B3PRI2 (SYGB_RHIE6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase beta subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase beta subunit
Short name=GlyRS

Gene names

Name:	glyS
Ordered Locus Names:	RHECIAT_CH0000980

Organism

Rhizobium etli (strain CIAT 652) [Complete proteome] [HAMAP]

Taxonomic identifier

491916 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium › Rhizobium etli

Protein attributes

Sequence length	704 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP-Rule MF_00255
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: InterPro glycine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 704	704	Glycine--tRNA ligase beta subunit HAMAP-Rule MF_00255		PRO_1000101319

Sequences

	Sequence		Length	Mass (Da)
	B3PRI2 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: AD93C3C49DEF3B0C	FASTA	704	77,176
	10 20 30 40 50 60 MPNLLLELRS EEIPARMQRK AAGDLKKLVT DALVEAGLSY EGAREYWTPR RLALDIHGLT 70 80 90 100 110 120 ARSADVREER KGPRTDANEK AIEGFLRGAG LSSVSEAQVV SDPKKGDFYV AVISKPGRAT 130 140 150 160 170 180 EEIVAEVMPG IIRDFPWPKS MRWGKASSKS GALRWVRPLQ SIVCTFGPEH EETTVIPFEI 190 200 210 220 230 240 DGITASNITY GHRFHAPEAI TVRRFDDYAA SLEKAKVILD AERRKDIILH DARDIAFANG 250 260 270 280 290 300 LELVEDEGLL EEVSGLVEWP QVLMGSFEED YLSIPSEIIR LTIKTNQKCF VTRKQGEDTL 310 320 330 340 350 360 SNRFILVSNI QAHDGGKEIV HGNGKVVRAR LSDALHFWKR DQGNLPDLET LAASAAKFGL 370 380 390 400 410 420 DLQKPLDQRM AKLDALDVTF HAKLGTQGAR VARIRALAKE LAAITGADPA LTDRAAVLAK 430 440 450 460 470 480 ADLRTEAVGE FPELQGLMGR KYAVLQGENA SVAAAVEDHY KPQGPSDRVP EDKVAITLAL 490 500 510 520 530 540 ADKLDTLTGF WAIDEKPTGS KDPFALRRAA LGVVRILLER RVRLPLLATT RDGDLLSFFH 550 560 570 580 590 600 DRLKVYLRDQ GARYDLIDAV LTPDADDLLM VARRVEALTA FITSEDGKNL LAGTKRATQL 610 620 630 640 650 660 LAAEEKKGTV IADGVSPALL KLDAEKELFA AISSASKDAA DAVAGEDFRS AMEALSKLRG 670 680 690 700 PVDRFFEEVL VNDEDAAIRA NRLALLRLIR EATGTVADFS KISG

References

[1]

"Genome diversity and DNA divergence of Rhizobium etli." Gonzalez V., Acosta J.L., Santamaria R.I., Bustos P., Hernandez-Gonzalez I.L., Fernandez J.L., Diaz R., Flores M., Mora J., Palacios R., Davila G. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CIAT 652.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001074 Genomic DNA. Translation: ACE89965.1.
RefSeq	YP_001977143.1. NC_010994.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	491916.RHECIAT_CH0000980.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACE89965; ACE89965; RHECIAT_CH0000980.
GeneID	6399067.
KEGG	rec:RHECIAT_CH0000980.
PATRIC	23095403. VBIRhiEtl120572_0961.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0751.
HOGENOM	HOG000264304.
KO	K01879.
OMA	QMNAVAD.
ProtClustDB	PRK01233.
Enzyme and pathway databases
BioCyc	RETL491916:GH4T-2732-MONOMER.
Family and domain databases
HAMAP	MF_00255. Gly_tRNA_synth_beta.
InterPro	IPR008909. DALR_anticod-bd. IPR015944. Gly-tRNA-synth_bsu. IPR006194. Gly-tRNA-synth_heterodimer. [Graphical view]
Pfam	PF05746. DALR_1. 1 hit. PF02092. tRNA_synt_2f. 1 hit. [Graphical view]
PRINTS	PR01045. TRNASYNTHGB.
TIGRFAMs	TIGR00211. glyS. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGB_RHIE6

Accession

Primary (citable) accession number: B3PRI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 2, 2008
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program