Skip Header

Contribute Send feedback
Read comments (?) or add your own

B3PUJ1 (B3PUJ1_RHIE6) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Carbamoyl-phosphate synthase small chain HAMAP-Rule MF_01209
EC=6.3.5.5 HAMAP-Rule MF_01209
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain HAMAP-Rule MF_01209
Gene names
Name:carA HAMAP-Rule MF_01209 EMBL ACE92072.1
Ordered Locus Names:RHECIAT_CH0003124 EMBL ACE92072.1
OrganismRhizobium etli (strain CIAT 652) [Complete proteome] [HAMAP] EMBL ACE92072.1
Taxonomic identifier491916 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium ›

Protein attributes

Sequence length401 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP-Rule MF_01209

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP-Rule MF_01209

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP-Rule MF_01209 SAAS SAAS006274

Subunit structure

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity. HAMAP-Rule MF_01209

Sequence similarities

Belongs to the CarA family. HAMAP-Rule MF_01209

Contains 1 glutamine amidotransferase type-1 domain. HAMAP-Rule MF_01209

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain207 – 395189Glutamine amidotransferase type-1 By similarity HAMAP-Rule MF_01209
Region1 – 203203CPSase By similarity HAMAP-Rule MF_01209

Sites

Active site2841Nucleophile By similarity HAMAP-Rule MF_01209
Active site3681 By similarity HAMAP-Rule MF_01209
Active site3701 By similarity HAMAP-Rule MF_01209

Sequences

Sequence LengthMass (Da)Tools
B3PUJ1 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: 1BA1D43077DFC716

FASTA40143,085
        10         20         30         40         50         60 
MTATAPWTIE KPTALLVLAD GTVIEGKGIG ATGKIQAEVV FNTALTGYEE IMTDPSYLGQ 

        70         80         90        100        110        120 
IVTFTFPHIG NIGTNDEDIE DLTPAARHGA VGVIFKADIT EPSNYRAAKH LDQWLKARGI 

       130        140        150        160        170        180 
IGLCGIDTRA LTAWIRENGA PNAVIAHDPN GVFDIETLKA EAKAWSGLEG LDLAKIATSG 

       190        200        210        220        230        240 
QSSQWTQTPW IWNEGYGELG AADAKYHVVC LDYGVKRNIL RLFAGLDCKV TVVPATTSAE 

       250        260        270        280        290        300 
DVLAMQPDGI FLSNGPGDPA ATGEYAVPVI KTLVKTDIPV FGICLGHQML GLALGAKTEK 

       310        320        330        340        350        360 
MHQGHHGANH PVKDHTTGKV EIVSMNHGFA VDSKSLPDGV EETHISLFDG TNCGLRVLGK 

       370        380        390        400 
QVFSVQHHPE ASPGPQDSHY LFRRFINMVR EKKGEPALAE R

« Hide

References

[1]"Genome diversity and DNA divergence of Rhizobium etli."
Gonzalez V., Acosta J.L., Santamaria R.I., Bustos P., Hernandez-Gonzalez I.L., Fernandez J.L., Diaz R., Flores M., Mora J., Palacios R., Davila G.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CIAT 652 EMBL ACE92072.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001074 Genomic DNA. Translation: ACE92072.1.
RefSeqYP_001979250.1. NC_010994.1.

3D structure databases

ProteinModelPortalB3PUJ1.
ModBaseSearch...

Protein-protein interaction databases

STRING491916.RHECIAT_CH0003124.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACE92072; ACE92072; RHECIAT_CH0003124.
GeneID6401211.
KEGGrec:RHECIAT_CH0003124.
PATRIC23099738. VBIRhiEtl120572_3100.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0505.
HOGENOMHOG000038087.
KOK01956.
OMARDSHYLF.
ProtClustDBPRK12564.

Enzyme and pathway databases

BioCycRETL491916:GH4T-3123-MONOMER.
UniPathwayUPA00068; UER00171.
UPA00070; UER00115.

Family and domain databases

Gene3D3.50.30.20. 1 hit.
HAMAPMF_01209. CPSase_S_chain.
InterProIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR017926. GATASE.
[Graphical view]
PfamPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMSSF52021. CP_synthsmall. 1 hit.
TIGRFAMsTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB3PUJ1_RHIE6
AccessionPrimary (citable) accession number: B3PUJ1
Entry history
Integrated into UniProtKB/TrEMBL: September 2, 2008
Last sequence update: September 2, 2008
Last modified: May 29, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info