B3PWJ0 (MASZ_RHIE6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	RHECIAT_CH0000055

Organism

Rhizobium etli (strain CIAT 652) [Complete proteome] [HAMAP]

Taxonomic identifier

491916 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity. HAMAP MF_00641
Subcellular location	Cytoplasm By similarity HAMAP MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 723	723	Malate synthase G HAMAP MF_00641		PRO_1000130895
Sites
	Active site	338	1	Proton acceptor By similarity
	Active site	629	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3PWJ0 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: DD5FC50622E412E6	FASTA	723	79,766
	10 20 30 40 50 60 MSRIDKNGLA VEAVLHDFLV QEVLPGLAID ADKFFADFSA IVHDLAPINR ALLKKRDELQ 70 80 90 100 110 120 LKIDDWYRQH GAPTDMDDYQ SFLRAIGYLL PEGSDFQVST QNVDPEIASI AGPQLVVPVM 130 140 150 160 170 180 NARYALNAAN ARWGSLYDAL YGTDAIPEGD GAEKGRGYNP KRGEKVIAWV RDFLDTSVPL 190 200 210 220 230 240 QDSRWKDVGS FAVRDGALII RSTDGEQAML RDGGHFAGYR GDAATPTHIL LKNNGIHIEI 250 260 270 280 290 300 VIDAATAIGK TDPAHISDVW LESAITTIMD CEDSIAAVDA EDKVVVYRNW LGLMKGDLQE 310 320 330 340 350 360 EVAKGGASFI RKLNPDLDYT GPDGTTFELH RRSLMLVRNV GHLMTNPAIL DRDGNEVPEG 370 380 390 400 410 420 IMDAVITGLI ALYDIGPAGR KKNSRTGSMY VVKPKMHGPE EVAFAVEVFS RVEDALGLAR 430 440 450 460 470 480 NTIKMGIMDE ERRTTVNLKE CIRAARERVV FINTGFLDRT GDEIHTSMEA GPMIRKGDMR 490 500 510 520 530 540 QAAWISAYEN WNVDIGLECG LAGHAQIGKG MWAMPDLMAA MLEQKIAHPK AGANTAWVPS 550 560 570 580 590 600 PTAATLHATH YHRVNVAKVQ QGLKDRARAK LSDILSVPVA VRPNWSQEEI QRELDNNAQG 610 620 630 640 650 660 ILGYVVRWVD QGVGCSKVPD INNIGLMEDR ATLRISAQHM ANWLHHKVVS EAQIVETMKR 670 680 690 700 710 720 MAAVVDRQNE QDPVYQPISG NFDGSIAFQA ALDLVLKGRE QPNGYTEPVL HRRRLELKAK QAA

References

[1]

"Genome diversity and DNA divergence of Rhizobium etli." Gonzalez V., Acosta J.L., Santamaria R.I., Bustos P., Hernandez-Gonzalez I.L., Fernandez J.L., Diaz R., Flores M., Mora J., Palacios R., Davila G. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CIAT 652.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001074 Genomic DNA. Translation: ACE89058.1.
RefSeq	YP_001976236.1. NC_010994.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3PWJ0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6398354.
GenomeReviews	Gene locus glcB in contig CP001074_GR.
KEGG	rec:RHECIAT_CH0000055.
PATRIC	23093564. VBIRhiEtl120572_0058.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG350005.
OMA	PKMHGPD.
ProtClustDB	PRK02999.
Family and domain databases
HAMAP	MF_00641. Malate_synth_G. [Tree]
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
Gene3D	G3DSA:2.170.170.11. Malate_synth_G_beta_sub_dom. 2 hits.
KO	K01638.
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. Malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_RHIE6

Accession

Primary (citable) accession number: B3PWJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program