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B3PS46 (SYL_RHIE6) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:RHECIAT_CH0004409
OrganismRhizobium etli (strain CIAT 652) [Complete proteome] [HAMAP]
Taxonomic identifier491916 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Protein attributes

Sequence length876 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 876876Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091350

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif632 – 6365"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6351ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B3PS46 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: 486DEF4C54850C8F

FASTA87697,751
        10         20         30         40         50         60 
MATERYNPRD AEPRWQQKWN EDKVFETDNA DPREKYYVLE MFPYPSGRIH MGHVRNYAMG 

        70         80         90        100        110        120 
DVVARYKRAR GYNVLHPMGW DAFGMPAENA AMERGVHPAS WTYQNIASMK AQLKAMGLSL 

       130        140        150        160        170        180 
DWSREFATCD VEYYQHQQHL FLDFLEKGLV YRKQSKVNWD PVDNTVLANE QVIDGRGWRS 

       190        200        210        220        230        240 
GALVEQRELT QWFFKITDFS QDLLDALDTL DQWPEKVRLM QKNWIGRSEG LTVRWEIVPE 

       250        260        270        280        290        300 
TAPAGETEIT VYTTRPDTLF GASFLAIAAD HPLAKDAAAK NVEIEAFCEE CRRAGTSLAA 

       310        320        330        340        350        360 
LETAEKKGLD TGIRVRHPLD PTWELPVYIA NFVLMDYGTG AIFGCPSGDQ RDLDFARKYG 

       370        380        390        400        410        420 
LPVVAVVMPS DGDAASFAVG DTAYDGDGVM INSRFLDGKT TEEAFNIVAD RLSAASLGNT 

       430        440        450        460        470        480 
PVAERKVNFR LRDWGISRQR YWGCPIPVIH CDACGVVPVP KTDLPVKLPE DVTFDQPGNP 

       490        500        510        520        530        540 
LDRHPTWRHV TCPHCGKDAR RETDTMDTFV DSSWYFTRFT APWEAKPTDP EAANRWLPVD 

       550        560        570        580        590        600 
QYIGGIEHAI LHLLYSRFFT RAMRETGHVA ASEPFKGLFT QGMVVHETYS RKAGAGREWV 

       610        620        630        640        650        660 
APADIRIEEI DGKRRALLLA TGEEVAIGSI EKMSKSKKNV VDPDDIIASY GADTARFFVL 

       670        680        690        700        710        720 
SDSPPERDVI WSEAGVEGAH RFTQRLWRLI SEAAGVLSTV AAAPASEGEA LAISQAAHKT 

       730        740        750        760        770        780 
LKAVQNDYDK LWFNKAVARI YELVNALAAP LTKVAAGEGD IAYRAAVRDA AEILIQLVAP 

       790        800        810        820        830        840 
MTPHLAEECW ATLGNTGLLA RTGWPRFVEA LVVENDVVLP VQINGKKRAE LTISRDADQN 

       850        860        870 
AVTDAVLELD AVKNVLNGQA PKKIIVVPQR IVNIVV 

« Hide

References

[1]"Genome diversity and DNA divergence of Rhizobium etli."
Gonzalez V., Acosta J.L., Santamaria R.I., Bustos P., Hernandez-Gonzalez I.L., Fernandez J.L., Diaz R., Flores M., Mora J., Palacios R., Davila G.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CIAT 652.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001074 Genomic DNA. Translation: ACE93336.1.
RefSeqYP_001980514.1. NC_010994.1.

3D structure databases

ProteinModelPortalB3PS46.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING491916.RHECIAT_CH0004409.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACE93336; ACE93336; RHECIAT_CH0004409.
GeneID6402496.
KEGGrec:RHECIAT_CH0004409.
PATRIC23102302. VBIRhiEtl120572_4362.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycRETL1328306-WGS:GSTH-4229-MONOMER.
RETL491916:GH4T-4408-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RHIE6
AccessionPrimary (citable) accession number: B3PS46
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 2, 2008
Last modified: July 9, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries