B3PUI4 (TDH_RHIE6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-threonine 3-dehydrogenase
EC=1.1.1.103

Gene names

Name:	tdh
Ordered Locus Names:	RHECIAT_CH0003117

Organism

Rhizobium etli (strain CIAT 652) [Complete proteome] [HAMAP]

Taxonomic identifier

491916 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH. HAMAP MF_00627
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00627
Pathway	Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2. HAMAP MF_00627
Subunit structure	Homotetramer By similarity. HAMAP MF_00627
Subcellular location	Cytoplasm By similarity HAMAP MF_00627.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-threonine 3-dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	L-threonine 3-dehydrogenase HAMAP MF_00627		PRO_1000130556
Sites
	Metal binding	42	1	Zinc 1; catalytic By similarity
	Metal binding	67	1	Zinc 1; catalytic By similarity
	Metal binding	97	1	Zinc 2 By similarity
	Metal binding	100	1	Zinc 2 By similarity
	Metal binding	103	1	Zinc 2 By similarity
	Metal binding	111	1	Zinc 2 By similarity
	Metal binding	152	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3PUI4 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: D6AF623474ED712B	FASTA	345	37,504
	10 20 30 40 50 60 MSNMMKALVK SKAEVGLWME DVPVPEVGPN DVLIRVKKSA ICGTDVHIWN WDQWAQKTIP 70 80 90 100 110 120 VPMVVGHEFS GEIAEIGSAV TRYHVGERVS GEGHIVCGKC RNCRAGRGHL CRNTLGVGVN 130 140 150 160 170 180 RPGSFGEFVC IPESNVVPIP DDISDEIAAI FDPFGNAVHT ALSFDLVGED VLVTGAGPIG 190 200 210 220 230 240 IMGALVAKRS GARKVVITDI NPHRLDLARK LGIDHVVDAS KENLADVMKA IGMTEGFDVG 250 260 270 280 290 300 LEMSGAAPAF RDMIDKMNNG GKIAILGIAP AGFEIDWNKV IFKMLNLKGI YGREMFETWY 310 320 330 340 KMIAFVQGGL DLSPIITHRI KIDDFRDGFE AMRSGNSGKV VMDWM

References

[1]

"Genome diversity and DNA divergence of Rhizobium etli." Gonzalez V., Acosta J.L., Santamaria R.I., Bustos P., Hernandez-Gonzalez I.L., Fernandez J.L., Diaz R., Flores M., Mora J., Palacios R., Davila G. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CIAT 652.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001074 Genomic DNA. Translation: ACE92065.1.
RefSeq	YP_001979243.1. NC_010994.1.
3D structure databases
ProteinModelPortal	B3PUI4.
ModBase	Search...
Protein-protein interaction databases
STRING	B3PUI4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6401204.
GenomeReviews	Gene locus tdh in contig CP001074_GR.
KEGG	rec:RHECIAT_CH0003117.
PATRIC	23099724. VBIRhiEtl120572_3093.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG753318.
OMA	RDGFEAM.
ProtClustDB	PRK05396.
Family and domain databases
HAMAP	MF_00627. Thr_dehydrog. [Tree]
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR004627. L-Threonine_3-DHase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00060.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
TIGRFAMs	TIGR00692. Tdh. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TDH_RHIE6

Accession

Primary (citable) accession number: B3PUI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program