B3PVU3 (B3PVU3_RHIE6) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA gyrase subunit B RuleBase RU003363 HAMAP MF_01898
EC=5.99.1.3 RuleBase RU003363 HAMAP MF_01898

Gene names

Name:	gyrB1 EMBL ACE89016.1
Synonyms:	gyrB HAMAP MF_01898
Ordered Locus Names:	RHECIAT_CH0000013

Organism

Rhizobium etli (strain CIAT 652) [Complete proteome] [HAMAP]

Taxonomic identifier

491916 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	811 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. RuleBase RU003363 HAMAP MF_01898
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. RuleBase RU003363 SAAS SAAS002288 HAMAP MF_01898
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer By similarity. HAMAP MF_01898
Subcellular location	Cytoplasm By similarity HAMAP MF_01898.
Sequence similarities	Belongs to the type II topoisomerase family. HAMAP MF_01898 RuleBase RU000380 Contains 1 Toprim domain. SAAS SAAS002288 HAMAP MF_01898

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_01898
Ligand	ATP-binding SAAS SAAS002288 HAMAP MF_01898 RuleBase RU000380 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS002288 HAMAP MF_01898 RuleBase RU000380
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B3PVU3 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: 722A5E9C41777F93	FASTA	811	88,743
	10 20 30 40 50 60 MSDTSATENG VSTEYGADSI KVLKGLDAVR KRPGMYIGDT DDGSGLHHMV YEVVDNAIDE 70 80 90 100 110 120 ALAGHADIVT VSLNPDGSVT VTDNGRGIPT DIHSGEGVSA AEVIMTQLHA GGKFDQNSYK 130 140 150 160 170 180 VSGGLHGVGV SVVNALSVWL KLKIRRHGKI HEMSFTHGVA DAPLKVTGDA PDATGTEVSF 190 200 210 220 230 240 MPSSETFTMT EFDYGTLEHR LRELAFLNSG VRILLSDKRH SDIKQEEMRY DGGLEAFVAY 250 260 270 280 290 300 LDRAKKPLVD KPVAIRGEKD GITVEVAMWW NDSYHENVLC FTNNIPQRDG GTHMAGFRAA 310 320 330 340 350 360 LTRQVVSYAD NSGITKKEKV TLQGEDCREG LTAVLSVKVP DPKFSSQTKD KLVSSEVRPV 370 380 390 400 410 420 VESLVNEALS TWFEEHPSEA KILVGKVVEA AAAREAARKA RELTRRKGAL DIASLPGKLA 430 440 450 460 470 480 DCSERDPAKS EVFLVEGDSA GGSAKQGRSR ENQAILPLRG KILNVERARF DKMLSSQEIG 490 500 510 520 530 540 TLITALGTGI GKDEFNAEKL RYHKIIIMTD ADVDGAHIRT LLLTFFFRQM PELIERGHLY 550 560 570 580 590 600 IAQPPLYKVA RGKSVQYLKD EKALEEYLIG QGLEDAALRL GSGEVRTGQD LREVILDALR 610 620 630 640 650 660 MRALLDNLHS RYNRAVVEQA AIAGALNAEL VSDAARARAL ASEVASRLDI IAEETERGWQ 670 680 690 700 710 720 GDLTSDGGLR LERMVRGVKE IVVLDMALIG SSDARHIDQL TSRLKEIYQT PPSLHRREGD 730 740 750 760 770 780 IEISGPRALL DAIFASGRKG LTMQRYKGLG EMNAEQLWET TLDPNVRSLL QVKVADATDA 790 800 810 DGLFARLMGD EVEPRREFIQ DNALSVANLD I

References

[1]

"Genome diversity and DNA divergence of Rhizobium etli." Gonzalez V., Acosta J.L., Santamaria R.I., Bustos P., Hernandez-Gonzalez I.L., Fernandez J.L., Diaz R., Flores M., Mora J., Palacios R., Davila G. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001074 Genomic DNA. Translation: ACE89016.1.
RefSeq	YP_001976194.1. NC_010994.1.
3D structure databases
ProteinModelPortal	B3PVU3.
ModBase	Search...
Protein-protein interaction databases
STRING	B3PVU3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6398312.
GenomeReviews	Gene locus RHECIAT_CH0000013 in contig CP001074_GR.
KEGG	rec:RHECIAT_CH0000013.
PATRIC	23093474. VBIRhiEtl120572_0013.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG304889.
OMA	DCSSRDP.
ProtClustDB	PRK14939.
Family and domain databases
HAMAP	MF_01898. GyrB. [Tree]
InterPro	IPR003594. ATPase-like_ATP-bd. IPR011557. GyrB. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA. IPR013759. Topo_IIA_B/N_ab. IPR002288. Topo_IIA_B_C. IPR013506. Topo_IIA_bsu_dom2. IPR013760. Topo_IIA_cen. IPR018522. TopoIIA_CS. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 2 hits.
KO	K02470.
Pfam	PF00204. DNA_gyraseB. 1 hit. PF00986. DNA_gyraseB_C. 1 hit. PF02518. HATPase_c. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
SMART	SM00387. HATPase_c. 1 hit. SM00433. TOP2c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01059. GyrB. 1 hit.
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3PVU3_RHIE6

Accession

Primary (citable) accession number: B3PVU3

Entry history

Integrated into UniProtKB/TrEMBL:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)