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Protein

Homogentisate 1,2-dioxygenase

Gene

hmgA

Organism
Rhizobium etli (strain CIAT 652)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the catabolism of homogentisate (2,5-dihydroxyphenylacetate or 2,5-OH-PhAc), a central intermediate in the degradation of phenylalanine and tyrosine. Catalyzes the oxidative ring cleavage of the aromatic ring of homogentisate to yield maleylacetoacetate.UniRule annotation

Catalytic activityi

Homogentisate + O2 = 4-maleylacetoacetate.UniRule annotation

Cofactori

Fe cationUniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei306 – 3061Proton acceptorUniRule annotation
Metal bindingi349 – 3491IronUniRule annotation
Metal bindingi355 – 3551IronUniRule annotation
Binding sitei364 – 3641homogentisateUniRule annotation
Metal bindingi385 – 3851IronUniRule annotation
Binding sitei385 – 3851homogentisateUniRule annotation

GO - Molecular functioni

  1. homogentisate 1,2-dioxygenase activity Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-phenylalanine catabolic process Source: UniProtKB-HAMAP
  2. tyrosine catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Phenylalanine catabolism, Tyrosine catabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciRETL491916:GH4T-1826-MONOMER.
UniPathwayiUPA00139; UER00339.

Names & Taxonomyi

Protein namesi
Recommended name:
Homogentisate 1,2-dioxygenaseUniRule annotation (EC:1.13.11.5UniRule annotation)
Short name:
HGDOUniRule annotation
Alternative name(s):
Homogentisate oxygenaseUniRule annotation
Homogentisic acid oxidaseUniRule annotation
HomogentisicaseUniRule annotation
Gene namesi
Name:hmgAUniRule annotation
Ordered Locus Names:RHECIAT_CH0001827
OrganismiRhizobium etli (strain CIAT 652)
Taxonomic identifieri491916 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
ProteomesiUP000008817: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 453453Homogentisate 1,2-dioxygenasePRO_1000119848Add
BLAST

Interactioni

Subunit structurei

Hexamer; dimer of trimers.UniRule annotation

Protein-protein interaction databases

STRINGi491916.RHECIAT_CH0001827.

Family & Domainsi

Sequence similaritiesi

Belongs to the homogentisate dioxygenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3508.
HOGENOMiHOG000139824.
KOiK00451.
OMAiRGYLCEN.
OrthoDBiEOG6D5FZK.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00334. Homogentis_dioxygen.
InterProiIPR005708. Homogentis_dOase.
IPR022950. Homogentis_dOase_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR11056. PTHR11056. 1 hit.
PfamiPF04209. HgmA. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01015. hmgA. 1 hit.

Sequencei

Sequence statusi: Complete.

B3PWZ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQTSIQASS GDAAAADQLK YMPGFGNDFE TESLPGALPQ GQNSPQKCNY
60 70 80 90 100
GLYAEQLSGS PFTAPRGTNE RSWLYRIRPS VRHTRRFSNA SYPLWKTAPC
110 120 130 140 150
LDEHSLPLGQ LRWDPIPPPE ERLNFLEGVR TITTAGDATT QVGMSAHAYV
160 170 180 190 200
FNEDMVDDYF FNADGELLIV PQLGALRVFT EMGIMDVEPS EICLVPRGMM
210 220 230 240 250
FKILTSGKQT AWRGYICENY GAKFTLPERG PIGANCLANP RDFKTPVAAY
260 270 280 290 300
EDKEKPCRVH VKWCGKFYVT EIGHSPLDVV AWHGNYAPFK YDLRTFSPVG
310 320 330 340 350
AILFDHPDPS IFSVLTAPTE DAGTANVDFV IFPPRWLVAE HTFRPPWYHR
360 370 380 390 400
NIMSEFMGLI HGQYDAKEEG FVPGGMSLHN MMLPHGPDAL AFEKAANAEL
410 420 430 440 450
KPVKLDHTMA FMFETRYPQQ LTKYAAELET LQDDYLECWD GLERKFDGNP

GIK
Length:453
Mass (Da):50,885
Last modified:September 2, 2008 - v1
Checksum:i1FAC7603E7244E21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001074 Genomic DNA. Translation: ACE90797.1.
RefSeqiYP_001977975.1. NC_010994.1.

Genome annotation databases

EnsemblBacteriaiACE90797; ACE90797; RHECIAT_CH0001827.
GeneIDi6399914.
KEGGirec:RHECIAT_CH0001827.
PATRICi23097126. VBIRhiEtl120572_1810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001074 Genomic DNA. Translation: ACE90797.1.
RefSeqiYP_001977975.1. NC_010994.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi491916.RHECIAT_CH0001827.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACE90797; ACE90797; RHECIAT_CH0001827.
GeneIDi6399914.
KEGGirec:RHECIAT_CH0001827.
PATRICi23097126. VBIRhiEtl120572_1810.

Phylogenomic databases

eggNOGiCOG3508.
HOGENOMiHOG000139824.
KOiK00451.
OMAiRGYLCEN.
OrthoDBiEOG6D5FZK.

Enzyme and pathway databases

UniPathwayiUPA00139; UER00339.
BioCyciRETL491916:GH4T-1826-MONOMER.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00334. Homogentis_dioxygen.
InterProiIPR005708. Homogentis_dOase.
IPR022950. Homogentis_dOase_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR11056. PTHR11056. 1 hit.
PfamiPF04209. HgmA. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01015. hmgA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome diversity and DNA divergence of Rhizobium etli."
    Gonzalez V., Acosta J.L., Santamaria R.I., Bustos P., Hernandez-Gonzalez I.L., Fernandez J.L., Diaz R., Flores M., Mora J., Palacios R., Davila G.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CIAT 652.

Entry informationi

Entry nameiHGD_RHIE6
AccessioniPrimary (citable) accession number: B3PWZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 2, 2008
Last modified: March 4, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.