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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Rhizobium etli (strain CIAT 652)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei635 – 6351ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-4229-MONOMER.
RETL491916:GH4T-4408-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:RHECIAT_CH0004409
OrganismiRhizobium etli (strain CIAT 652)
Taxonomic identifieri491916 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
ProteomesiUP000008817: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 876876Leucine--tRNA ligasePRO_1000091350Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi491916.RHECIAT_CH0004409.

Structurei

3D structure databases

ProteinModelPortaliB3PS46.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi632 – 6365"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3PS46-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATERYNPRD AEPRWQQKWN EDKVFETDNA DPREKYYVLE MFPYPSGRIH
60 70 80 90 100
MGHVRNYAMG DVVARYKRAR GYNVLHPMGW DAFGMPAENA AMERGVHPAS
110 120 130 140 150
WTYQNIASMK AQLKAMGLSL DWSREFATCD VEYYQHQQHL FLDFLEKGLV
160 170 180 190 200
YRKQSKVNWD PVDNTVLANE QVIDGRGWRS GALVEQRELT QWFFKITDFS
210 220 230 240 250
QDLLDALDTL DQWPEKVRLM QKNWIGRSEG LTVRWEIVPE TAPAGETEIT
260 270 280 290 300
VYTTRPDTLF GASFLAIAAD HPLAKDAAAK NVEIEAFCEE CRRAGTSLAA
310 320 330 340 350
LETAEKKGLD TGIRVRHPLD PTWELPVYIA NFVLMDYGTG AIFGCPSGDQ
360 370 380 390 400
RDLDFARKYG LPVVAVVMPS DGDAASFAVG DTAYDGDGVM INSRFLDGKT
410 420 430 440 450
TEEAFNIVAD RLSAASLGNT PVAERKVNFR LRDWGISRQR YWGCPIPVIH
460 470 480 490 500
CDACGVVPVP KTDLPVKLPE DVTFDQPGNP LDRHPTWRHV TCPHCGKDAR
510 520 530 540 550
RETDTMDTFV DSSWYFTRFT APWEAKPTDP EAANRWLPVD QYIGGIEHAI
560 570 580 590 600
LHLLYSRFFT RAMRETGHVA ASEPFKGLFT QGMVVHETYS RKAGAGREWV
610 620 630 640 650
APADIRIEEI DGKRRALLLA TGEEVAIGSI EKMSKSKKNV VDPDDIIASY
660 670 680 690 700
GADTARFFVL SDSPPERDVI WSEAGVEGAH RFTQRLWRLI SEAAGVLSTV
710 720 730 740 750
AAAPASEGEA LAISQAAHKT LKAVQNDYDK LWFNKAVARI YELVNALAAP
760 770 780 790 800
LTKVAAGEGD IAYRAAVRDA AEILIQLVAP MTPHLAEECW ATLGNTGLLA
810 820 830 840 850
RTGWPRFVEA LVVENDVVLP VQINGKKRAE LTISRDADQN AVTDAVLELD
860 870
AVKNVLNGQA PKKIIVVPQR IVNIVV
Length:876
Mass (Da):97,751
Last modified:September 2, 2008 - v1
Checksum:i486DEF4C54850C8F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001074 Genomic DNA. Translation: ACE93336.1.
RefSeqiYP_001980514.1. NC_010994.1.

Genome annotation databases

EnsemblBacteriaiACE93336; ACE93336; RHECIAT_CH0004409.
GeneIDi6402496.
KEGGirec:RHECIAT_CH0004409.
PATRICi23102302. VBIRhiEtl120572_4362.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001074 Genomic DNA. Translation: ACE93336.1.
RefSeqiYP_001980514.1. NC_010994.1.

3D structure databases

ProteinModelPortaliB3PS46.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi491916.RHECIAT_CH0004409.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACE93336; ACE93336; RHECIAT_CH0004409.
GeneIDi6402496.
KEGGirec:RHECIAT_CH0004409.
PATRICi23102302. VBIRhiEtl120572_4362.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-4229-MONOMER.
RETL491916:GH4T-4408-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome diversity and DNA divergence of Rhizobium etli."
    Gonzalez V., Acosta J.L., Santamaria R.I., Bustos P., Hernandez-Gonzalez I.L., Fernandez J.L., Diaz R., Flores M., Mora J., Palacios R., Davila G.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CIAT 652.

Entry informationi

Entry nameiSYL_RHIE6
AccessioniPrimary (citable) accession number: B3PS46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 2, 2008
Last modified: February 4, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.