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B3PXS8 (B3PXS8_RHIE6) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Valine--tRNA ligase HAMAP-Rule MF_02004
EC=6.1.1.9 HAMAP-Rule MF_02004
Alternative name(s):
Valyl-tRNA synthetase HAMAP-Rule MF_02004
Gene names
Name:valS HAMAP-Rule MF_02004 EMBL ACE90864.1
Ordered Locus Names:RHECIAT_CH0001897 EMBL ACE90864.1
OrganismRhizobium etli (strain CIAT 652) [Complete proteome] [HAMAP] EMBL ACE90864.1
Taxonomic identifier491916 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium ›

Protein attributes

Sequence length947 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity. HAMAP-Rule MF_02004 SAAS SAAS019499

Catalytic activity

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). HAMAP-Rule MF_02004 SAAS SAAS019499

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02004 SAAS SAAS019499

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02004 SAAS SAAS019499.

Domain

The C-terminal coiled-coil domain is crucial for aminoacylation activity By similarity. HAMAP-Rule MF_02004

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity. HAMAP-Rule MF_02004

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. HAMAP-Rule MF_02004

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Coiled coil874 – 94673 By similarity HAMAP-Rule MF_02004
Motif45 – 5511"HIGH" region By similarity HAMAP-Rule MF_02004
Motif591 – 5955"KMSKS" region By similarity HAMAP-Rule MF_02004

Sites

Binding site5941ATP By similarity HAMAP-Rule MF_02004

Sequences

Sequence LengthMass (Da)Tools
B3PXS8 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: 56770677811022D8

FASTA947106,893
        10         20         30         40         50         60 
MLDKTYDSAA VEPKIAAKWD EADAFRAGAN AKPGAETFTI VIPPPNVTGS LHMGHALNNT 

        70         80         90        100        110        120 
LQDILVRFER MRGKDVLWQP GMDHAGIATQ MVVERKLMEQ QLPGRREMGR EAFIDKIWEW 

       130        140        150        160        170        180 
KAESGGLIFN QLKRLGASCD WSRERFTMDE GLSQAVLEVF VTLYKEGLIY KDKRLVNWDP 

       190        200        210        220        230        240 
KLLTAISDME VEQHEVKGHL WHLRYPLEPG VTYQHPIAFD EEGKPTEFET RDYIVVATTR 

       250        260        270        280        290        300 
PETMLGDTGV AVNPEDERYK PIVGKHVILP IVGRKIPIVT DSYADPTAGT GAVKITPAHD 

       310        320        330        340        350        360 
FNDFDVGKRT KLRAINVMNV DGTIAIKDNE DFLEGLDNPA ALHGAWDRLE GQDRFYARKI 

       370        380        390        400        410        420 
IVEIFEEAGL LDKIEPHKHM VPHGDRGGVP IEPRLTEQWF VDNKTLGQPA LDSVREGRTR 

       430        440        450        460        470        480 
FIPRNWENTY FNWLENIEPW CISRQLWWGH QIPAWYGPDG QVFVEKTEEE ALQAAIQHYL 

       490        500        510        520        530        540 
SHEGPMKAYV EDLLENFKPG EILTRDEDVL DTWFSSALWP FSTLGWPDET PELARYYPTN 

       550        560        570        580        590        600 
VLVTGFDIIP FWVVRMMQMG LHFMKDDNGD PVEPFHTVYI HALVRDKNGQ KMSKSKGNVI 

       610        620        630        640        650        660 
DPLELIDEYG ADALRFTLAI MAAQGRDVKL DPARIAGYRN FGTKLWNATR FAEMNGAKSD 

       670        680        690        700        710        720 
PHFVPEAAEL TINRWILTEL ARTERDVTEA LEAFRFNDAA GALYRFVWNE VCDWYLELLK 

       730        740        750        760        770        780 
PVFNGEDAGA KAEAQACSAY ILEEIYKLLH PFMPFMTEEL WAHTAGEGKE RETLVCHAEW 

       790        800        810        820        830        840 
PSPSYADDAA ADEINWLIDL VSGIRSVRAE MNVPPSATAP LVVVKANNLT RERLFRHDAA 

       850        860        870        880        890        900 
IKRLARVEAI SIADNAPKGA AQIVVAEATV CLPLGNLIDL SAEKARLEKA IAKMEGEMAR 

       910        920        930        940 
IDGKLSNEKF VANANPEVVE AERDRLEELK GQMASLKTAL SRVSEAG

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References

[1]"Genome diversity and DNA divergence of Rhizobium etli."
Gonzalez V., Acosta J.L., Santamaria R.I., Bustos P., Hernandez-Gonzalez I.L., Fernandez J.L., Diaz R., Flores M., Mora J., Palacios R., Davila G.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CIAT 652 EMBL ACE90864.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001074 Genomic DNA. Translation: ACE90864.1.
RefSeqYP_001978042.1. NC_010994.1.

3D structure databases

ProteinModelPortalB3PXS8.
ModBaseSearch...

Protein-protein interaction databases

STRING491916.RHECIAT_CH0001897.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACE90864; ACE90864; RHECIAT_CH0001897.
GeneID6399984.
KEGGrec:RHECIAT_CH0001897.
PATRIC23097264. VBIRhiEtl120572_1876.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0525.
HOGENOMHOG000020093.
KOK01873.
OMAIMDALIR.
ProtClustDBPRK05729.

Enzyme and pathway databases

BioCycRETL491916:GH4T-3649-MONOMER.

Family and domain databases

Gene3D1.10.287.380. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02004. Val_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERPTHR11946:SF5. PTHR11946:SF5. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view]
PRINTSPR00986. TRNASYNTHVAL.
SUPFAMSSF46589. tRNA_binding_arm. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00422. valS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB3PXS8_RHIE6
AccessionPrimary (citable) accession number: B3PXS8
Entry history
Integrated into UniProtKB/TrEMBL: September 2, 2008
Last sequence update: September 2, 2008
Last modified: May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info