.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001074 Genomic DNA. Translation: ACE89363.1.
RefSeq	YP_001976541.1. NC_010994.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	491916.RHECIAT_CH0000369.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACE89363; ACE89363; RHECIAT_CH0000369.
GeneID	6398243.
KEGG	rec:RHECIAT_CH0000369.
PATRIC	23094206. VBIRhiEtl120572_0370.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HOG000028966.
KO	K01520.
OMA	ILPRSGM.
ProtClustDB	PRK00601.
Enzyme and pathway databases
BioCyc	RETL491916:GH4T-2122-MONOMER.
UniPathway	UPA00610; UER00666.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact.
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_RHIE6

Accession

Primary (citable) accession number: B3PZ69

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 2, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program