Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Carbamoyl-phosphate synthase small chain

Gene

carA

Organism
Rhizobium etli (strain CIAT 652)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC), Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei284NucleophileUniRule annotation1
Active sitei368UniRule annotation1
Active sitei370UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationImported
Biological processAmino-acid biosynthesis, Arginine biosynthesisUniRule annotation, Pyrimidine biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase small chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chainUniRule annotation
Gene namesi
Name:carAUniRule annotationImported
Ordered Locus Names:RHECIAT_CH0003124Imported
OrganismiRhizobium etli (strain CIAT 652)Imported
Taxonomic identifieri491916 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
Proteomesi
  • UP000008817 Componenti: Chromosome

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotation

Protein-protein interaction databases

STRINGi491916.RHECIAT_CH0003124.

Structurei

3D structure databases

ProteinModelPortaliB3PUJ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini207 – 395Glutamine amidotransferase type-1InterPro annotationAdd BLAST189

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 206CPSaseUniRule annotationAdd BLAST206

Sequence similaritiesi

Belongs to the CarA family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferaseUniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1M. Bacteria.
COG0505. LUCA.
HOGENOMiHOG000038087.
KOiK01956.
OMAiCFNTGMT.
OrthoDBiPOG091H01NP.

Family and domain databases

CDDicd01744. GATase1_CPSase. 1 hit.
Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain. 1 hit.
InterProiView protein in InterPro
IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR035686. CPSase_GATase1.
IPR017926. GATASE.
PfamiView protein in Pfam
PF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
SMARTiView protein in SMART
SM01097. CPSase_sm_chain. 1 hit.
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiView protein in PROSITE
PS51273. GATASE_TYPE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

B3PUJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTATAPWTIE KPTALLVLAD GTVIEGKGIG ATGKIQAEVV FNTALTGYEE
60 70 80 90 100
IMTDPSYLGQ IVTFTFPHIG NIGTNDEDIE DLTPAARHGA VGVIFKADIT
110 120 130 140 150
EPSNYRAAKH LDQWLKARGI IGLCGIDTRA LTAWIRENGA PNAVIAHDPN
160 170 180 190 200
GVFDIETLKA EAKAWSGLEG LDLAKIATSG QSSQWTQTPW IWNEGYGELG
210 220 230 240 250
AADAKYHVVC LDYGVKRNIL RLFAGLDCKV TVVPATTSAE DVLAMQPDGI
260 270 280 290 300
FLSNGPGDPA ATGEYAVPVI KTLVKTDIPV FGICLGHQML GLALGAKTEK
310 320 330 340 350
MHQGHHGANH PVKDHTTGKV EIVSMNHGFA VDSKSLPDGV EETHISLFDG
360 370 380 390 400
TNCGLRVLGK QVFSVQHHPE ASPGPQDSHY LFRRFINMVR EKKGEPALAE

R
Length:401
Mass (Da):43,085
Last modified:September 2, 2008 - v1
Checksum:i1BA1D43077DFC716
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001074 Genomic DNA. Translation: ACE92072.1.
RefSeqiWP_012484607.1. NC_010994.1.

Genome annotation databases

EnsemblBacteriaiACE92072; ACE92072; RHECIAT_CH0003124.
KEGGirec:RHECIAT_CH0003124.

Similar proteinsi

Entry informationi

Entry nameiB3PUJ1_RHIE6
AccessioniPrimary (citable) accession number: B3PUJ1
Entry historyiIntegrated into UniProtKB/TrEMBL: September 2, 2008
Last sequence update: September 2, 2008
Last modified: September 27, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported